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cKGM (cation-modified konjac glucomannan) and nucleic acid medicine nanometer preparation, preparation method and application

A technology of konjac polysaccharides and nucleic acid drugs, which is applied in the field of biomedicine, can solve the problems of high preparation conditions, complicated preparation process, and high production cost, and achieve the effect of simple process, easy acquisition, and high cost

Inactive Publication Date: 2014-12-24
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional nano-preparation has the disadvantages of complicated preparation process, high requirements for preparation conditions, and relatively expensive preparation equipment, which leads to high production costs.
In addition, traditional nano-preparations still have disadvantages such as low encapsulation efficiency and bioavailability.

Method used

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  • cKGM (cation-modified konjac glucomannan) and nucleic acid medicine nanometer preparation, preparation method and application
  • cKGM (cation-modified konjac glucomannan) and nucleic acid medicine nanometer preparation, preparation method and application
  • cKGM (cation-modified konjac glucomannan) and nucleic acid medicine nanometer preparation, preparation method and application

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Embodiment Construction

[0012] During the implementation of the present invention, the present invention mainly investigates the synthesis of cKGM, the preparation of the complex of nucleic acid and cKGM, and the ability of nanoparticles compounded with nucleic acid and cKGM to enter macrophages. The specific implementation process is as follows.

[0013] 1. Preparation of cKGM

[0014] The synthetic method of cKGM: take the konjac polysaccharide of 500mg (purchased from Megazyme Company, Ireland) and dissolve in 25ml of methacrine (purchased from Sigma Company, the U.S.), and prepare the dimethylforme solution of the konjac polysaccharide of 20mg / ml , then add 1.5g of N,N'-carbonyldiimidazole (CDI, available from Sigma, U.S.) and stir at room temperature for 1 hour, then add 2.5ml of anhydrous ethylenediamine (available from Sigma, U.S.), and stir the reaction After 12 hours, the reaction solution was transferred into a dialysis bag, and dialyzed in double distilled water for 3 days, changing the wa...

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a cKGM (cation-modified konjac glucomannan) and nucleic acid medicine nanometer preparation, a preparation method and an application. The preparation consists of nucleic acid and cKGM. The specific preparation method comprises steps as follows: the ethylenediamine-modified KGM is prepared into the cKGM, and then the cKGM is compounded with a nucleic acid medicine to form nanoparticles. The nucleic acid in the preparation is ribonucleic acid or deoxyribonucleic acid, the preparation has the characteristic of targeting macrophages and can be prepared into medicines for treating inflammations caused by the macrophages.

Description

1. Technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a cationic konjac polysaccharide nucleic acid drug nano-preparation, a preparation method and an application. 2. Background technology [0002] Macrophages are tissue-located white blood cells derived from monocytes in the peripheral blood. Both macrophages and monocytes are phagocytic cells that participate in innate and acquired immunity in vertebrates. Their main function is to phagocytize (i.e. phagocytize and digest) cell debris and pathogens in the form of fixed or free cells, and activate lymphocytes or other immune cells to respond to pathogens. However, under pathological conditions, the abnormal activation of macrophages will produce a large number of inflammatory factors, chemokines and free radicals, which will further intensify the inflammatory response and cause damage to tissues and organs. Nucleic acid drugs targeting key transcription factors...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K47/36A61P29/00
Inventor 董磊张峻峰黄振
Owner NANJING UNIV
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