Reagent for detecting tumor marker 5-hydroxyindoleacetic acid in urine and preparation method thereof

A tumor marker, oxindole acetic acid technology, is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of low detection accuracy and achieve the detection process. Simple, universal, and specific effects

Active Publication Date: 2015-03-04
无锡先君生物医药有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The invention provides a reagent for detecting tumor marker 5-hydroxyindoleacetic acid in urine and a preparation method thereof to solve the problem of low detection accuracy

Method used

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  • Reagent for detecting tumor marker 5-hydroxyindoleacetic acid in urine and preparation method thereof
  • Reagent for detecting tumor marker 5-hydroxyindoleacetic acid in urine and preparation method thereof
  • Reagent for detecting tumor marker 5-hydroxyindoleacetic acid in urine and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The tumor marker 5-hydroxyindoleacetic acid detection reagent in the urine includes Hg 2+ , Hg 2 2+ 、Cu 2+ 、WO 4 2- , SO 4 2- and NO 3 - Aqueous solution, where Hg 2+ The concentration is 0.30mol / L, Hg 2 2+ The concentration is 0.40mol / L, Cu 2+ The concentration is 3.03mmol / L, WO 4 2- The concentration is 0.91mmol / L.

[0026] The preparation process is as follows:

[0027] 1) Dilute 1 part of commercially available concentrated sulfuric acid into 3 parts with water, cool to room temperature for later use;

[0028] 2) Dilute 1 part of commercially available concentrated nitric acid into 3 parts with water, and cool to room temperature for later use;

[0029] 3) Preparation of A solution: take a certain amount of mercuric sulfate and dissolve it in the nitric acid solution of step 2), wherein the mercury ion content is 2mol / L;

[0030] 4) Preparation of solution B: take a certain amount of mercurous nitrate and dissolve it in the nitric acid solution of s...

Embodiment 2

[0035] The tumor marker 5-hydroxyindoleacetic acid detection reagent in the urine includes Hg 2+ , Hg 2 2+ 、Cu 2+ 、WO 4 2- , SO 4 2- and NO 3 - Aqueous solution, where Hg 2+ The concentration is 0.30mol / L, Hg 2 2+ The concentration is 0.40mol / L, Cu 2+ The concentration is 3.03mmol / L, WO 4 2- The concentration is 0.91mmol / L.

[0036] The preparation process is as follows:

[0037] 1) Dilute 1 part of commercially available concentrated sulfuric acid into 4 parts with water, cool to room temperature for later use;

[0038] 2) Dilute 1 part of commercially available concentrated nitric acid into 4 parts with water, cool to room temperature for later use;

[0039] 3) Preparation of A solution: take a certain amount of mercury sulfate and dissolve it in the nitric acid solution of step 2), wherein the mercury ion content is 1.6mol / L;

[0040] 4) Preparation of solution B: take a certain amount of mercurous nitrate and dissolve it in the nitric acid solution of step...

Embodiment 3

[0045] The tumor marker 5-hydroxyindoleacetic acid detection reagent in the urine includes Hg 2+ , Hg 2 2+ 、Cu 2+ 、WO 4 2- , SO 4 2- and NO 3 - Aqueous solution, where Hg 2+ The concentration is 0.39mol / L, Hg 2 2+ The concentration is 0.79mol / L, Cu 2+ The concentration is 0.70mmol / L, WO 4 2- The concentration is 4.74mmol / L.

[0046] The preparation process is as follows:

[0047] 1) Dilute 1 part of commercially available concentrated sulfuric acid into 6 parts with water, cool to room temperature for later use;

[0048] 2) Dilute 1 part of commercially available concentrated nitric acid into 6 parts with water, cool to room temperature for later use;

[0049] 3) Preparation of A solution: take a certain amount of mercury sulfate and dissolve it in the nitric acid solution of step 2), wherein the mercury ion content is 2.2mol / L;

[0050] 4) Preparation of solution B: take a certain amount of mercurous nitrate and dissolve it in the nitric acid solution of step...

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Abstract

The invention belongs to urine detection reagents and particularly relates to a reagent for detecting an abnormal metabolite, namely 5-hydroxyindoleacetic acid, in a human body and a preparation method of the reagent. The reagent for detecting a tumor marker, namely 5-hydroxyindoleacetic acid, in urine comprises aqueous solutions of Hg<2+>, Hg2<2+>, Cu<2+>, WO4<2->, SO4<2-> and NO3<->. The preparation method comprises the following steps: preparing a sulfuric acid solution; preparing a nitric acid solution; preparing a solution A; preparing a solution B; preparing a solution C; preparing a solution D; preparing a solution E; mixing. According to the technical scheme provided by the invention, raw materials are easily obtained and are cheap, the preparation process is simple, the obtained reagent is stable in performance and has the characteristics of strong universality, high sensitivity, good specificity and the like when the reagent is applied to cancer detection, and the detection is simple in process, short in time and easy in judgment, so that the reagent is particularly applicable to the massive population check and the clinical auxiliary diagnosis and dynamic treatment effect check of cancers.

Description

technical field [0001] The invention belongs to urine detection reagents, in particular to a detection reagent for abnormal metabolite in human body—5-hydroxyindoleacetic acid and a preparation method. Background technique [0002] Cancer mortality ranks first among all diseases and is the number one killer of human health. It has become a major cause of death that threatens human life and health. According to statistics, the number of cancer patients will double every 25 years. Not only that, the number of deaths from cancer is also rising rapidly around the world, and this number may increase to 13.2 million in 2030. Therefore, the prevention and treatment of cancer has become one of the priorities of the global health strategy, and the key to the treatment of cancer lies in early detection, early diagnosis and early treatment. Imaging detection can often only detect advanced tumor diseases, and it is difficult to achieve the purpose of early detection, and it is expensiv...

Claims

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Application Information

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IPC IPC(8): G01N21/82
CPCG01N21/82
Inventor 刘东黎磊郑明彬
Owner 无锡先君生物医药有限公司
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