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Bacillus tequilensis and its use in prevention and control of fusarium graminearum

A technology of Bacillus tequila and Fusarium can be applied in application, microorganism-based methods, bacteria and other directions, which can solve the problems of increasing control difficulty and lack of ideal high-resistant wheat varieties, and achieve the effect of good application prospects.

Active Publication Date: 2015-03-11
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] For many years, the control of wheat head blight has mainly relied on chemical pesticides, but with the environmental problems caused by chemical pesticides, coupled with years of drug use has led to the emergence of pathogenic bacteria resistant Fusarium graminearum strains (Changjun Chen et al., 2007), make it more difficult to prevent
Although a lot of energy has been invested in related research at home and abroad on the quality of scab resistance, there is still no ideal high-resistant wheat variety (Li Zhenghui et al., 2007).

Method used

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  • Bacillus tequilensis and its use in prevention and control of fusarium graminearum
  • Bacillus tequilensis and its use in prevention and control of fusarium graminearum
  • Bacillus tequilensis and its use in prevention and control of fusarium graminearum

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Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1, isolation and identification of bacterial strain

[0033] 1. Isolation of strains

[0034] (1) In June 2012, 10 ears of diseased wheat ears collected in Jiangsu were placed in 100 ml sterile distilled water for 15 minutes to prepare a bacterial suspension in an ultra-clean workbench, and then treated in a water bath at 80°C for 10 minutes.

[0035] (2) Dilute the bacterial suspension in a concentration gradient with sterile distilled water, spread it on the NA medium plate, and cultivate it at 30°C for 24 hours. The colonies cover the entire plate. Use an inoculation loop to pick out the shape, size, The strains with different colors and transparency were purified by streaking on the plate, and the purified strains were applied to the confrontation culture experiment of Fusarium graminearum. The results are as follows: figure 1 shown. The obtained strain with strong antagonistic ability to Fusarium graminearum was named JS5L.

[0036] 2. Identification ...

Embodiment 2

[0048] Embodiment 2, the antagonism of Bacillus tequila JS5L to Fusarium graminearum

[0049] The antagonism of Bacillus tequila JS5L to Fusarium graminearum was detected by confrontation culture method.

[0050] 1. Use a hole puncher with a diameter of 5 mm to pick out the bacterial plate at the edge of the cultivated pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG1 colony, and pick the bacterial plate into the center of the PDA plate with a sterilized toothpick.

[0051] 2. Inoculate the same volume and concentration of JS5L on a four-dot dash line at a distance of 25mm from the center of the PDA plate. Take the PDA plate inoculated only with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is antibacterial effect bands formed. Do three parallels for each group. The results are shown in Table 1. It shows that JS5L has a strong inhibitory effect on Fusarium graminearum.

[0052] Table 1 The in...

Embodiment 3

[0055] Embodiment 3, the inhibitory effect of JS5L fermented liquid to Fusarium graminearum

[0056] The antagonism of Bacillus tequila JS5L to Fusarium graminearum was detected by confrontation culture method.

[0057] 1. Use a hole puncher with a diameter of 5 mm to pick out the bacterial plate at the edge of the cultivated pathogenic fungus Fusarium graminearum (Fusarium graminearum) FG1 colony, and pick the bacterial plate into the center of the PDA plate with a sterilized toothpick.

[0058] 2. Inoculate JS5L with the same volume and concentration at four points 25mm away from the center of the PDA plate. Take the PDA plate inoculated only with the plate of Fusarium graminearum as the control group, culture at 28°C for 4-5 days, and observe whether there is antibacterial effect bands formed. Do three parallels for each group. The results are shown in Table 1. It shows that JS5L has a strong inhibitory effect on Fusarium graminearum.

[0059] Table 2 The inhibitory eff...

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Abstract

The invention discloses Bacillus tequilensis and its use in prevention and control of fusarium graminearum. The Bacillus tequilensis has a preservation number of CGMCC No.9499. The Bacillus tequilensis can be used as a material for biological prevention and control of wheat scab and has good application prospect in development of both novel biopesticide and biological prevention and control inocula.

Description

technical field [0001] The invention relates to bacillus tequila, in particular to a bacillus tequila and its application in preventing and treating Fusarium graminearum. Background technique [0002] Fusarium graminearum (Fusarium graminearum) is one of the important pathogens of gramineous crops, which can cause wheat head blight (Fusarium Head Blight, FHB). Fusarium graminearum is a major factor limiting wheat yield (Dubin, 1997) . my country's climatic conditions are very favorable for the occurrence of wheat head blight, and the perennial disease in the middle and lower reaches of the Yangtze River caused a yield loss of 10%-15%, and a production reduction of nearly 50% in a pandemic year (Huang Chang et al., 2000). Due to the influence of global warming and changes in farming systems such as straw returning to the field and no-tillage cultivation, the occurrence area of ​​wheat head blight in my country has rapidly expanded from the Yangtze River Basin to Northwest an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
CPCA01N63/10C12N1/205C12R2001/07
Inventor 赵月菊刘阳邢福国周露王龑
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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