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Method for determination of blood-nourishing and brain-clearing effective component

An active ingredient and a technology for nourishing serum and brain, applied in the field of determination of active ingredients for nourishing serum and brain, and can solve problems such as the complex material basis of traditional Chinese medicine

Active Publication Date: 2014-12-17
天士力东北现代中药资源有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex material basis of traditional Chinese medicine, no one has conducted pharmacokinetic experiments on the main chemical components in the brain of nourishing serum before. Therefore, it is necessary to develop a content determination method with good specificity, high sensitivity, and quantitative accuracy to determine the content of effective substances in plasma.

Method used

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  • Method for determination of blood-nourishing and brain-clearing effective component
  • Method for determination of blood-nourishing and brain-clearing effective component
  • Method for determination of blood-nourishing and brain-clearing effective component

Examples

Experimental program
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Effect test

Embodiment 1

[0122] Assay method of the present invention, the steps are as follows:

[0123] (1) Sample treatment: take 1 volume of plasma sample, add 1 volume of 1mol / L hydrochloric acid solution and 1 volume of internal standard chloramphenicol 200ng / ml solution, vortex and mix for 30s; then add 30 times the volume of ethyl acetate and vortex mix for 3min Carry out liquid-liquid extraction; the above mixed solution was centrifuged at 4500r / min, and the ethyl acetate layer was taken, dried under nitrogen in a water bath at 35°C, and then used, and then redissolved with 1 volume of mobile phase 80%A:20%B, vortexed Spin for 30s, centrifuge at 12000r / min for 3min, then take the supernatant and inject samples for determination;

[0124] (2) Determination by liquid mass spectrometry:

[0125] Liquid phase separation: The liquid phase part of the liquid chromatography-mass spectrometer uses a reversed-phase c18 silica gel chromatographic column as the separation medium, and gradient elution a...

Embodiment 2

[0138] A method for determining the active ingredients of nourishing serum and brain, the steps are as follows:

[0139] (1) Sample treatment: Take 1 volume of plasma sample, add 0.2 volume of hydrochloric acid solution and 0.2 volume of ketoprofen internal standard solution, and vortex mix; then add 10 volumes of ethyl acetate organic solvent, vortex mix for liquid-liquid extraction; the above mixing After the solution was centrifuged, the organic solvent layer was taken, dried, and then reconstituted with 1 volume of liquid phase (mobile phase 60% A, the rest was B) and then centrifuged, and the supernatant was sampled for determination;

[0140] (2) Determination by liquid mass spectrometry:

[0141] Among them, the chromatographic conditions of the liquid phase: use reversed-phase c18 or c8 silica gel chromatographic column as the separation medium, gradient elution after sample injection, among them, mobile phase A0.02% formic acid, acetic acid or water, B acetonitrile or...

Embodiment 3

[0155] A method for determining the active ingredients of nourishing serum and brain, the steps are as follows:

[0156] (1) Sample treatment: take 3 volumes of plasma samples, add 2 volumes of formic acid solution and 1 volume of Danshensu internal standard solution, and vortex mix; then add 50 volumes of organic solvents and vortex mix for liquid-liquid extraction; the above mixed solution is centrifuged and taken The organic solvent layer was blown dry, then reconstituted with 2 volumes of liquid phase (mobile phase 80% A, the rest was B) and centrifuged, and the supernatant was sampled for determination;

[0157] (2) Determination by liquid mass spectrometry:

[0158] Among them, the chromatographic conditions of the liquid phase: use reversed-phase c18 or c8 silica gel chromatographic column as the separation medium, gradient elution after sample injection, among them, mobile phase A5% formic acid, acetic acid or water, B acetonitrile or methanol; gradient elution conditi...

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Abstract

The invention relates to a drug detection method, and in particular relates to a method for determination of a blood-nourishing and brain-clearing effective component. The method comprises the following steps: (1) sample treatment: taking a plasma sample, adding an acid solution and an internal standard solution, and carrying out vortex mixing; followed by adding an organic solvent, carrying out vortex mixing, and carrying out liquid-liquid extraction; and after the mixed liquid is centrifuged, taking the organic solvent layer, blow-drying, and then re-dissolving with a mobile phase, centrifuging, taking the supernatant, and injecting the sample for determination; and (2) determining by a liquid chromatograph-mass spectrometer.

Description

technical field [0001] The invention relates to a drug detection method, in particular to a method for measuring active ingredients of nourishing serum and brain. Background technique [0002] Yangxue Qingnao Granule is a modern Chinese medicine exclusively developed and produced by Tasly Group based on the traditional theory of traditional Chinese medicine and using the latest technology. In 1999, it was awarded the national new drug invention patent. , forgetfulness, etc. have significant curative effects, and have become the key products of Tasly Group. The formula of Yangxue Qingnao Granules is described in "A Traditional Chinese Medicine for Treating Headaches", the patent number is 931000505. Its composition includes 4-9% of Angelica sinensis, 4-9% of Chuanqiong, 2-8% of Radix Paeoniae Alba, Rehmannia Rehmanniae 2-8%, Incarnation 10-15%, Mildew Spatholobus 10-15%, Prunella vulgaris 10-15%, Cassia Seed 10-15%, Mother of Pearl 10-15%, Yuanhu 4-9%, Asarum 0.5-2%. "Yang...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 王相阳马晓慧李伟褚扬郭嘉华李淑明周水平朱永宏
Owner 天士力东北现代中药资源有限公司
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