DNA Barcoding-based identification kit for pelteobagrus fulvidraco, ictalurus nebulosus, weever and biological products of pelteobagrus fulvidraco, ictalurus nebulosus and weever

Abstract

The invention discloses a DNA Barcoding-based identification method for pelteobagrus fulvidraco, ictalurus nebulosus, weever and biological products of yellow-head catfish meat, ictalurus nebulosus and weever. The three fishes and uncooked meat products of the three fishes are identified according to lengths of edited base sequences, the method is established based on DNA Barcoding and is not limited by morphology of identification materials, and accurate conclusion can be made even only individual tissue fragments are available, so that the DNA Barcoding-based identification method can be widely applied to accurate identification on fishes and uncooked meat products of fishes.

Description

technical field [0001] The invention discloses a method for identifying fish, in particular to a method for identifying yellow catfish, cloud catfish and perch based on DNA Barcoding. Background technique [0002] As an indicator organism of the aquatic environment, fish can not only be used as an indicator of water purification, but also as an indicator of alien species invasion. At the same time, fish diversity indicators can also reflect their habitat status. In recent years, with the rapid development of my country's economy, ecological problems have become increasingly prominent, which provides convenient conditions for the invasion of alien species and brings hidden worries to the development and protection of local fish. How to quickly and effectively identify fish has become an urgent problem to be solved. However, because the traditional morphological taxonomy is not paid enough attention at present, the professionals of morphological taxonomy are gradually decreas...

AI Technical Summary

Problems solved by technology

However, because the traditional morphological taxonomy is not paid enough attention at present, the professionals of morphological taxonomy are gradually decreasing, which affects the work of fish resource protection and habitat research.

[0003] CN200510022323.1, a rapid identification method of fish molecular biology developed by Sichuan University in recent years, combines the conservation of nuclear DNA sequences encoding fish RNA and the interspecies differences of transcriptional spacers with the traditional Electrophoresis technology to identify fish, this method is time-consuming and costly, more importantly, this method cannot be used to screen and identify specific fish