Fluorescent microsphere-colloidal gold dual-color qualitative and quantitative immunochromatographic test strip for detecting clenbuterol hydrochloride and preparation method thereof

Abstract

The invention discloses a fluorescent microsphere-colloidal gold double color development qualitative quantitative immunochromatographic test strip for detecting clenbuterol hydrochloride and a preparation method thereof. A bottom plate is overlapped with a filter paper, a sample pad, a glass fiber mat sprayed with a fluorescent microsphere-clenbuterol hydrochloride monoclonal antibody complex anda colloidal gold-clenbuterol hydrochloride monoclonal antibody complex, a nitrocellulose membrane and an absorbent paper, and the nitrocellulose membrane is coated with a clenbuterol hydrochloride coupling antigen as a test line and coated with an anti-mouse antibody as a quality control line. If the color development of the detection line is lighter than that of the quality control line, the content of the clenbuterol hydrochloride in a sample is greater than 0.5 ng / mL, and qualitative determination is positive. The test strip qualitatively judged to be positive is directly inserted into a fluorescence reader, and the reader numerically quantifies a fluorescence signal of a fluorescent microsphere color development system to realize quantitative detection. The fluorescent microsphere-colloidal gold double color development qualitative quantitative immunochromatographic test strip for detecting clenbuterol hydrochloride and the preparation method thereof are mainly used for qualitative and quantitative detection of the clenbuterol hydrochloride in food safety testing.

Description

Technical field [0001] The invention belongs to the field of veterinary drug residue detection in food safety, and specifically relates to a time-resolved fluorescent microsphere-colloidal gold double color qualitative and quantitative immunochromatographic test strip that can simultaneously qualitatively and quantitatively detect clenbuterol in a sample and a preparation method thereof. Background technique [0002] Clenbuterol Hydrochloride (CLE) is a synthetic β-adrenergic stimulant, which has the effect of expanding the bronchus. It is commonly used to prevent and treat lung diseases such as asthma and emphysema. But when its applied dose reaches 5-10 times the therapeutic dose, it can increase muscle synthesis and reduce fat deposition, so it is commonly called "Clenbuterol". In animal husbandry production, some illegal users add it as a growth promoter to animal feed to increase the growth rate of lean meat and also cause clenbuterol residues in animal food. When people ea...

AI Technical Summary

Problems solved by technology

[0005] ⑴General test strips are qualitatively analyzed by naked eye observation results, and accurate quantitative detection cannot be achieved;

[0006] (2) The matrix effect of different materials is obvious, and the sample background interference is large, which is easy to produce false positive results;

[0007] (3) The positive result of the test cannot be saved, usually after the judgment time is exceeded, the result is no longer accurate and reliable

[0009] ⑴In the actual test, the proportion of negative results is very high, but all test strips of this method need to be read by an instrument, otherwise the results cannot be obtained, and it takes a long time to test a large number of samples!

[0010] ⑵In quantitative detection, the detection department still needs a threshold to distinguish negative and positive, so the amount of data processing is relatively large;

[0011] (3) The sensitivity of a single fluorescent microsphere test strip is high, but in practical applications, the detection linear range of the higher sensitivity is narrower, and the quantitative concentration range of the sample is not suitable for the actual detection requirements

Images