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Prunus apetala chloroplast genome and application thereof

A chloroplast genome, T-shaped technology, applied in the field of genes

Active Publication Date: 2019-09-27
ZHEJIANG FORESTRY ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the chloroplast genome of T. japonica is still blank

Method used

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  • Prunus apetala chloroplast genome and application thereof
  • Prunus apetala chloroplast genome and application thereof
  • Prunus apetala chloroplast genome and application thereof

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Embodiment Construction

[0033] The present invention will be described in detail through specific examples below. These embodiments are provided for a more thorough understanding of the present invention and to fully convey the scope of the present invention to those skilled in the art.

[0034] "Includes" or "comprises" mentioned throughout the specification and claims is an open term, so it should be interpreted as "including but not limited to". The subsequent description in the specification is a preferred implementation mode for implementing the present invention, but the description is for the purpose of the general principles of the specification, and is not intended to limit the scope of the present invention. The scope of protection of the present invention should be defined by the appended claims.

[0035] Unless otherwise specified, various reagents and materials used in the present invention can be purchased from the market.

[0036] 1. Extract the total DNA of the sample

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Abstract

The invention discloses a prunus apetala chloroplast genome for the first time. The prunus apetala chloroplast genome is applied to a phylogenetic study of prunus apetala, and a phylogenetic status of the prunus apetala in prunus is analyzed. A phylogenetic relationship of a prunus subgenus species is established through the prunus apetala chloroplast genome by methods of molecular systematics and phylogenetic genomics; on the basis, the whole chloroplast genome is used as a super DNA (deoxyribonucleic acid) bar code of a prunus subgenus for identifying an oriental cherry variety; a technical support is provided for protection of an oriental cherry germplasm resource and species identification; and the chloroplast genome has an important significance for evaluation, protection and utilization of seed breeding and genetic diversity of a typical prunus subgenus plant.

Description

technical field [0001] The invention belongs to the field of gene technology, and in particular relates to the chloroplast genome of T. japonica and its application. Background technique [0002] With the development of society, human beings have increasingly urgent needs for the exploration of biodiversity, the rational use of biological resources and the rapid identification of species, and DNA barcoding technology has emerged as the times require. Originally, a DNA barcode referred to a standard DNA sequence used for species identification. The father of DNA barcoding, Hebert et al., successfully identified 200 species of Lepidoptera insects using a fragment of the mitochondrial cytochrome oxidase subunit 1 (cytochromeoxidase I, COI) gene of about 680 bp, and proposed DNA fragments for the first time as distinguishing species. Similar to the concept of DNA barcodes that distinguish goods by barcodes. Each species has a DNA sequence that distinguishes it from other speci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/12C12Q1/6895C12Q1/6869
CPCC12Q1/6869C12Q1/6895C12Q2535/122C12Q2537/165
Inventor 沈鑫蒋冬月柳新红
Owner ZHEJIANG FORESTRY ACAD
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