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Extraction method and application of component group of traditional Chinese medicine extract for treating hemorrhagic stroke

A technology of hemorrhagic stroke and extraction method, which is applied in the field of traditional Chinese medicine and can solve the problems such as insufficient curative effect

Inactive Publication Date: 2015-03-25
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current Western medicine treatment methods for cerebral hemorrhage fall into two categories: surgical treatment and comprehensive medical treatment, which have certain risks and the curative effect is not significant enough.

Method used

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  • Extraction method and application of component group of traditional Chinese medicine extract for treating hemorrhagic stroke
  • Extraction method and application of component group of traditional Chinese medicine extract for treating hemorrhagic stroke
  • Extraction method and application of component group of traditional Chinese medicine extract for treating hemorrhagic stroke

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1: The extraction method of the Chinese medicine extract component group for the treatment of hemorrhagic stroke, the extraction steps are as follows:

[0041] (1) Raw materials: 1 part of rhubarb, 1 part of paeonol, 1 part of red peony root, 1 part of raw land, 1 part of notoginseng;

[0042] (2) Pulverize the above-mentioned crude drug to 20 mesh, add 5 times the amount of water to extract twice, combine the filtrate, concentrate to 0.5g crude drug / ml, extract 3 times with 1 times the amount of dichloromethane, discard the dichloromethane layer, water The layer is then extracted 3 times with 1 times the amount of n-butanol, and the combined n-butanol extract is recovered and dried as a Chinese medicine extract component group;

[0043] (3) Analysis of the components of Chinese medicine extracts: HPLC-MS was used to analyze the chemical components of Chinese medicine extracts, mainly consisting of paeoniflorin, tannins, paeonol glycosides, anthraquinones, stilb...

Embodiment 2

[0044] Embodiment 2: The extraction method of the Chinese medicine extract component group for the treatment of hemorrhagic stroke, the extraction steps are as follows:

[0045] (1) Raw materials: 2 parts of rhubarb, 3 parts of paeonol, 3 parts of red peony, 5 parts of raw land, 3 parts of Panax notoginseng;

[0046] (2) Pulverize the above crude drug to 30 mesh, add 10 times the amount of water to extract twice, combine the filtrate, concentrate to 1.0g crude drug / ml, extract 4 times with 2 times the amount of dichloromethane, discard the dichloromethane layer, water The layer is extracted 4 times with 2 times the amount of n-butanol, and the combined n-butanol extract is recovered and dried as the Chinese medicine extract component group;

[0047] (3) Analysis of the components of Chinese medicine extracts: HPLC-MS was used to analyze the chemical components of Chinese medicine extracts, mainly consisting of paeoniflorin, tannins, paeonol glycosides, anthraquinones, stilbene...

Embodiment 3

[0048] Example 3: The extraction method of the Chinese medicine extract component group for the treatment of hemorrhagic stroke, the extraction steps are as follows:

[0049] (1) Raw materials: 5 parts of rhubarb, 5 parts of paeonol, 5 parts of red peony, 8 parts of raw land, 6 parts of Sanqi;

[0050](2) Pulverize the above crude drug to 40 mesh, add 15 times the amount of water to extract twice, combine the filtrate, concentrate to 1.5g crude drug / ml, extract 6 times with 3 times the amount of dichloromethane, discard the dichloromethane layer, water The layer was extracted 6 times with 3 times the amount of n-butanol, and the combined n-butanol extract was recovered and dried as the Chinese medicine extract component group;

[0051] (3) Analysis of the components of Chinese medicine extracts: HPLC-MS was used to analyze the chemical components of Chinese medicine extracts, mainly consisting of paeoniflorin, tannins, paeonol glycosides, anthraquinones, stilbenes, saponins an...

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Abstract

The invention discloses an extraction method and an application of a component group of a traditional Chinese medicine extract for treating hemorrhagic stroke. The extraction method comprises the following steps: after crushing rheum officinale, cortex moutan, red peony roots, dried radix rehmanniae and pseudo-ginseng in a certain weight ratio, adding water to decoct and extract; after extracting an extraction liquid by using dichloromethane, extracting a water layer by using n-butyl alcohol; and recovering and drying the n-butyl alcohol extraction liquid to obtain the component group of the traditional Chinese medicine extract. Analyzed by HPLC-MS, the traditional Chinese medicine extract is prepared from 33 components: paeoniflorins, tannins, paeonosides, anthraquinones, stilbenes, saponins and iridoid glycosides and can be used for remarkably reducing levels of neuronspecific enolase of essential hypertension male rats, S-100beta proteins, IL-1, TNF-alpha and plasma fibrinogen and can be used for preparing the medicine for treating hemorrhagic stroke.

Description

1. Technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for extracting component groups of Chinese medicine extracts for treating hemorrhagic stroke and its application. 2. Background technology [0002] Hemorrhagic stroke (cerebral hemorrhage) is a common clinical disease at home and abroad. The disease has sudden onset, rapid progress, and high fatality rate. The prognosis of patients directly affects the quality of life after the disease. Neuron-specific enolase (SNE) and S-100β protein are important indicators for judging the severity of brain damage. Among them, SNE is a marker enzyme of neurons and neuroendocrine cells. When ischemia and hypoxia occur in brain tissue, Elevated levels of SNE in cerebrospinal fluid. S-100β protein is a brain-specific protein, and the higher the level of S-100β protein in serum, the more severe the brain damage. The current Western medicine treatment methods for c...

Claims

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Application Information

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IPC IPC(8): A61K36/804A61P9/10A61P7/04
CPCA61K36/258A61K36/708A61K36/71A61K36/804A61K2236/15A61K2236/331A61K2236/39A61K2236/55A61K2300/00
Inventor 尹莲李国春黄西吴勉华程海波
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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