Quick tolclofos-methyl colloidal gold detection test strip and preparation method thereof

A technology of tolclofos-methyl and tolclofos-methyl, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of ELISA method application limitation, difficult to meet fast, online detection, and detection results are not intuitive enough, etc. Achieve the effect of showing the image of the results, facilitating promotion, and judging the image

Inactive Publication Date: 2014-12-10
JURONG AGRI TECH PROMOTION CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Instrumental analysis methods include high performance liquid chromatography (High performance liquid chromatography, HPLC), gas chromatography (Gas chromatography, GC), thin layer chromatography (Thin layer chromatography, TLC), liquid-mass tandem method (HPLC-MS) Although these instrumental analysis methods can achieve high detection sensitivity, they require complex and expensive instruments and professional operators. In addition, the sample pretreatment is cumbersome and time-consuming, and the detection cost is high. The need for fast, online testing
ELISA (Enzyme Linked Immunosorbent Assay) method is a commonly used immunoassay method, but it has relatively many operating steps, a long detection time, the detection result is not intuitive enough, and it cannot be detected online.
Therefore, the application of ELISA method is greatly limited.

Method used

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  • Quick tolclofos-methyl colloidal gold detection test strip and preparation method thereof
  • Quick tolclofos-methyl colloidal gold detection test strip and preparation method thereof
  • Quick tolclofos-methyl colloidal gold detection test strip and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A colloidal gold rapid detection test strip for tolclofos-methyl, the test strip contains a liner 1 and a sample pad 2, a gold standard binding pad 3, a cellulose film 4 and a water-absorbent pad 7 arranged in sequence on the liner , the liner 1 is a tough material that does not absorb water, and the tough material is made of hard plastic strips, the gold-labeled binding pad 3 is adsorbed with a gold-labeled antibody for tolclofos-methyl, and the cellulose film There are invisible detection line 5 and invisible control line 6 on the 4, the invisible detection line 5 is printed with the carrier protein solution coupled with methyl tolclofos hapten, and the invisible control line 6 is printed with rabbit anti-mouse IgG antibody . The water-absorbing pad 7 is water-absorbing filter paper or oil filter paper with strong water-absorbing capacity. Tolclofos-methyl gold-labeled antibody is colloidal gold-labeled tolclofos-methyl monoclonal antibody. The carrier protein for c...

Embodiment 2

[0037] It is basically the same as Example 1, except that the tough material is made of non-absorbent hard paper strips, and the water-absorbing pad 7 is oil filter paper with strong water-absorbing capacity. The carrier protein for coupling tolclofos-methyl hapten is chicken ovalbumin OVA. The sample pad 2 is made of nylon film, polyvinylidene fluoride film and polyester film. Cellulose membranes are pure cellulose membranes.

Embodiment 3

[0039]It is basically the same as in Example 1, except that the tough material is made of hard plastic strips, and the water-absorbing pad 7 is water-absorbing filter paper with strong water-absorbing capacity. The carrier protein for coupling tolclofos-methyl hapten is human serum albumin HAS. The sample pad 2 is made of polyvinylidene fluoride film and polyester film. The cellulose film is a carboxylated cellulose film.

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Abstract

The invention discloses a quick tolclofos-methyl colloidal gold detection test strip. The quick tolclofos-methyl colloidal gold detection test strip comprises a substrate and a sample pad, a gold-labeled combination pad, a cellulose film and a water absorption pad which are arrayed on the substrate in sequence, wherein a tolclofos-methyl gold-labeled antibody is adsorbed in the gold-labeled combination pad; a hidden detection line and a hidden contrast line are arranged on the cellulose film; the hidden detection line is printed by a carrier protein solution coupled with a tolclofos-methyl hapten; the hidden contrast line is printed by a rabbit antimouse IgG antibody. The quick tolclofos-methyl colloidal gold detection test strip has the advantages of high specificity, high sensitivity, good simplicity and high speed; the result is vivid, intuitive and accurate.

Description

technical field [0001] The invention belongs to the technical field of immune analysis of pesticide residues, and in particular relates to a rapid detection test strip of tolclofos-methyl colloidal gold and a preparation method thereof. Background technique [0002] Tolclofos-methyl is a broad-spectrum organophosphorus insecticide used to control pests in stored grains and various foliage crops, and is also used to control adult mosquitoes, flies, Aquatic larvae and sanitary pests. Its mechanism of action is to inhibit the activity of acetylcholinesterase. Acetylcholine is a neurotransmitter, and excess acetylcholine overstimulates acetylcholine receptors, leading to the death of target organisms. However, some creatures that were not the intended target were also poisoned, including humans, fish and other creatures. [0003] At present, the detection methods for pesticide residues are mainly instrumental analysis methods and enzyme-linked immunoassay methods (ELISA). In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/544G01N33/532
CPCG01N33/544G01N33/532
Inventor 韩志成
Owner JURONG AGRI TECH PROMOTION CENT
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