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Preparation method of sea cucumber polypeptide

A technology of sea cucumber polypeptides and sea cucumbers, which is applied in the field of preparation of sea cucumber polypeptides, which can solve the problems of excessive heavy metal content, large molecular weight, and difficulty in absorption, and achieve the effects of low wastewater discharge, mild reaction conditions, and reduced energy consumption

Inactive Publication Date: 2015-01-07
冯群力
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, sea cucumber polypeptides are mostly composed of hundreds of amino acids connected by peptide bonds, and their molecular weight is relatively large. In sea cucumber products prepared by traditional methods, the sea cucumber polypeptides are surrounded by the sea cucumber body wall of macromolecular chains. If traditional eating methods are used, Difficult to be fully absorbed by the body, the absorption rate is only 5-18%
In addition, due to the influence of growth environment factors, sea cucumbers are often enriched in heavy and harmful metal elements such as arsenic, mercury, and lead. It is not uncommon for pollution and other problems to cause excessive heavy metal content in sea cucumbers. Traditional eating methods will force the human body to absorb them, and long-term consumption will have adverse effects on human health.

Method used

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  • Preparation method of sea cucumber polypeptide
  • Preparation method of sea cucumber polypeptide
  • Preparation method of sea cucumber polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Select fresh Liao sea cucumber as sea cucumber raw material to prepare sea cucumber polypeptide:

[0020] 1) Screening of raw materials: Screening and preparing some fresh sea cucumbers;

[0021] 2) Cleaning of raw materials: Wash the screened japonicus japonicus with tap water first, and then clean it with ultrapure water with a conductivity of 10 Us / cm, so as to wash away the silt in the japonicus japonicus japonicus;

[0022] 3) Low-temperature enzymatic hydrolysis: Put ultrapure water with a conductivity of 5 Us / cm equal to the weight of sea cucumber in the biological enzymatic hydrolysis tank, and heat it to 60°C; then put it in the biological enzymatic hydrolysis tank to wash Apostichopus japonicus, heated to 50°C; put in 0.5% papain by weight of Apostichopus japonicus, and enzymolyze it at 50°C for 3 hours; Enzymolysis treatment for 15 hours; put in activated carbon with 0.5% weight of sea cucumber, and place for 1 hour; put in quartz sand with 1% wei...

Embodiment 2

[0027] Example 2: Select fresh sea cucumber as sea cucumber raw material to prepare sea cucumber polypeptide:

[0028] 1) Raw material screening: screening and preparing some fresh sea cucumbers;

[0029] 2) Cleaning of raw materials: Wash the screened sea cucumber with tap water first, and then clean it with ultra-pure water with a conductivity of 15 Us / cm, so as to wash away the sediment in the sea cucumber body;

[0030] 3) Low-temperature enzymatic hydrolysis: put ultrapure water with a conductivity of 2 Us / cm equal to that of sea cucumber in the biological enzymatic hydrolysis tank, and heat it to 55°C; then put it in the biological enzymatic hydrolysis tank and wash of sea cucumber, heated to 48°C; put in 0.6% papain by weight of sea cucumber, and enzymatically treat it at 48°C for 2.5h; put in collagenase of 0.6% by weight of sea cucumber, Enzyme hydrolysis treatment for 18 hours; put 0.5% of the weight of sea cucumber in activated carbon, and let it stand for 1.5 hour...

Embodiment 3

[0035] Example 3: Select fresh plum blossom as the sea cucumber raw material to prepare sea cucumber polypeptides:

[0036] 1) Raw material screening: screening and preparing some fresh plum blossom ginseng;

[0037] 2) Cleaning of raw materials: Wash the screened plum blossom ginseng with tap water first, and then wash it with ultrapure water with a conductivity of 5 Us / cm to clean the sediment in the plum blossom ginseng body;

[0038]3) Low-temperature enzymatic hydrolysis: put ultrapure water with a conductivity of 0.1 Us / cm equal to the weight of plum flower ginseng in the biological enzymatic hydrolysis tank, and heat it to 60°C; then put it into the biological enzymatic hydrolysis tank to wash Plum blossom ginseng, heated to 52°C; put in 0.4% by weight of plum blossom ginseng with papain, and enzymolyze at 52°C for 4 hours; put in 0.5% by weight of plum blossom ginseng, and enzymolyze at 52°C 18h; put in activated carbon with 0.5% weight of plum blossom ginseng and let...

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Abstract

The invention discloses a preparation method of a sea cucumber polypeptide. The method comprises the following steps: screening raw materials; cleaning the raw materials; carrying out low-temperature enzymolysis; carrying out first concentration filtration; carrying out second concentration filtration; and drying. The reaction condition in the whole preparation process disclosed by the invention is mild; and the activity of the sea cucumber polypeptide can be ensured. Low-temperature enzymolysis at 40-60 DEG C is adopted, and enzyme cutting sites of sea cucumber protein is subjected to alternative splicing through papain and collagenase, so that the enzymolysis of the sea cucumber protein is relatively thorough; and the sea cucumber protein with high molecular weight is decomposed into a plurality of micro-molecule polypeptides with smaller molecular weight, so that the absorption requirements of a human body are met, full absorption of the human body on the sea cucumber polypeptide is facilitated, and the absorptivity can reach over 80%. The sea cucumber polypeptide prepared by the method disclosed by the invention is very low in harmful heavy metal ion content, and safe and non-toxic. The preparation method disclosed by the invention is low in wastewater discharge in the preparation process and beneficial to reduction of energy consumption, is capable of utilizing the value of the sea cucumber to the maximal extent, and has important social and economic significance.

Description

technical field [0001] The invention relates to a preparation method of a polypeptide, in particular to a preparation method of a sea cucumber polypeptide. Background technique [0002] Sea cucumbers began to exist as early as the Cambrian period about 600 million years ago. They are one of the earliest living species and are vividly called "marine living fossils". Sea cucumber is a rare high-protein, low-fat, low-sugar, and cholesterol-free natural nutritious food in the world. Its nutritional content is not only rich, but also balanced and reasonable. People compare it to the nutritional treasure house in the sea. Sea cucumbers contain many substances with important biological activities, including sea cucumber toxins, sea cucumber polysaccharides, sea cucumber saponins, sea cucumber polypeptides, fatty acids, sea cucumber gangliosides, etc. Among them, sea cucumber polysaccharides and sea cucumber saponins have anti-cancer, anti-tumor, and enhance Immunity, anti-coagula...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 冯群力
Owner 冯群力
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