Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Lipid and growth trait genes

A lipid and lipophilic technology, applied in genetic engineering, plant genetic improvement, microorganisms, etc., can solve the problems of lack and difficulty in using microalgae

Inactive Publication Date: 2015-01-21
SAPPHIRE ENERGY
View PDF22 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the slowed growth of microalgae under nitrogen starvation conditions makes it more difficult to use microalgae in large-scale production of biofuels
Although algae provide natural feedstocks in the form of lipid-rich feedstocks, we lack an understanding of the details of lipid metabolism used to manipulate processes physiologically and genetically

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lipid and growth trait genes
  • Lipid and growth trait genes
  • Lipid and growth trait genes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0666] Example 1: Nitrogen starvation phenotype in wild-type algae.

[0667] Nitrogen starvation is known to cause several phenotypes in many wild-type algal species (e.g., Dunaliella salina, Scenedesmus dimorphus, Dunaliella viridans, Chlamydomonas reinhardtii, and Nannochloropsis), including total lipid Quality increase type ( Figure 8A and 8B , Figure 41C ), reduced growth rate ( Figure 8C , Figure 41A and 41D ), and the decomposition type of chlorophyll ( Figure 8D and Figure 41B and 41E ). It is desirable to isolate these phenotypic pathways at the molecular level. For example, it is desirable to obtain an enhanced lipid phenotype without reduced growth rate and decomposed algal components.

[0668] Figure 8A Gravity fat analysis (hexane extractables) is shown. The left-hand columns of each of the two groups are in NH containing 7.5mM 4 Percent hexane-extractable lipids (%DW) after growth in medium in Cl, and in the right-hand column for each of the tw...

Embodiment 2

[0677] Example 2: Chronology of Stress Responses in Wild-Type Chlamydomonas reinhardtii at the Biochemical and Molecular Levels

[0678] In this example, the timing of biochemical and molecular reactions of wild-type Chlamydomonas reinhardtii was studied. Wild-type C. reinhardtii cells were grown in constant light in an air environment containing 5-10 L of HSM, 5% CO2, until the cells reached early logarithmic phase. The culture broth was centrifuged at 3000-5000xg for 5-10 minutes, half of the culture broth was washed with 500-1000 mL of HSM, and the other half of the culture liquid was washed with 500-1000 mL of HSM not containing nitrogen. After centrifugation again, both cultures were resuspended in a volume of medium (HSM or HSM without nitrogen) corresponding to the starting medium volume. At the time points listed in Table 2, 0.5-2 L of cells were harvested by centrifugation and analyzed for total lipid weight according to the BlighDyer method (eg BLIGH and DYER, A rap...

Embodiment 3

[0687] Embodiment 3: the method of transcriptome sequencing transcriptomics

[0688]In this example, an exemplary method for the identification of the gene encoding SN03 is described. The methods described here can be used to identify other proteins, polypeptides, or transcription factors, for example, those observed in organisms (eg, algae) that are involved in regulating or controlling different nitrogen deficiency phenotypes. These nitrogen-deficient phenotypes include, for example, increased lipid production and / or accumulation, breakdown of the photosynthetic system, reduced growth, and induction of hybridization. Genes identified as involved in the regulation or control of different phenotypes of nitrogen deficiency may have a positive or negative effect on these phenotypes, for example, increasing or decreasing lipid production, or increasing or decreasing growth rate.

[0689] In order to identify genes / proteins involved in nitrogen starvation-induced lipid phenotyp...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present disclosure provides novel lipid and growth stress response target genes isolated from Chlamydomonas reinhardtii that when over expressed in an organism results in a change in the lipid profile, and / or lipid content, and / or growth of the organism. The present disclosure also describes organisms expressing the genes, and methods of using the novel genes to change the lipid content, lipid profile or growth of an organism.

Description

[0001] Cross References to Related Applications [0002] This application claims priority from US Provisional Patent Application No. 61 / 602,892, filed February 24, 2012, which is hereby incorporated by reference in its entirety for all purposes. Background technique [0003] Microalgae represent a diverse group of microorganisms adapted to various ecological habitats (eg, as in Hu et al., Plant J. (2008) pp. 54(4) 621-639). Many microalgae are capable of producing large amounts (eg, 20-50% of dry cell weight) of lipids, such as triacylglycerols (TAG) and diacylglycerols (DAG), as storage lipids under stressful conditions, such as nitrogen starvation. Under nitrogen starvation conditions, many microalgae exhibit reduced growth rates and breakdown of components of photosynthesis, such as chlorophyll. [0004] Fatty acids, the building blocks for TAG and all other cellular lipids, are synthesized in chloroplasts using a single set of enzymes, with acetyl-CoA carboxylase (ACCase)...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/31C12R1/89
CPCC07K14/405C12N9/00C12P7/6463C12N15/8247
Inventor C·约恩
Owner SAPPHIRE ENERGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com