A method for rapidly inducing regenerated plants from the true leaves of Australian soybean

A technology for regenerating plants and Australian soybeans, which is applied to the field of rapidly inducing regenerated plants from true leaves of Australian soybeans, can solve the problems of delayed production, high production costs, and no seeds, and achieves the effects of healthy differentiation buds, short differentiation time, and strong root systems.

Active Publication Date: 2016-08-24
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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AI Technical Summary

Problems solved by technology

[0003] At present, high-quality Australian soybean seeds rely on imports. Domestic Australian soybean breeding is mainly based on sowing. There is no corresponding professional organization for seed harvesting and seedling cultivation. Not only is the production cost high, but also it is difficult to guarantee time and quantity, which limits the Australian soybean industry. formation and development
In addition, Australian soybeans are cool, humid, and sunny, avoiding heat and humidity. In my country, they are suitable for sowing in autumn and harvesting in late spring and early summer. However, the high temperature in autumn is not good for seedling cultivation and delays production.

Method used

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  • A method for rapidly inducing regenerated plants from the true leaves of Australian soybean

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Experimental program
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Embodiment 2

[0027] In this example, KT and IAA are used as the main auxin for callus induction differentiation and proliferation culture redifferentiation as controls, including the following steps:

[0028] 1. Disinfection of mature embryos: Select full-bodied Australian soybean seeds with intact seed coats, soak them in distilled water for 12 hours at room temperature, so that the seeds can fully absorb water and swell, remove the seed coats, and select complete seed embryos; on the ultra-clean workbench, Soak in 0.5% potassium permanganate solution for 6 minutes, rinse with sterile water 3 to 5 times, 3 minutes each time; then disinfect with 3.0% sodium hypochlorite solution for 8 minutes, rinse with sterile water 3 to 5 times, 5 minutes each time.

[0029] 2. Start culture: inoculate the mature embryos treated in step (1) into the start medium N 6 Cultured for 15-20 days. The sucrose in the medium is 30 g / L, the agar is 6 g / L, and the pH value is 5.8-5.9. During this period, the cul...

Embodiment 3

[0038] In this example, 1 / 2 N 6 No N spraying for rooting medium and transplanting 6 The macroelement mother solution is used as a contrast, comprising the following steps:

[0039] 1. Disinfection of mature embryos: Select full-bodied Australian soybean seeds with intact seed coats, soak them in distilled water for 12 hours at room temperature, so that the seeds can fully absorb water and swell, remove the seed coats, and select complete seed embryos; on the ultra-clean workbench, Soak in 0.5% potassium permanganate solution for 6 minutes, rinse with sterile water 3 to 5 times, 3 minutes each time; then disinfect with 3.0% sodium hypochlorite solution for 8 minutes, rinse with sterile water 3 to 5 times, 5 minutes each time.

[0040] 2. Start culture: inoculate the mature embryos treated in step (1) into the start medium N 6 Cultured for 15-20 days. The sucrose in the medium is 30 g / L, the agar is 6 g / L, and the pH value is 5.8-5.9. During this period, the culture tempera...

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Abstract

A method for rapidly inducing regenerated plants from the true leaves of Australian soybeans, relating to the rapid propagation technology of Australian soybean seedlings, in particular a method for generating regenerated plants through callus induction, including disinfection of mature embryos, initiation of culture, callus induction and Differentiation, proliferation culture and redifferentiation, rooting culture and seedling transplanting. The method is characterized in that the callus is induced by aseptic true leaves, and then adventitious buds are produced through differentiation of the callus, and then the regeneration plant is obtained. The method for quickly inducing and regenerating plants from the true leaves of Australian soybeans has low production cost, high induction rate, short differentiation time, and strong rooted seedlings. The acquisition rate of sterile seedlings was 93.6%, the healing rate was 68.9%, the bud differentiation rate was 84.7%, the rooting rate was 80.2%, and the transplanting survival rate was 87.1%. It can effectively solve the production demand of Australian soybean seedlings and carry out large-scale production.

Description

technical field [0001] The invention relates to a method for rapidly inducing and regenerating plants of Australian bean true leaves, and belongs to the technical field of plant seedling rapid propagation. Background technique [0002] Australian beans (Narrow-leafed Lupine ) belongs to the Fabaceae Papilionaceae lupin is an annual herb, native to the Mediterranean region of Europe, is a bean crop produced in Australia. Long-term consumption of Australian soybeans has the effects of lowering blood sugar index, suppressing appetite, promoting energy balance, lowering total cholesterol, lowering blood pressure, and improving gastrointestinal health; Australian soybeans are rich in protein and reasonable amino acid composition, and are used as an important food formula. Functional components and food additives are widely used in the food industry; Australian bean sprouts can be used as a vegetable supplement or substitute for soybeans and mung bean sprouts. Compared with soybe...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 高柱王小玲刘腾云余发新幸学俊
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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