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Fusarium oxysporum single spore isolation method for soybean root rot

A technology for soybean root rot and Fusarium oxysporum, applied in the field of fungal monospore strain separation, can solve the problems of small Fusarium oxysporum conidia, low probability of monospore strain, poor separation effect, etc., and achieves simple equipment and high accuracy , fast effect

Inactive Publication Date: 2015-02-18
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Although it has been used for many years, it also has its shortcomings: these methods have a good effect on the separation of megaspores, and the conidia of Fusarium oxysporum are small and colorless, and the separation effect of the above methods is poor and difficult; at the same time, these methods are in the process of implementation. The probability of obtaining a single spore is small, the workload is heavy, and the time is long
In particular, the prior art of single spore isolation for soybean root rot fungus Fusarium oxysporum conidia has never appeared, nor has there been any report in this regard

Method used

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  • Fusarium oxysporum single spore isolation method for soybean root rot
  • Fusarium oxysporum single spore isolation method for soybean root rot
  • Fusarium oxysporum single spore isolation method for soybean root rot

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1, the mensuration of the pathogenicity of soybean Fusarium oxysporum monospore strain

[0028] The bacterial strain used in this embodiment is Fusarium oxysporum (Xing An, Wen Jingzhi, Lu Guozhong, Sun Xiaodong. Isolation and identification of Fusarium on soybean root rot diseased strains in Heilongjiang Province, Journal of Northeast Agricultural University, 2009, 40 (8) : 5-9; Wang Jiansheng, Wang Jiamei, Li Xiao, Dong Shameng, Zhang Zhengguang, Zheng Xiaobo. Rapid molecular detection of Fusarium oxysporum. Acta Phytopathology, 2013, 43( 3): 318-322 ), using the above method to isolate single-spore strains of the same Fusarium oxysporum, and obtain 15 single-spore strains, and then isolate the second-generation single-spore strain of No. 12 single-spore strain to obtain 15 second-generation single-spore strains. The soybean varieties used in this embodiment are Heinong 53 and Dongnong 54 purchased from the market.

[0029] The experimental method is as fo...

Embodiment 2

[0050] Embodiment 2, the measurement of different soybean varieties to Fusarium oxysporum disease resistance

[0051] The 14 soybean varieties used in this example are commonly used varieties in production, and the pathogenic bacteria are Fusarium oxysporum monospores isolated from different Fusarium oxysporum (randomly selected soybean root rot pathogen Fusarium oxysporum).

[0052] Use 12 randomly selected Fusarium oxysporum strains to isolate single spores according to the above method, then interact with 14 soybean varieties, and measure the disease resistance of 14 soybean varieties according to the method described in Example 1.

[0053] The experimental results are shown in Table 3.

[0054] Table 3 Resistance performance of different varieties to Fusarium oxysporum strains

[0055]

[0056] As can be seen from Table 3, the results of the interaction between 12 randomly selected single-spore strains of Fusarium oxysporum and 13 varieties showed that there were signi...

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Abstract

The invention discloses a fusarium oxysporum single spore isolation method for the soybean root rot. The fusarium oxysporum single spore isolation method is provided for solving the problems that a soybean fusarium oxysporum single spore is small, is in light color and is not easy to isolate. The fusarium oxysporum single spore is enlarged in size after germinating, then dilution is carried out until a spore suspension with only 1-2 spores in average every 10 <Mu>L is obtained, and isolated conidium is further confirmed in a round hole grid in a 96-mesh cell culture plate cover through an inverted microscope to be a single spore. Compared with other single spore isolation methods, the method is good in operability, high in accuracy, high in speed, excellent in isolation effect, and simple in equipment. The method is accurate, simple and feasible, and a large batch of fusarium oxysporum single spores can be isolated within a short period of time in a lab. Therefore, the method is suitable for promotion and application.

Description

technical field [0001] The invention relates to a method for isolating single spores of fungi, in particular to a method for isolating single spores of Fusarium oxysporum conidia of soybean root rot. Background technique [0002] Fusarium soybean root rot (soybean Fusarium root rot) is produced by Fusarium oxysporum ( F. oxysporum Schl.) is caused by the infection of dominant strains, and is a worldwide disease with wide distribution, serious damage and difficult control. The longer the soybean planting years, the more severe the disease and the lower the yield. Areas with normal incidence of soybean root rot can reduce soybean production by 10%-30%, and areas with severe diseases can reduce production by 60%, or even stop production. Soybean root rot mainly harms the root system of the plant, thereby affecting the absorption of water and nutrients by the root system, resulting in retarded plant growth and reduced yield. Soybean breeding for disease resistance is the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N3/00C12R1/77
CPCC12N1/14C12N3/00
Inventor 李永刚李文彬潘春清王春玲
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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