A femtosecond laser two-photon fluorescence biomicroscopic imaging system and its imaging method
A two-photon fluorescence and femtosecond laser technology, applied in fluorescence/phosphorescence, material excitation analysis, material analysis through optical means, etc., can solve the problems of two-photon fluorescence signal generation, femtosecond laser lock-out, failure to excite samples, etc. , to achieve the effect of low cost
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specific Embodiment approach 1
[0033] Specific implementation mode one: combine image 3 Describe this embodiment, a femtosecond laser two-photon fluorescence biomicroscopic imaging system, characterized in that: the system includes: tunable femtosecond laser source Tsunami (1), biological microscope (2), spectrometer (3), Photomultiplier tube (4), photodiode (5), data acquisition card (6), electric translation stage (7), electric translation stage controller (8), computer (9) and beam splitter (10); The biological microscope (2) includes a mirror M1 (11), a mirror M2 (12), a dichroic mirror (13), an emission filter (14), an objective lens (15) and a light collector (16);
[0034] The laser light generated by the tunable femtosecond laser source Tsunami (1) splits the laser light through a beam splitter (10), and enters the biological microscope (2) and spectrometer (3) respectively, and the spectrometer (3) is used to monitor the tunable The working state of the femtosecond laser source Tsunami (1), the l...
specific Embodiment approach 2
[0045] Specific embodiment two: this embodiment differs from specific embodiment one in that: the emission filter (14) is made of a band-pass filter and an infrared cut-off filter, and the band-pass filter is located near the dichroic mirror (13) On one side, the infrared cut-off filter is located on the side close to the photomultiplier tube (4).
specific Embodiment approach 3
[0046] Embodiment 3: The difference between this embodiment and Embodiment 1 or 2 is that the laser generated by the tunable femtosecond laser source Tsunami is an ultrashort pulse with a center wavelength of 800 nm, a repetition frequency of 82 MHz, and a pulse width of 50 fs Mode-locked laser.
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