Method and apparatus for preparing single donor thrombin serum

Abstract

A method for preparing thrombin serum, the method comprising obtaining blood fluid sample, contacting a first aliquot of the blood fluid with a procoagulant agent to form prothrombinase enzyme complex bound to the surface of the procoagulant agent so as to obtain an activated procoagulant agent that may be stored. The activated procoagulant agent may then be contacted with a second aliquot of the blood fluid containing prothrombin so as to obtain thrombin serum, which may be extracted and contacted with fibrinogen to obtain fibrin.

Description

[0001] Related application [0002] This patent application is a non-provisional application of U.S. Provisional Patent Application 61 / 705,535 filed on September 25, 2012, and claims the priority of the patent application. The entire content of the patent application is incorporated herein to form an integrated document. Technical field [0003] The present invention relates to the conversion of prothrombin in blood to thrombin, and in particular to a device and method for increasing the concentration of activated cascade coagulation protein in blood. Background technique [0004] Plasma coagulation is considered to be through a series of self-amplifying zymogen-enzyme conversion ( figure 1 ) Occurs, in the penultimate step of this conversion process thrombin (FIIa) is produced, which is a powerful serine protease. In the final step called the "coagulation cascade", prothrombin is converted into thrombin by a calpain-containing complex called prothrombinase complex. Thrombin is an ...

AI Technical Summary

Problems solved by technology

[0028] Despite these advances, an ideal method for preparing single-donor thrombin serum in the healthcare setting is still lacking

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