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Quality control method for 10 index components of traditional Chinese medicine conquering prescription for treating lung cancer

A quality control method, the technology of gram square, applied in the field of traditional Chinese medicine, can solve the problems of lack of standard products, no quality control methods of multi-index components, etc., and achieve the effect of high detection sensitivity, simple method, accurate and reliable data

Inactive Publication Date: 2015-03-25
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology

In the actual process of multi-index component determination of traditional Chinese medicine preparations, the insurmountable bottleneck is the lack of some standard products, which directly limits the idea of ​​using conventional external standard method or internal standard method to achieve multi-index quality control.
The material basis of the anti-lung cancer effect of Ke formula has been systematically studied, and the active ingredients of flavonoids have been clarified [Dong Jing, Yin Lian, Zhang Xu, et al. Zhou Shi Ke Jinyan formula anti-lung cancer A9 cell effective part I component analysis research, China Zhong Journal of Medical Information, 2009, 16(11): 40-42; Sun Yi, Zhao Qin, Yin Lian, et al. Research on Anti-proliferation of A549 Cells Extracted from Zhoushi Kejinyan Formula, Journal of Nanjing University of Traditional Chinese Medicine, 200925(2 ): 107-109.], but the preparation and related medicinal materials have no quality control methods for multi-index components

Method used

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  • Quality control method for 10 index components of traditional Chinese medicine conquering prescription for treating lung cancer
  • Quality control method for 10 index components of traditional Chinese medicine conquering prescription for treating lung cancer
  • Quality control method for 10 index components of traditional Chinese medicine conquering prescription for treating lung cancer

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Effect test

Embodiment 1

[0073] Embodiment 1: The method for quality control of 10 index components of traditional Chinese medicine Kefang medicinal materials used for the treatment of lung cancer, the steps are as follows:

[0074] (1) preparation of gram side medicinal material test sample solution:

[0075] Precisely weigh 5 parts of Cat's Claw, 5 parts of Golden Buckwheat, 5 parts of Houttuynia cordata, 5 parts of Agrimony, 5 parts of Winter Melon Seeds, 10 parts of Shanzi Mushroom, 10 parts of Sumac, 10 parts of Safflower, 10 parts of Pinellia 20g, 5 parts of Dannanxing, 5 parts of Radix Glycyrrhizae, 5 parts of Polygonatum Polygonatum, 5 parts of Ophiopogon japonicus, 20g in total, crushed, added 100ml of 50% ethanol, heated and refluxed for 0.5h, filtered, extracted 3 times, and the filtrate was concentrated to 40ml under reduced pressure. Take 5ml and spin dry, dissolve with 70% methanol and dilute to 25ml, pass through a 0.45μm filter membrane, and use it as the test solution of the gram squa...

Embodiment 2

[0089] Embodiment 2: The method for quality control of 10 index components of traditional Chinese medicine Kefang medicinal materials used for the treatment of lung cancer, the steps are as follows:

[0090] (1) preparation of gram side medicinal material test sample solution:

[0091] Precisely weigh 15 parts of Cat's Claw, 15 parts of Golden Buckwheat, 15 parts of Houttuynia cordata, 11 parts of Agrimony, 15 parts of Winter Melon Seeds, 20 parts of Shanzi Mushroom, 20 parts of Sumac, 20 parts of Safflower, 20 parts of Pinellia 15 parts of Dannanxing, 15 parts of Radix Ginseng, 15 parts of Polygonatum Polygonatum, 15 parts of Ophiopogon japonicus, 40g in total, crushed, added 400ml of 80% ethanol, heated and refluxed for 1.5h, filtered, extracted 3 times, and the filtrate was concentrated to 80ml under reduced pressure. Take 10ml and spin dry, dissolve with 70% methanol and dilute to 25mL, pass through a 0.45μm filter membrane, and use it as the test solution of Kefang medici...

Embodiment 3

[0105] Embodiment 3: The quality control method of 10 index components of traditional Chinese medicine Kefang medicinal materials used for the treatment of lung cancer, the steps are as follows:

[0106] (1) preparation of gram side medicinal material test sample solution:

[0107] 10 parts of Cat's Claw, 8 parts of Golden Buckwheat, 9 parts of Houttuynia cordata, 15 parts of Agrimony, 12 parts of Winter Melon Seeds, 13 parts of Shanzi Mushroom, 16 parts of Sappan, 13 parts of Safflower, 15 parts of Pinellia, gallbladder 9 parts of Southern Star, 8 parts of Adenophora, 7 parts of Polygonatum Polygonatum, and 9 parts of Ophiopogon japonicus, 30g in total, were crushed, added 300ml of 75% ethanol, heated and refluxed for 1.0h, filtered, extracted 3 times, the filtrate was concentrated under reduced pressure to 60ml, and 8ml was spun Dry it, dissolve it with 70% methanol and dilute it to 25ml, pass through a 0.45μm filter membrane, and use it as the test solution of the gram squa...

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Abstract

The invention discloses a quality control method for 10 index components of a traditional Chinese medicine conquering prescription for treating lung cancer. The method comprises the following steps: (1) extracting a test solution for preparing medicinal materials of the conquering prescription or preparation; (2) detecting hybrid reference substances catechinic acid, hyperin, rutin, isoquercitrin, galuteolin, quercitrin, apigenin-7-O-glucoside and quercetin chromatogram by adopting a liquid chromatography, and respectively calculating relative correction factors fkm of other components relatively to the quercetin by employing the quercetin as an internal standard compound; and (3) detecting the chromatogram of the test solution of the conquering prescription by the liquid chromatography, calculating the content according to the regression equation by employing the quercetin as an internal standard, and calculating the contents of other 9 components according to the peak area and the relative correction factors. Compared with the internal standard method, the method is accurate and reliable in data, high in detection sensitivity, and simple and convenient in method, and has relatively good adaptability on different model numbers of high performance liquid chromatographs and chromatographic columns; the problem that the medicinal material of the conquering prescription and the preparation quality of the medicinal material cannot be objectively and reasonably controlled due to shortage of reference substances can be solved; and the quality control method has important significance on control of the medicinal materials of the conquering prescription and the preparation quality and guarantee of the curative effect.

Description

1. Technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for quality control of 10 index components of a traditional Chinese medicinal prescription for treating lung cancer. 2. Background technology [0002] Lung cancer is a malignant tumor that seriously threatens human life and health. In recent years, the incidence rate has shown an obvious upward trend. More than 1 million people die of lung cancer worldwide every year. Traditional Chinese medicine plays an important role in inhibiting or killing tumor cells and postoperative conditioning, reducing adverse reactions of radiotherapy and chemotherapy, improving patients' symptoms and signs, and improving quality of life. Ke Jinyan Fang (Ke Fang) is an anti-lung cancer clinical experience prescription. Composition of Ginseng, Polygonatum Polygonatum, and Ophiopogon japonicus [Yin Lian, Zhang Xu, Ye Lihong. A traditional Chinese medicine extract and it...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 尹莲张旭吴芳
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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