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Method for promoting germination of alfalfa seeds

A technology for alfalfa and seeds, applied in the directions of seed and rhizome treatment, application, agriculture, etc., can solve the problems affecting the germination rate and emergence rate of seeds, uneven crop emergence, and hard seeds and impermeable water, etc., to improve germination potential and germination rate, non-pathogenicity and strong stress resistance, the effect of increasing yield

Inactive Publication Date: 2015-01-07
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Because alfalfa is a leguminous crop, its seeds have a high hard seed rate, and when they germinate, the seeds are hard and impervious to water or absorb water late, thereby affecting the germination rate and emergence rate of the seeds. After sowing, no seedlings or late seedlings emerge, resulting in crop emergence Irregularity, the phenomenon of lack of seedlings and broken ridges, also brings great difficulties to the eradication of weeds

Method used

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  • Method for promoting germination of alfalfa seeds
  • Method for promoting germination of alfalfa seeds
  • Method for promoting germination of alfalfa seeds

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Disinfection treatment of alfalfa seeds: select a batch of alfalfa seeds, first treat them with 70% ethanol for 1 minute, then use 2% NaClO to disinfect them for 10 minutes, and then rinse them with sterile water for 7 times, each time for 3 minutes. Agitate to rinse the alcohol and sodium hypochlorite off the surface of the seeds. Then the fully sterilized alfalfa seeds were soaked in sterile water for 24 hours in the dark.

[0024] Preparation of Bacillus subtilis GB03 bacterial liquid: first prepare the lysis broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, and the high temperature to 132 ℃ sterilization, after the cooling temperature is 20 ℃, add 100 μL of Bacillus subtilis GB03 bacterial liquid per 100 mL of LB liquid medium, and cultivate overnight on a constant temperature culture shaker at 30 ℃ at a speed of 250 r / min. Determination of bacterial c...

Embodiment 2

[0028] Disinfection treatment of alfalfa seeds: select a batch of alfalfa seeds, first treat them with 70% ethanol for 1 minute, then use 2% NaClO to disinfect them for 10 minutes, and then rinse them with sterile water for 8 times, each time for 3 minutes. Agitate to rinse the alcohol and sodium hypochlorite off the surface of the seeds. Then the fully sterilized alfalfa seeds were soaked in sterile water for 24 hours in the dark.

[0029] Preparation of Bacillus subtilis GB03 bacterial liquid: first prepare the lysis broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, and the high temperature to 115 Sterilize at ℃, after the cooling temperature is 20℃, add 50μL of Bacillus subtilis GB03 bacterial solution to every 100mL of LB liquid medium, cultivate overnight on a constant temperature culture shaker at 30℃ at a speed of 180r / min, Determination of bacterial conce...

Embodiment 3

[0033] Disinfection treatment of alfalfa seeds: select a batch of alfalfa seeds, first treat them with 68% ethanol for 1.2 minutes, then use 2.2% NaClO to disinfect them for 9 minutes, and then rinse them with sterile water 8 times, each time for 3.2 minutes. Stir constantly in the middle, rinse the alcohol and sodium hypochlorite on the surface of the seeds. Then the fully sterilized alfalfa seeds were soaked in sterile water for 23.5 hours in the dark.

[0034] Preparation of Bacillus subtilis GB03 bacterial liquid: first prepare the lysis broth LB liquid medium: prepare according to the ratio of sodium chloride, tryptone and yeast powder at 1:2:1, the solvent is distilled water; adjust the pH to 7.2, and the high temperature to 115 Sterilize at ℃, after the cooling temperature is 20℃, add 50μL of Bacillus subtilis GB03 bacterial solution to every 100mL of LB liquid medium, cultivate overnight on a constant temperature culture shaker at 30℃ at a speed of 180r / min, Determina...

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Abstract

The invention relates to a method for promoting germination of alfalfa seeds. The method comprises the steps of soaking the alfalfa seeds in bacillus subtilis GB03 liquid, then taking out the alfalfa seeds and carrying out germination. According to the method for treating the alfalfa seeds, the germination potential and the germination rate of the alfalfa seeds can be effectively and obviously improved, the phenomenon that alfalfas do not germinate or germinate late and do not germinate uniformly after being sowed and seedlings cannot fill in whole ridges are relieved, and the yield of forage per unit area is increased; meanwhile, bacillus subtilis has the characteristics of no toxicity or harm to human and livestock, no pollution to the environment, high non-pathogenicity, high stress resistance and the like. The method for promoting the germination of alfalfa seeds is easy to operate, can be widely applied to sowing of forage and has an important economical value.

Description

technical field [0001] The invention relates to the field of forest and grass planting, in particular to a method for promoting germination of alfalfa seeds. Background technique [0002] Alfalfa is a perennial herb with strong roots and a well-developed root neck. It is an excellent leguminous forage and has the reputation of "King of Forage". With the rapid development of grassland agriculture in my country, in recent years, alfalfa has been developed and planted on a large scale as the main pasture, which makes the output of alfalfa increase day by day. Because alfalfa is a leguminous crop, its seeds have a high hard seed rate, and when they germinate, the seeds are hard and impervious to water or absorb water late, thereby affecting the germination rate and emergence rate of the seeds. After sowing, no seedlings or late seedlings emerge, resulting in crop emergence Irregularity, the phenomenon of lack of seedlings and broken ridges appeared, which also brought great dif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00
Inventor 吕昕培韩庆庆张金林李静葛静李惠茹王锁民
Owner LANZHOU UNIVERSITY
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