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Method for rapidly detecting toxicity of beans

A detection method and toxicity technology are applied in the field of rapid detection of kidney bean toxicity, which can solve the problems of lack of a systematic method for the comprehensive toxicity of kidney bean, and achieve the effects of high accuracy, simple operation and strong comprehensiveness of the method.

Active Publication Date: 2014-12-24
JIANGHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many related reports on toxicity, and there are very few studies on the toxicity of certain anti-nutritional factors in kidney beans, but there is still a lack of systematic methods for the detection of comprehensive toxicity in kidney beans

Method used

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  • Method for rapidly detecting toxicity of beans
  • Method for rapidly detecting toxicity of beans

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Taking the common bean variety Bean Saint esprit as raw material, obtaining the common bean extract according to the previous method, and measuring the lectin content of the common bean variety X 1 is 10.85, and then substitute into the formula:

[0027] Y 2 =30.837+4.5X 1

[0028] get Y 2 The value is 79.66, according to the above Y 2 The meaning of the value shows that the species belongs to the species with high toxicity. The Y of the new bean germplasm was determined by controlling the measurement of lectins under the same conditions. 2 value, the toxicity level can be judged.

Embodiment 2

[0030] Taking the common bean variety heat-resistant frame bean as a raw material, obtaining the common bean extract according to the previous method, and measuring the lectin content of the common bean variety X 1 is 4.02, and then substitute into the formula:

[0031] Y 2 =30.837+4.5X 1

[0032] get Y 2 The value is 48.93, according to the above Y 2 The meaning of the value shows that the species belongs to the species with moderate toxicity.

Embodiment 3

[0034] Taking the common bean variety nine grain white as the raw material, obtaining the common bean extract according to the previous method, and measuring the lectin content of the common bean of the variety X 1 is 0.85, and then substitute into the formula:

[0035] Y 2 =30.837+4.5X 1

[0036] get Y 2 The value is 34.66, according to the above Y 2 The meaning of the value shows that the species belongs to the species with low toxicity.

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Abstract

The invention discloses a method for rapidly detecting toxicity of beans. The method comprises the following steps of processing the beans into extract liquid, measuring the content of lectin in the extract liquid, calculating the toxicity level of the beans by utilizing a multi-element regression equation Y2=30.837+4.5X1, wherein X1 is the content of the plant lectin, and Y2 represents the growth influencing rate of mouse cells. The Y2 value is solved by measuring the lectin of new beans under the same condition, and the toxicity level of the beans can be judged. The method is high in systematicness and comprehensiveness; the method is simple to operate, wide in applicability and capable of helping the evaluation of toxicity of other plants; the toxicity of unknown beans can be judged; the accuracy for predicting the toxicity level of the beans is high.

Description

technical field [0001] The invention relates to the evaluation of the toxicity of vegetable crops, in particular to a rapid detection method for the toxicity of kidney beans. Background technique [0002] Phaseolus vulgaris L. is an annual herb belonging to the family Leguminosa and Papilionideae. It is the most grown edible legume in the world, accounting for 50% of the global edible legume production (excluding soybeans). Divided into podded bean and common bean for grain use, podded bean is rich in nutrients, low in fat and high in protein, and rich in minerals and vitamins. It is an important crop in the world. [0003] Improper processing of kidney beans can easily lead to numbness of human limbs, burning stomach pain, headache, chest tightness and other neurological symptoms. In severe cases, coma can even cause damage to multiple organs of the heart, kidneys and liver or lead to sudden death. my country is a big country in the cultivation and consumption of common b...

Claims

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Application Information

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IPC IPC(8): G01N33/02G01N21/31
Inventor 陈禅友尚蕊李佳楠吴华胡志辉郭瑞潘磊
Owner JIANGHAN UNIVERSITY
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