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Epitope minimum motif peptide of P1, VP2 and VP4 structural proteins in type O foot and mouth disease virus (FMDV) strain (O/BY/CHA/2010) and application of epitope minimum motif peptide

A technology of foot-and-mouth disease virus and structural protein, applied in the field of biomedicine and biological detection

Active Publication Date: 2015-01-21
FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since then, there have also been identifications of epitopes of the Asial type I coat protein, VP1 (1-12aa), (17-29aa), (194-211aa); VP2 (40-50aa), VP3 (26-39aa), VP4 (30-41), but the fine epitope identification has not been carried out (Zhang ZW 1 ,Zhang YG,Wang YL,Pan L,Fang YZ,Jiang ST,Lü JL,Zhou P.Screening and identification of B cell epitopes of structural proteins of foot-and-mouth disease virus serotype Asia1.Vet Microbiol.2010 Jan6;140 (1-2):25-33.)

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  • Epitope minimum motif peptide of P1, VP2 and VP4 structural proteins in type O foot and mouth disease virus (FMDV) strain (O/BY/CHA/2010) and application of epitope minimum motif peptide
  • Epitope minimum motif peptide of P1, VP2 and VP4 structural proteins in type O foot and mouth disease virus (FMDV) strain (O/BY/CHA/2010) and application of epitope minimum motif peptide
  • Epitope minimum motif peptide of P1, VP2 and VP4 structural proteins in type O foot and mouth disease virus (FMDV) strain (O/BY/CHA/2010) and application of epitope minimum motif peptide

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Embodiment 1

[0033] This embodiment is the identification of FMDV type O strain O / BY / CHA / 20104 structural protein fine epitopes:

[0034] (1) Design of target protein series 18 / 8 polypeptide-encoding DNA fragments

[0035] According to the gene sequence of the three structural proteins VP1, VP2, and VP4 of FMDV O strain O / BY / CHA / 2010 in GenBank (GenBank accession number JN998085.1), after codon optimization of Escherichia coli, the design covers three The full-length sequences of each protein gene are 18 / 8 polypeptide-encoded DNA fragments overlapping 10 aa residues, and BamH I and TAA-Sal I cohesive end sequences are added to the two ends of the fragments, and the short peptide-encoded DNA is chemically synthesized. Negative strand fragment. After completing the first round of 18-mer peptide scanning and mapping, a series of 8-mer peptides overlapping 7 residues were designed for the reactive peptides, and at the same time targeting the previously discovered antigenic epitopes VP1(21-40)...

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Abstract

The invention belongs to the technical field of biological medicine and biological detection and particularly relates to an epitope minimum motif peptide of P1, VP2 and VP4 structural proteins in a type O foot and mouth disease virus (FMDV) strain (O / BY / CHA / 2010) and application of the epitope. Five linear epitope minimum motif peptides provided by the invention can be used as candidate epitope peptides for research and development of a novel recombinant multi-epitope peptide vaccine against type O FMDV and have amino acid sequences as shown in SEQ. ID NO.1-SEQ. ID NO.5. The invention also provides a candidate epitope for research of a detection antigen of a high-specificity and high-sensitivity recombinant multi-epitope peptide for diagnosing prevalence of the FMDV and the candidate epitope has amino acid sequences as shown in the SEQ. ID NO.6-SEQ. ID NO.10.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and biological detection, and specifically relates to epitope minimum motif peptides, extended peptides and peptides of structural proteins VP1, VP2 and VP4 in O-type foot-and-mouth disease virus (FMDV) strain (O / BY / CHA / 2010). its application. Background technique [0002] Foot-and-mouth disease of livestock is the most serious livestock infectious disease in the world today, mainly harming pigs, cattle, sheep and other cloven-hoofed animals. For many years, foot-and-mouth disease has broken out and become popular in a large scale all over the world, causing huge economic losses to animal husbandry. According to immunogenicity, there are 7 serotypes and more than 65 subtypes of FMD virus: A, O, C, SAT Ⅰ, SAT Ⅱ, SAT Ⅱ and Asia I. [0003] Immunization is the main way to prevent foot-and-mouth disease outbreaks in developing countries. Traditional inactivated vaccines have the advantages of h...

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61K39/135A61P31/14G01N33/569
Inventor 刘明秋郑兆鑫栾喜梅
Owner FUDAN UNIV
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