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Application of GFP-30 protein and lipidosome in preparation protein medicine used for treating sound sensing nerve deafness and inherited deafness

A GFP-30, 1. GFP-30 technology, applied in the application field of preparing protein drugs for the treatment of sensorineural deafness or hereditary deafness, can solve the problem of low protein transfection efficiency, and achieve the effect of avoiding gene contamination

Inactive Publication Date: 2015-01-21
EYE & ENT HOSPITAL SHANGHAI MEDICAL SCHOOL FUDAN UNIV
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  • Claims
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AI Technical Summary

Problems solved by technology

However, the transfection efficiency of pure protein is extremely low in vivo experiments, which limits its application

Method used

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  • Application of GFP-30 protein and lipidosome in preparation protein medicine used for treating sound sensing nerve deafness and inherited deafness
  • Application of GFP-30 protein and lipidosome in preparation protein medicine used for treating sound sensing nerve deafness and inherited deafness

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Experimental program
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Effect test

Embodiment 1

[0021] 1. Animal grouping and preparation of GFP-30-Cre protein

[0022] Td-Tomato mice were divided into GFP-30-Cre protein and liposome combined group and liposome injection group, with 8 mice in each group. The GFP-30 protein is negatively charged, the Cre protein is positively charged, and the combination of the two is still negatively charged.

[0023] 2. GFP-30-Cre protein and liposome binding

[0024] The original concentration of GFP-3-Cre protein was 225 μM, diluted 5 times with Optimem, mixed with liposome RNA iMax or liposome 2000 at a ratio of 1:1 (volume ratio (v / v), and mixed at room temperature Injections were performed 30 minutes later.

[0025] 3. Microinjection into inner ear

[0026] 1. All surgical instruments are dried after being sterilized by high temperature and high pressure;

[0027] 2. Newborn rats were treated with ice anesthesia. After anesthesia, the operation area was disinfected with 70% alcohol;

[0028] 3. Take the left decubitus position...

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Abstract

The invention belongs to technical field of biological medicines and particularly relates to application of GFP-30 protein and lipidosome in preparation protein medicine used for treating sound sensing nerve deafness and inherited deafness. According to the invention, the medicine manufacturing application provides a safe and efficient method for gene therapy the sound sensing nerve deafness and the inherited deafness.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the application of GFP-30 protein and liposome in the preparation of protein medicine for treating sensorineural deafness or hereditary deafness Background technique [0002] According to the data released by the World Health Organization, there are 250 million patients with moderate and severe deafness in the world. According to the second sample survey of disabled people in my country in 2006, the number of hearing disabled people in my country has reached 27.8 million, and it is still increasing. %. Due to the lack of damage repair and self-regeneration ability of inner ear hair cells and spiral nerves, the research on hair cell regeneration is very important. Recently, it has been reported that inner ear support cells express Atoh1 gene, which can make support cells transdifferentiate into hair cells, but this gene The expression of adenovirus is carried by adenovirus, and ...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K38/16A61P27/16
Inventor 舒易来李华伟陈正一大卫刘约翰祖易斯大卫汤普森
Owner EYE & ENT HOSPITAL SHANGHAI MEDICAL SCHOOL FUDAN UNIV
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