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Triiodothyroxin hapten luminous marker and synthetic method thereof

A technology of luminescent markers and thyroxine, which is applied in the direction of organic chemistry, can solve the problems of high price and easy to produce differences, and achieve the effect of easy purification and improved detection sensitivity

Inactive Publication Date: 2015-01-07
SHENZHEN MAXCHEMTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the shortcomings of protein macromolecular compounds used in previous detection reagents, which are expensive and prone to differences in the production process

Method used

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  • Triiodothyroxin hapten luminous marker and synthetic method thereof
  • Triiodothyroxin hapten luminous marker and synthetic method thereof
  • Triiodothyroxin hapten luminous marker and synthetic method thereof

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Embodiment Construction

[0019] The present invention will be described in further detail below in conjunction with specific embodiments.

[0020] The synthetic route of the novel compound used for the detection of triiodothyronine by chemiluminescence method according to the present invention is as follows:

[0021]

[0022] specific:

[0023] Preparation of compound 2

[0024] 11.55 g of bisnonyltriamine was dissolved in 100 ml of acetonitrile, and 1.56 g of ethyl trifluoroacetate was added.

[0025] 1. Stir overnight at room temperature.

[0026] 2. Monitor the completion of the reaction by thin layer chromatography.

[0027] 3. Concentrate under reduced pressure and pump to dryness to obtain 2 g of crude product of trifluoroacetamide compound 2 (Formula: C20H39F6N3O4S2, MW: 563.66).

[0028] Preparation of compound 3

[0029] 1. Dissolve the crude product of trifluoroacetamide (compound 2) in 100ml of acetonitrile, and first add 0.875ml of triethylamine.

[0030] 2.0.74g of succinic anhyd...

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Abstract

The invention discloses a triiodothyroxin hapten luminous marker and a synthetic method thereof. The synthetic method of the triiodothyroxin hapten luminous marker comprises the following steps: A) preparing a trifluoroacetamide crude product; B) preparing trifluoroacetamide; C) further preparing trifluoroacetamide; D) preparing triiodothyroxin methyl ester; E) further preparing triiodothyroxin methyl ester; F) further preparing triiodothyroxin methyl ester; and G) preparing a target product, namely acridine-marked triiodothyroxin methyl ester. The invention discloses a novel compound for detecting triiodothyroxin hapten by virtue of a chemical luminescence method as well as a synthetic route and a synthetic method of the compound. The marker of the small molecules is easy to purify, can be industrially produced and improves the detection sensitivity. The defects that the protein macromolecular compounds used by the conventional detection reagent have high price, difference easily produced in a production process, and the like are overcome.

Description

technical field [0001] The invention belongs to the field of organic synthesis, in particular to a synthesis method of a triiodothyronine hapten luminescent marker. Background technique [0002] Chemiluminescence immunoassay is a non-radioactive immunoassay technology that develops very rapidly in the world. It is an ultra-high sensitivity trace detection technology developed after enzyme immunoassay, radioimmunoassay and fluorescence immunoassay. It has the advantages of high sensitivity, wide linear range, simple and fast operation, good stability of markers, no pollution, stable results, etc. It is currently the most ideal method for immunoquantitative analysis. In chemiluminescent immunoassays, commonly used luminescent substances include luminol, isoluminol, acridinium esters, and 1,2-dioxetane compound (1,2-dioxetane compound). Among them, isoluminol and acridinium ester are directly marked as tracer molecules, which belong to flash chemiluminescent reaction. Luminol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D219/04
CPCC07D219/04
Inventor 任琪谈宇清宫磊
Owner SHENZHEN MAXCHEMTECH
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