Detection method of β-cryptoxanthin mono-cis, di-cis isomers and ketone oxidation products

Abstract

The invention relates to a detection method of beta-cryptoxanthin single-cis-form and two-cis-form isomer and ketone oxidation product, belonging to the technical field of the analysis. The detection method is characterized in that a YMC Carotenoid C30 chromatographic column is adopted, the flowing phase is 1.5% ammonium acetate water, methyl tert-butyl ether and methanol in a ratio of 5 / 25 / 70 and 1.5% ammonium acetate water, methyl tert-butyl ether and methanol in a ratio of 5 / 85 / 10, the flow rate is 0.6mL / min, the feeding amount is 20 micro liters, the column temperature is 25 DEG C, the detection wavelength is 200nm to 550nm, the beta-cryptoxanthin isomer and oxidation product can be remarkably separated, an APCI<+> positive ion mass spectrum is adopted, the flow rate of outflow components, entering a mass spectrometer, of the chromatographic column is 10 micro liters per minute, the scanning range is 80m / z to 1000m / z, the capillary voltage is 2500V, the drying gas is 5L, the amount of atomized gas is 20psi, the vaporization temperature is 300DEG C, the steam temperature is 400DEG C, and corona current is 4 micro amperes. The qualitative analysis of the beta-cryptoxanthin single-cis-form and two-cis-form isomer and ketone oxidation product is conducted according to an elution sequence, mass spectral information and spectrum information of a sample, and then the quantitative analysis can be conducted according to a peak area. The method has the advantages of rapidness, efficiency, reproducibility, high yield and the like.

Description

1. Technical field [0001] The invention relates to a method for detecting beta-cryptoxanthin mono-cis and di-cis isomers and ketone oxidation products, belonging to the field of analysis technology. 2. Background technology [0002] The chemical name of β-cryptoxanthin is 3-hydroxy-β-carotene. It is an oxygen-containing carotenoid with the function of VA precursor. It mainly comes from higher plants, fungi, cyanobacteria, etc., and is widely distributed in human blood, lungs, In tissues such as skin and oral mucosa. β-cryptoxanthin has a good ability to scavenge singlet oxygen and oxygen free radicals, so it has a significant effect on anti-cancer and anti-oxidation, especially has a unique curative effect on lung cancer caused by smoking. In addition, β-cryptoxanthin also plays an important role in preventing cardiovascular diseases and enhancing immune function. [0003] The conjugated double bonds and hydroxyl groups contained in the chemical structure of β-cryptoxanthi...

AI Technical Summary

Problems solved by technology

However, in practice, due to the close structure and polarity of carotenoids and their isomers, it is difficult to separate and detect them one by one

It has been reported that methanol and dichloromethane or methanol and methyl tert-butyl ether were used as the mixed mobile phase to separate cis-isomers of lutein and α, β-carotene, but no di-cis-isomers of β-cryptoxanthin were found. and related reports on the separation and detection of ketone oxides

Images