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A rapid screening method for sensitive herbicides of parasitic weeds

A herbicide and sensitivity technology, applied in the field of rapid herbicide screening, can solve the problems of crop environmental damage, indiscriminate use of herbicides, differences in resistance of different crops, etc., to avoid water loss, prevent bacterial contamination, and eliminate false positive results. Effect

Active Publication Date: 2016-02-24
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The disadvantage of this method of weeding is that it lacks systematic and in-depth research. Indiscriminate use and abuse of herbicides often occur in agricultural production, causing significant damage to crops and the environment.
At the same time, the field screening of herbicides often has problems such as long period (at least the entire growth cycle of the crop is required), large investment (requires a large number of crops to be planted in a large area), high risk (potential risk of yield loss), and field screening. There are also often uncertain factors, such as differences in seed density and species of parasitic weeds in different fields, differences in resistance of different crops, etc., which will cause false positive results

Method used

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  • A rapid screening method for sensitive herbicides of parasitic weeds
  • A rapid screening method for sensitive herbicides of parasitic weeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 148

[0041] The control effect test of embodiment 148% Zhongbutaline EC to sunflower column (Orobanchecumana Wallr)

[0042] ⑴Reagent preparation

[0043] ① Disinfectant preparation, in 100ml: 20ml of 5% sodium hypochlorite stock solution plus 30ml sterile distilled water to dilute into 2% sodium hypochlorite solution, 0.1g of Tween-20 plus 49.9ml of sterile distilled

[0044] ②10 -6 M GR24 solution preparation, take 3mg solid GR24 dissolved in acetone and dilute to 10ml to form 10 -3 M's GR24 mother solution, take 10μl of the mother solution and add sterile distilled water to 10ml to make 10 -6 The GR24 solution of M was sterilized by filtration with a 0.22 μm filter membrane;

[0045] ③Preparation of herbicide solution, take 1.2μl 48% Zhongbuling EC dissolved in 1.5ml sterile distilled water to make 600mg / m 2 The herbicide solution was diluted 10 times and 100 times respectively to form 600mg / m 2 、60mg / m 2 、6mg / m 2 Concentration gradient, sterilized by filtration with a 0....

Embodiment 296

[0055] Embodiment 296% Jin Duer EC is to the control effect test of Orobancheminor (Orobancheminor)

[0056] ⑴Reagent preparation:

[0057] ① Disinfectant solution preparation, in 100ml: 20ml of 5% sodium hypochlorite stock solution plus 30ml sterile distilled water to dilute into 2% sodium hypochlorite solution, 0.1g of Tween-20 plus 49.9ml sterile distilled water to make a 0.2% solution, mix the two The solution is mixed to make a disinfectant;

[0058] ②10 -6 M GR24 solution preparation, take 3mg solid GR24 dissolved in acetone and dilute to 10ml to form 10 -3 M's GR24 mother solution, take 10μl of the mother solution and add sterile distilled water to 10ml to make 10 -6 The GR24 solution of M was sterilized by filtration with a 0.22 μm filter membrane;

[0059] ③ Herbicide solution preparation, take 0.73μl 96% Jinduer EC dissolved in 1.5ml sterile distilled water to make 375mg / m 2 The herbicide solution was diluted 10 times and 100 times respectively to form 375mg / m ...

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Abstract

The invention discloses a method for rapid screening of a parasitic weed sensitive herbicide. The method comprises: taking parasitic weed seeds, conducting surface sterilization, rinsing and air drying; inoculating the seeds to a moisture absorption matrix containing a to-be-screened herbicide, and carrying out pre-culture under closed and dark conditions; transferring the seeds to the moisture absorption matrix containing a seed germination stimulus, and conducting germination test under closed and dark conditions; calculating the seed germination rate, and screening out the parasitic weed sensitive herbicide. The herbicide is directly used in the pre-culture stage of the parasitic weed seeds to simulate the field application and action process of the herbicide. The seed germination stimulus GR24 is employed to replace a host root exudate, thereby eliminating a false positive result caused by germination stimulus type and content differences, and making seeds germinate under laboratory conditions. The control effect of the herbicide on seed germination can be quickly definite by only 2 weeks. The data is true and reliable, and provides reference for field use of the herbicide.

Description

technical field [0001] The invention belongs to the technical field of rapid screening of herbicides, in particular to a method for rapid screening of sensitive herbicides of parasitic weeds. Background technique [0002] Parasitic plants refer to a class of plants that must rob certain nutrients and water from the host to meet their own growth and development needs due to root or leaf degeneration or lack of sufficient chlorophyll. [0003] According to the degree of dependence on the host, parasitic plants can be mainly divided into two types: one type has no leaves or the leaves degenerate into scales, and cannot perform normal photosynthesis. The nutrients required by itself come entirely from the host, which is called a total parasitic plant. , such as Cuscuta (Cusutachinensis) and Liedang (Orobanchespp.), etc.; the other type contains chlorophyll and can carry out normal photosynthesis, but the roots are mostly degenerated, and the vessels are directly connected to the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01C1/00
Inventor 杨翀周伟军宋文坚朱金文胡露飏王尖许玲
Owner ZHEJIANG UNIV
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