Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

mir-223 complex targeting microglial cells and its preparation method and application

A technology of microglia and mir-223, applied in the field of biomedicine, can solve the problems of non-breakthrough treatment methods, achieve the effects of inhibiting inflammatory reactions, increasing uptake rate, and enhancing transfection efficiency

Inactive Publication Date: 2017-09-01
AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the medical treatment of cerebral hemorrhage is mainly based on dehydration to reduce intracranial pressure, scavenging free radicals, nourishing nerves and symptomatic and supportive treatment. In recent years, there is no breakthrough treatment method

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • mir-223 complex targeting microglial cells and its preparation method and application
  • mir-223 complex targeting microglial cells and its preparation method and application
  • mir-223 complex targeting microglial cells and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Preparation of miR-223 complex targeting microglia

[0022] 1. Preparation of T9-V9 fusion peptide

[0023] The H9-V9 fusion peptide is formed by directly connecting the carboxyl terminal of the H9 peptide with the amino terminal of the V9 peptide, wherein the amino acid sequence of the H9 peptide is HHHHHHHHH (SEQ ID No.1), and the amino acid sequence of the V9 peptide is LTQQVVMKF (SEQ ID No. 2) The amino acid sequence of the formed H9-V9 fusion peptide is HHHHHHHHH-LTQQVVMKF (SEQ ID No.3).

[0024] The synthesis of the H9-V9 fusion peptide was carried out on an AB-431A peptide synthesizer using a standard Fmoc protocol. Using 0.25mmol p-hydroxymethylphenoxymethyl polystyrene (HMP) resin as the starting resin, according to the amino acid sequence of the H9-V9 fusion peptide, the peptide chain is extended from the carboxyl terminal to the amino terminal one by one. After the peptide chain is synthesized, , transfer the resin containing the peptide chain to...

Embodiment 2

[0030] Example 2. Research on anti-inflammatory effect of miR-223 complex targeting microglia

[0031] 1. Detection of inflammatory factors in microglia after red blood cell lysate treatment

[0032]Microglial cells were cultured in a monolayer in a 6-well plate to about 30% of the coverage area, replaced with 2 mL of serum-free DMEM medium, and 100 μL of miR-223 complex was added (after the addition, the final concentration of miR-223 complex was 1 μg / mL), cultured for 4 hours, then replaced with 2 mL of complete DMEM medium, cultured for 48 hours, collected cells, washed with PBS and resuspended to obtain miR-223 complex transfected microglial cells. At the same time, the negative control complex was added in the same way as the control microglia. Add 10 μL of erythrocyte lysate to microglia or control microglia, and then incubate at 37°C for 24 hours. ELISA is used to detect the ability of erythrocyte lysate to stimulate microglia to secrete IL-8 and TNF-α. The result is...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a miR-223 compound of targeted microglia, as well as a preparation method and application thereof. The miR-223 compound of the targeted microglia is formed by combining H9-V9 fusogenic peptide and miR-223 by means of the electrostatic interaction, wherein the H9-V9 fusogenic peptide is formed by connecting the carboxyl terminal of H9 peptide and the amino terminal of V9 peptide. According to the compound, a specific MCP-1 receptor on the surface of the targeted microglia is specified by utilizing the guiding action of the V9 peptide, while the V9 peptide and MCP-1 receptor act, the miR-223 is effectively transfected into the microglia, activation of the microglia is inhibited, and the microglia mediated inflammation response can be inhibited. The miR-223 compound can be used for preparing medicines for treating microglia inflammatory injury after cerebral hemorrhage, and has an excellent development application prospect in the field of cerebral hemorrhage treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a miR-223 complex targeting microglial cells, and also relates to a preparation method and application of the complex. Background technique [0002] Cerebral hemorrhage is a common critical illness of the nervous system, which has attracted widespread attention due to its high morbidity, disability and mortality. With the improvement of people's living standards and the arrival of aging, the incidence of cerebral hemorrhage is also on the rise. According to the latest statistical data, the number of patients who die from cerebral hemorrhage every year is about 20% of the number of patients who die from all diseases. At present, the medical treatment of cerebral hemorrhage is mainly based on dehydration to reduce intracranial pressure, scavenging free radicals, nourishing nerves and symptomatic and supportive treatment. In recent years, there is no breakthrough treatment me...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/00A61K47/68A61P29/00
Inventor 杨曌
Owner AFFILIATED YONGCHUAN HOSPITAL OF CHONGQING MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com