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Preparation method of growth-promoting synbiotic bacterium liquid and application

A synbiotic and growth-promoting technology, which is applied to the preparation of growth-promoting synbiotics, the preparation of Lactobacillus casei-inulin-astragalus polysaccharide synbiotic bacteria liquid, and the field of microecological regulators, which can solve many problems, the preparation of Lactobacillus casei There are fewer problems such as method research to achieve the effect of inhibiting survival, strong inhibition, and high immune activity

Active Publication Date: 2015-03-25
沈阳博睿农牧科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are not many studies on Lactobacillus casei in my country at present, and there are fewer studies on the preparation and application of Lactobacillus casei as a probiotic and prebiotic to form a synbiotic

Method used

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  • Preparation method of growth-promoting synbiotic bacterium liquid and application
  • Preparation method of growth-promoting synbiotic bacterium liquid and application
  • Preparation method of growth-promoting synbiotic bacterium liquid and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The pH value of the MRS medium was adjusted with 1 mol / L NaOH to make it 6.6. Autoclave for 20 minutes. Put 100mL of MRS liquid medium into a 150mL Erlenmeyer flask, add 1g of inulin and astragalus polysaccharide complex, including 0.3g of inulin and 0.7g of astragalus polysaccharide, and then filter with a 0.22μm microporous membrane to separate the filtrate. Under the aseptic operating table, inoculate the activated Lactobacillus casei into the filtrate of the above-mentioned MRS medium containing the inulin-astragalus polysaccharide complex at an inoculum size of 2% by volume, divide and seal. The above-mentioned filtrate containing Lactobacillus casei was placed statically in an anaerobic incubator at 37°C for 25h. After breeding, the yellow bacterial liquid is the Lactobacillus casei-inulin-astragalus polysaccharide synbiotic bacterial liquid.

[0020] figure 1 Show the growth curve of Lactobacillus casei under static and shaker culture state; figure 2 The gro...

Embodiment 2

[0026] The pH value of the MRS medium was adjusted with 1 mol / L NaOH to make the pH value 6.5. Autoclave for 20 minutes. Put 100mL of MRS liquid medium into a 150mL Erlenmeyer flask, add 1g of inulin and astragalus polysaccharide complex, including 0.2g of inulin and 0.8g of astragalus polysaccharide, and then filter with a 0.22μm microporous membrane to separate the filtrate. Under the aseptic operating table, inoculate the activated Lactobacillus casei into the filtrate of the above-mentioned MRS medium containing the inulin-astragalus polysaccharide complex at an inoculum size of 2% by volume, divide and seal. The above-mentioned filtrate containing Lactobacillus casei was placed statically in an anaerobic incubator at 37°C for 24 hours. After breeding, the yellow bacterial liquid is the Lactobacillus casei-inulin-astragalus polysaccharide synbiotic bacterial liquid.

[0027] The number of viable bacteria in the Lactobacillus casei-inulin-Astragalus polysaccharide synbiot...

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Abstract

The invention relates to a preparation method of growth-promoting synbiotic bacterium liquid and application. The preparation method comprises the steps of adding complex of synanthrin and astragalus polysaccharide into an MRS (Methicillin Resistant Staphylococcus) culture medium at a proper ratio, filtering and separating by adopting a microporous filtering membrane and collecting a filtrate; inoculating activated lactobacillus casei on the filtrate of the culture medium containing the complex of the synanthrin and the astragalus polysaccharide, and placing in an anaerobic incubator with temperature of 37 DEG C for standing culture, thus obtaining the synbiotic bacterium liquid containing the synanthrin, the astragalus polysaccharide and the activated lactobacillus casei; the synbiotic is added into complete feed and the effect of feeding and mixing simultaneously can be achieved; the synanthrin and the astragalus polysaccharide can provide nutrition for the lactobacillus casei in intestinal tracts and generate the acid environment to enhance multiplication of beneficial bacteria, and by complementary relation of the synanthrin, the astragalus polysaccharide and the lactobacillus casei, the inhibiting action on pathogenic microorganisms is strong; defense mechanisms of organisms can be resisted, the intestinal tracts of the organisms can be adjusted to maintain the healthy state; the immunocompetence is high, so that the quantity of pathogenic bacteria is reduced and the existence of pathogenic harmful bacteria and exogenous germs can be inhibited.

Description

technical field [0001] The invention relates to an animal additive, in particular to a preparation method and application of a growth-promoting synbiotic, belonging to a microecological regulator. Specifically, it relates to the preparation method and application of Lactobacillus casei-inulin-astragalus polysaccharide synbiotic bacterial liquid. Background technique [0002] When animals suffer from various external or internal factors such as disease and weaning, the immune function is often reduced and the structure of intestinal microbial flora changes (the number of beneficial bacteria will decrease and the number of harmful bacteria will increase), resulting in low resistance , diarrhea, and eventually lead to decreased production performance. The use of antibiotics and chemical additives did have an obvious growth-promoting effect for a period of time, but problems such as drug residues and drug resistance followed. Nowadays, food safety is more and more valued by co...

Claims

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Application Information

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IPC IPC(8): A23K1/16A23K1/00
Inventor 陈静刘显军李建涛杨璐鲁茗源
Owner 沈阳博睿农牧科技有限公司
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