Myocardial cell mechanical beating detection method based on optical image analysis

Abstract

The invention discloses a myocardial cell mechanical beating detection method based on optical image analysis. The method comprises the following steps: detecting the change of beating images of myocardial cells in mechanical beating by adopting image treatment and analysis techniques such as image acquisition, RGB (Red, Green, Blue) image-to-gray-image, image binaryzation and image matrixing by virtue of a myocardial cell mechanical beating detection system, and quantizing myocardial cell mechanical beating by adopting a beating image difference value to achieve the detection of a mechanical beating curve of the myocardial cells; and according to the obtained mechanical beating curve of the myocardial cells, calculating the beating rate and amplitude of the myocardial cells by virtue of a peak detection algorithm, and analyzing a mechanical beating state of the myocardial cells. Compared with an existing myocardial cell mechanical beating detection method, the myocardial cell mechanical beating detection method based on optical image analysis disclosed by the invention has the advantages that the method is mark-free and nondestructive, is low in cost and simple in operation step, and can be used for synchronously and directly detecting a cell beating image and analyzing the cell beating state, and the like.

Description

technical field [0001] The invention relates to cell optical image detection and analysis technology, in particular to a method for detecting cardiac muscle cell mechanical beating based on optical image analysis. Background technique [0002] In recent years, it has become a hot topic in the field of cardiology to study changes in the contractile function of cardiomyocytes induced by heart disease or drugs. Rhythmic contraction is the basic feature of cardiomyocytes and the source of power for the whole body's blood circulation system. It is closely related to the basic components of cardiomyocytes, such as microtubules, sarcoplasmic reticulum, and actin, and is an important reflection of cardiac function. In addition, drugs can also cause changes in the contractile function of cardiomyocytes. The detection of cardiomyocyte mechanical beating is of great significance in the field of medicine and drug research. At present, laser confocal microscopy calcium imaging technol...

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Problems solved by technology

Therefore, the operation is complicated and there are limitations in drug application

Micromechanical methods, such as silicon micro-cantilever arrays and vertical PDMS micro-column arrays, also have disadvantages such as complex chip processing technology, complex experimental operations, and high costs.

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