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System for simultaneous representation of single cell Young's modulus and cell membrane specific capacitance

A Young's modulus, single-cell technology, applied in the direction of measuring electrical variables, measuring resistance/reactance/impedance, measuring devices, etc., can solve the problems of not being able to characterize the inherent mechanical/electrical characteristic parameters of cells, and achieve the effect of low cost

Active Publication Date: 2014-12-31
INST OF ELECTRONICS CHINESE ACAD OF SCI
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Problems solved by technology

[0005] In the process of realizing the present invention, the applicant found that the existing method for high-throughput simultaneous characterization of cell mechanical / electrical properties has the following defects: that is, it can only characterize some biophysical property parameters that depend on cell size, such as the time to enter the compression channel , cantilever beam deformation, impedance spectrum, etc., cannot characterize the inherent mechanical / electrical characteristic parameters of the cell, that is, the Young's modulus of the cell and the specific capacitance of the cell membrane

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  • System for simultaneous representation of single cell Young's modulus and cell membrane specific capacitance
  • System for simultaneous representation of single cell Young's modulus and cell membrane specific capacitance
  • System for simultaneous representation of single cell Young's modulus and cell membrane specific capacitance

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Embodiment Construction

[0027] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be described in further detail below in conjunction with specific embodiments and with reference to the accompanying drawings. It should be noted that, in the drawings or descriptions of the specification, similar or identical parts all use the same figure numbers. Implementations not shown or described in the accompanying drawings are forms known to those of ordinary skill in the art. Additionally, while illustrations of parameters including particular values ​​may be provided herein, it should be understood that the parameters need not be exactly equal to the corresponding values, but rather may approximate the corresponding values ​​within acceptable error margins or design constraints. The directional terms mentioned in the embodiments, such as "upper", "lower", "front", "rear", "left", "right", etc., are only referring to the directions of the...

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Abstract

The invention provides a system for simultaneous representation of single cell Young's modulus and cell membrane specific capacitance based on a microfluidic technology. The system makes a cell equivalent to an isotropic super-viscoelastic body, based on ABAQUS mechanical simulation, the relation between the displacement generated by instantaneous entrance of the cell front end into a compression channel, and the cell size, the Young's modulus, the pressure intensity, and geometric parameters of the compression channel, and the Young's modulus of the cell can be calculated. In addition, the invention also puts forward an equivalent electrical model for the cell to pass through the compression channel, the relation between the cell membrane capacitance, cytoplasm resistance, cell and compression channel wall leakage resistance, electrical parameters of the compression channel itself, and the impedance spectrum can be obtained, the impedance change caused by passing of the cell through the compression channel is converted to cell membrane specific capacitance, so that the cell membrane specific capacitance of the single cell can be calculated, thus realizing synchronous measurement of the single cell Young's modulus and cell membrane specific capacitance.

Description

technical field [0001] The invention relates to the field of biological information detection, in particular to a system for simultaneously characterizing single-cell Young's modulus and cell membrane specific capacitance. Background technique [0002] As the basic unit of life activities, cells contain various biomolecules that interact with each other to form a busy and orderly system. The mechanical characteristic parameter of the cell is Young's modulus, which is determined by the cytoskeleton; the electrical characteristic parameter of the cell is the cell membrane specific capacitance (capacitance per unit area of ​​the cell membrane), which is composed of phospholipid bilayer and membrane protein. As important functional units of cells, cytoskeleton and cell membrane participate in important physiological functions such as cell proliferation and division, and are closely related to cell state. Although traditional characterization techniques such as atomic force micr...

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Application Information

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IPC IPC(8): G01N15/10G01R27/26
Inventor 陈健赵阳罗亚娜龙荣陈德勇王军波
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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