Bioslurry repair method for poly brominated diphenyl ethers polluted soil, and special equipment

A polybrominated diphenyl ether and polluted soil technology, which is applied in the field of biological mud restoration of polybrominated diphenyl ether polluted soil, can solve problems such as weakened thyroid function, and achieve the effects of shortening the degradation time and improving the degradation effect.

Inactive Publication Date: 2015-01-28
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The study of the impact on the human body found that the thyroid function of workers engaged in the production of PBDEs was significantly weakened

Method used

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  • Bioslurry repair method for poly brominated diphenyl ethers polluted soil, and special equipment
  • Bioslurry repair method for poly brominated diphenyl ethers polluted soil, and special equipment
  • Bioslurry repair method for poly brominated diphenyl ethers polluted soil, and special equipment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Step 1): In this example, a bioslurry reactor made of stainless steel with a volume of 50L and equipped with stirring and aeration devices was used for the test; the seed tank for cultivating fungi and bacteria was a 15L fermenter with stirring and aeration devices ; The fungi used in this example are 1 strain of Phanerochaete chrysosporium, and the bacteria are 1 strain of Sphingomonas; the Sphingomonas that will be cultivated in the LB liquid medium is inoculated with 2% inoculum Carry out expanded cultivation in the bacterial seed tank; Simultaneously the Phanerochaete chrysosporium cultivated in the potato liquid medium is inoculated into the fungal seed tank with the amount of 2%; In the present embodiment, dress 30L polybrominated diphenyl ether contaminated soil, water and soil in the biological reaction The ratio is 3:1. Add inorganic salt nutrients to the bio-slurry reactor to adjust the content of inorganic salts in the reactor: Na 2 HPO 4 12H 2 o 7 .7.036...

Embodiment 2

[0039] Embodiment 2: polybrominated diphenyl ether polluted soil and water are 2:1 with water-to-soil volume ratio, adjust the inorganic salt content in the reactor: Na 2 HPO 4 12H 2 O6g / L, KH 2 PO 4 0.8g / L, (NH 4 ) 2 SO 4 0.3g / L, MgCl 2 ·6H 2 O 0.08g / L, Ca(NO 3 ) 2 4H 2 O40mg / L; trace element content FeSO 4 ·7H 2 O 2g / L, ZnSO 4 ·7H 2 O 0.02g / L,H 3 BO 3 0.2g / L, CoCl 3 ·6H 2 O0.2g / L, NiCl 2 ·6H 2 O0.02g / L,Na 2 MoO 4 2H 2 O 0.02g / L; Inoculate fungi and bacteria with polybrominated diphenyl ether degradation ability while reacting, the inoculum size is 5%; At a temperature of 25 ° C, the ventilation rate is 0.002m 3 / s, other with embodiment 1.

Embodiment 3

[0040] Embodiment 3: polybrominated diphenyl ether polluted soil and water are 4:1 with water-to-soil volume ratio, the inorganic salt content in the adjustment reactor: Na 2 HPO 4 12H 2 O 8g / L, KH 2 PO 4 1.2g / L, (NH 4 ) 2 SO 4 0.8g / L, MgCl 2 ·6H 2 O 0.12g / L,

[0041] Ca(NO 3 ) 2 4H 2 O460mg / L; trace element content FeSO 4 ·7H 2 O 3g / L, ZnSO 4 ·7H 2 O 0.04g / L, H 3 BO 3 0.4g / L, CoCl 3 ·6H 2 O0.3g / L, NiCl 2 ·6H 2 O 0.03g / L,Na 2 MoO 4 2H 2 O 0.04g / L, the inoculum size is 15%; the temperature is 30°C, and the ventilation volume is 0.008m 3 / s condition, others are the same as embodiment 1.

[0042] The microorganisms capable of degrading PBDEs described in the present invention can be obtained by screening PBDE-contaminated soils, or obtaining known high-efficiency degrading strains from bacterial strain preservation centers and research institutions in various countries. The fungi and bacteria inoculated in the bioreactor are first amplified and cultured...

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Abstract

The invention relates to a bioslurry repair method for poly brominated diphenyl ethers polluted soil, and special equipment, and belongs to the technical field of environment protection. The bioslurry repair method is characterized by comprising the following steps of (1) adding the poly brominated diphenyl ethers polluted soil and water mixedly into a bioreactor, adding inorganic salt nutrients and microelements and inoculating fungi and bacteria during reaction, wherein an inoculation amount is 5%-15%, and a rotation speed of stirring paddles of the reactor is controlled at 110-150 rpm when a temperature is 25-30 DEG C and an aeration rate is 0.002-0.008 m<3>/s; and (2) firstly determining a brominated number polluted by poly brominated diphenyl ethers in the polluted soil, then regulating the inoculating ratio of the fungi to bacteria to increase a degradation effect and shorten a degradation time. The above bioslurry repair method for the poly brominated diphenyl ethers polluted soil is simple to operate, has strong capacity for removing poly brominated diphenyl ethers, and has a good repairing effect.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a bio-slurry restoration method for polybrominated diphenyl ether-contaminated soil. Background technique [0002] The English name of polybrominated diphenyl ethers is Poly Brominated Diphenyl Ethers (referred to as PBDEs), and there are 209 kinds of homologues such as tetrabromodiphenyl ether, pentabromine, hexabromine, octabromine, and decabromine. Its commercial polybrominated diphenyl ethers are a group of diphenyl ether mixtures with different numbers of bromine atoms, so they are collectively called polybrominated diphenyl ethers. Polybrominated diphenyl ethers (PBDEs) are a class of global organic pollutants that have attracted much attention due to their persistence, toxicity and potential bioaccumulation. PBDEs are a class of global organic pollutants that widely exist in the environment. Because of its environmental persistence, long-dista...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10
Inventor 张昕赵宇华杜敬霆童森淼
Owner ZHEJIANG FORESTRY UNIVERSITY
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