Six common fish identification kits based on DNA Barcoding

Abstract

The invention discloses an identification method of six common fishes (mandarin fish, large yellow croaker, grass carp, silver carp, crucian and carp). The six common fishes and raw fish products thereof are identified by comparing the lengths of base sequences compiled. The method is established based on DNA Barcoding and is not limited by shape of the identified material. Even if the individual is a tissue fragment, an accurate result can be obtained. Therefore, the kit can be widely applied to accurately identifying fishes and raw fish products thereof.

Description

technical field [0001] The invention discloses a method for identifying fish, in particular to an identification method for six common fish species (mandarin fish, large yellow croaker, grass carp, silver carp, crucian carp and carp). Background technique [0002] As an indicator organism of the aquatic environment, fish can not only be used as an indicator of water purification, but also as an indicator of alien species invasion. At the same time, fish diversity indicators can also reflect their habitat status. In recent years, with the rapid development of my country's economy, ecological problems have become increasingly prominent, which provides convenient conditions for the invasion of alien species and brings hidden worries to the development and protection of local fish. How to quickly and effectively identify fish has become an urgent problem to be solved. However, because the traditional morphological taxonomy is not paid enough attention at present, the profession...

AI Technical Summary

Problems solved by technology

However, because the traditional morphological taxonomy is not paid enough attention at present, the professionals of morphological taxonomy are gradually decreasing, which affects the work of fish resource protection and habitat research.

[0003] CN200510022323.1, a rapid identification method of fish molecular biology developed by Sichuan University in recent years, combines the conservation of nuclear DNA sequences encoding fish RNA and the interspecies differences of transcriptional spacers with the traditional Electrophoresis technology to identify fish, this method is time-consuming and costly, more importantly, this method cannot be used to screen and identify specific fish

Images