Artificial bear gall powder and preparation method thereof

Abstract

The invention discloses artificial bear gall powder and a preparation method thereof. The preparation method comprises the following steps: step (a) dissolving livestock gall powder by using a buffer solution of which the pH value is equal to 7-9 and then filtering to obtain filter residues and a filtrate; step (b) adding the filtrate into a reactor filled with 7alpha-hydroxyl steroid dehydrogenase, 7beta-hydroxyl steroid dehydrogenase and coenzyme I or coenzyme II and reacting; step (c) after the reaction ends, freeze-drying a reaction liquid and then mixing the freeze-dried reaction liquid with the filter residues to obtain the artificial bear gall powder. According to the preparation method disclosed by the invention, completely natural livestock gall is taken as a raw material for preparing the artificial bear gall, no other chemical substances are added, common animal gall is adopted and a gall source is easy to pick, obtain and process; through an in-vitro enterohepatic circulation simulation process, the livestock gall is biologically transformed into the artificial bear gall powder which is equivalent to natural bear gall powder in chemistry, and the artificial bear gall powder is used as a natural bear gall replacement to protect the endangered animal Asian black bear and promote healthy development of the bear gall industry.

Description

technical field [0001] The invention belongs to the field of medicines, in particular to an artificial bear bile powder and a preparation method thereof. Background technique [0002] Bear bile, obtained from the dried gallbladder bile of black bears or brown bears, has the effects of clearing away heat, calming the liver, and improving eyesight. Its main component is tauroursodeoxycholic acid (TUDCA). Bear bile is one of the four famous and precious animal medicines. It has a history of more than 2,000 years of use, and a large number of prescriptions contain bear bile. my country's annual output of bear bile powder is about 30 tons, which still cannot meet the needs of human health. At present, the contradiction between supply and demand of high-end bear bile powder is increasingly prominent and the market demand for bear bile powder for low-end raw materials continues to increase, which makes the price of bear bile powder rise steadily, and the market price has exceeded ...

AI Technical Summary

Problems solved by technology

[0012] There are still problems with the artificial bear bile prepared by the current technology. In some technologies, the content of tauroursodeoxycholic acid in products made by simply mixing different poultry bile cannot reach the standard of the Pharmacopoeia at all.

Many technologies add some chemical substances such as tauroursodeoxycholic acid, bile pigment, amino acid, etc. to the mixture of poultry bile so that the chemical composition of the finished product is similar to that of natural bear bile powder, but it cannot be truly Become a bear bile substitute

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