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Penicillium expansum FP2 strain and application thereof

A technology that expands Penicillium and FP2 and is applied in the field of microorganisms, which can solve the problems of restricting research work, high price, increasing the cost of scientific research work, etc., and achieve the effect of reducing costs

Inactive Publication Date: 2014-12-31
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the commercially available patulin standard product is expensive, as: the price of patulin P1639-5MG (5mg) with more than 98% purity of sigma company is 1946.88 yuan, and the price of P1639-10MG (10mg) is 3601.26 yuan RMB
This greatly increases the cost of scientific research work and limits research work to a certain extent

Method used

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  • Penicillium expansum FP2 strain and application thereof
  • Penicillium expansum FP2 strain and application thereof
  • Penicillium expansum FP2 strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The strain named Penicillium extensa FP2 is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2014040.

[0047] The ITS-5.8S rDNA sequence of Penicillium elongatus FP2 strain is:

[0048]

[0049] The screening method of the above-mentioned Penicillium extensa FP2 strain is as follows:

[0050] 1. Separation and purification

[0051] Sterile inoculation needles were dipped to pick up the mildew spots on 3 different rotten apples, streaked and inoculated on 6 PDA solid medium plates, and cultured at 28°C. After the grown colonies have obvious spores, dip the spores with a sterile inoculation needle and streak them on the PDA solid medium plate respectively, and culture them at 28°C for 5 days. Pick the edge hyphae of a single colony and culture them on 12 PDA slopes at 28°C for 7 days, add 10 mL of sterile water, dip the spore suspension with a sterile inoculation needle, streak on 12 PDA solid medium plates, and purify...

Embodiment 2

[0067] The Penicillium extensa FP2 bacterial strain that adopts embodiment 1 screening is in the purposes of preparing patulin, and its method of use is as follows:

[0068] 1. Fermentation

[0069] Take 28 250mL triangular flasks, fill each bottle with 100g peeled apple pieces as the culture medium, add 8 layers of gauze plugs, and sterilize at 121°C for 20 minutes. Inoculate the slant of the PDA test tube with Penicillium extensa FP2 strain, activate it at 28°C for 7 days, add sterile water to the slant of each test tube to prepare a concentration of 10 7 cfu / mL spore suspension, insert 1mL concentration of 10 into each Erlenmeyer flask filled with apple culture medium 7 The cfu / mL spore suspension was cultured at 28° C. in the dark for 10 days to obtain a culture.

[0070] 2. Separation

[0071] Take 28 bottles of culture, grind them into paste respectively, add 100mL of ethyl acetate to each bottle, shake and extract at 150-200r / min in the dark for 1 hour, let stand for...

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Abstract

The invention discloses a penicillium expansum strain named as Penicillium expansum FP2, the scientific name is Penicillium expansum FP2, and the preservation number is CCTCC NO:M2014040. The screened penicillium expansum FP2 strain is used for preparation of patulin, during the fermentation step of preparation of the patulin, due to use of an apple culture medium, the content of the patulin in a prepared crude extract is 300 times of the content of the patulin prepared by a potato dextrose agar (PDA) liquid culture medium, and the cost of the product is 1 / 380 of the cost of the product of the same kind of sigma company, and the cost of the product is greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacterial strain of Penicillium expanses. Background technique [0002] Molds can produce a variety of secondary metabolites with various biological activities. Some molds also produce toxic secondary metabolites—mycotoxins when they grow on substrates. These toxins can cause various diseases in humans and animals. Patulin (PAT), also known as patulin, is a highly toxic fungal metabolite with colorless prismatic crystals and a molecular formula of C 7 h 6 o 4 , the molecular weight is 154, and the chemical name is 4-hydroxy 4-hydro-furan (3,2-carbon) pyran-2 (6-hydrogen) ketone [4-hydroxy-4-H-furo (3,2c) pyran -2(6H)-one]. It is found in many fruits spoiled by mold, such as apples, apricots, blueberries, cherries, grapes, pears, peaches and plums and their products. Apples are most likely to accumulate this toxin when moldy. [0003] A large number of st...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P17/18C12R1/83
Inventor 张晓瑞郭玉蓉马瑜柴永海
Owner SHAANXI NORMAL UNIV
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