Quick tolclofos-methyl colloidal gold detection test strip and preparation method thereof
A technology of tolclofos-methyl and tolclofos-methyl, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of ELISA method application limitation, difficult to meet fast, online detection, and detection results are not intuitive enough, etc. Achieve the effect of showing the image of the results, facilitating promotion, and judging the image
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Embodiment 1
[0035] A colloidal gold rapid detection test strip for tolclofos-methyl, the test strip contains a liner 1 and a sample pad 2, a gold standard binding pad 3, a cellulose film 4 and a water-absorbent pad 7 arranged in sequence on the liner , the liner 1 is a tough material that does not absorb water, and the tough material is made of hard plastic strips, the gold-labeled binding pad 3 is adsorbed with a gold-labeled antibody for tolclofos-methyl, and the cellulose film There are invisible detection line 5 and invisible control line 6 on the 4, the invisible detection line 5 is printed with the carrier protein solution coupled with methyl tolclofos hapten, and the invisible control line 6 is printed with rabbit anti-mouse IgG antibody . The water-absorbing pad 7 is water-absorbing filter paper or oil filter paper with strong water-absorbing capacity. Tolclofos-methyl gold-labeled antibody is colloidal gold-labeled tolclofos-methyl monoclonal antibody. The carrier protein for c...
Embodiment 2
[0037] It is basically the same as Example 1, except that the tough material is made of non-absorbent hard paper strips, and the water-absorbing pad 7 is oil filter paper with strong water-absorbing capacity. The carrier protein for coupling tolclofos-methyl hapten is chicken ovalbumin OVA. The sample pad 2 is made of nylon film, polyvinylidene fluoride film and polyester film. Cellulose membranes are pure cellulose membranes.
Embodiment 3
[0039]It is basically the same as in Example 1, except that the tough material is made of hard plastic strips, and the water-absorbing pad 7 is water-absorbing filter paper with strong water-absorbing capacity. The carrier protein for coupling tolclofos-methyl hapten is human serum albumin HAS. The sample pad 2 is made of polyvinylidene fluoride film and polyester film. The cellulose film is a carboxylated cellulose film.
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