Simultaneous determination of four banned and restricted additives in tobacco flavors and fragrances by high performance liquid chromatography with fluorescence detection

Abstract

The invention discloses a high performance liquid chromatography fluorescence detection method for simultaneously measuring four prohibited and limited additives in tobacco flavors and perfumes. The high performance liquid chromatography fluorescence detection method comprises the following steps: firstly performing oscillating extraction on the tobacco flavors and perfumes by using alcohol, standing to pass through a 0.45 micrometer organic phase filtering membrane to obtain a to-be-detected sample solution; and simultaneously preparing standard solutions of the four prohibited and limited additives, respectively performing detection analysis on the to-be-detected sample solution and the standard solutions by adopting a high performance liquid chromatography fluorescence detector, and then performing quantitative analysis by adopting an external standard method, figuring out contents of the four prohibited and limited additives in the tobacco flavors and perfumes, wherein the four prohibited and limited additives comprise sesamol, beta-naphthol, beta-asarone and safrole. According to the high performance liquid chromatography fluorescence detection method, simultaneous measurement of the four prohibited and limited additives in the tobacco flavors and perfumes is realized. Because special chromatographic columns for phenols are used, the detection time is relatively short; the fluorescence detector is adopted, the selectivity is relatively high, the matrix interference is relatively small, the detection limit is relatively low; no water removal is needed before treatment, and the relative simplicity is achieved in operation.

Description

technical field [0001] The invention relates to a detection method for four prohibited and restricted additives in tobacco flavors and fragrances, in particular to a high-performance liquid chromatography fluorescence detection method for simultaneously measuring four prohibited and limited additives in tobacco flavors and fragrances. Background technique [0002] The function of adding flavors and fragrances to cigarettes is to improve the physical and chemical properties of cigarettes, round the aroma, modify the flavor of the cigarette, improve the taste, and establish the style of the product. Tobacco flavors and fragrances are a general term for flavoring substances extracted from flavored substances or obtained by artificial synthesis and used in tobacco. Under normal circumstances, tobacco flavors and fragrances are prepared from a variety of flavors, appropriate amount of solvents and other additives in a certain proportion and process. Among the additives, sesamol,...

AI Technical Summary

Problems solved by technology

Since sesamol, β-naphthol, β-asarone and safrole all have strong fluorescence responses, and there is no report on the detection of the above four prohibited and restricted substances in tobacco flavors and fragrances by HPLC-FD

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