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A method for rapid propagation of lupine "gallery" leaves combined with out-of-bottle rooting technology

An exogenous root and lupine technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low seed setting rate, difficulty in ensuring time and quantity, difficulty in inheriting good characters, etc., and achieve short differentiation time, The effect of efficient preservation of genetic traits

Inactive Publication Date: 2017-08-08
INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The lupine "gallery" is mainly propagated by seeds, but because lupine is a self-pollinating plant, the seed reproduction coefficient is low, and the seeds are mainly imported. The corresponding cost accounts for 30% of the production cost, and it is difficult to guarantee the time and quantity. The production of high-quality seedlings has become a bottleneck restricting the promotion of lupine "gallery" in my country
At present, the technology of tissue culture and rapid propagation of lupine "gallery" using imported seeds as explants is still not mature enough, and it is difficult to supply seedlings; "Single plant, because of its very low seed setting rate, it is difficult to pass on its excellent traits to future generations, and the promotion of improved varieties is seriously hindered

Method used

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  • A method for rapid propagation of lupine "gallery" leaves combined with out-of-bottle rooting technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] In this example, cytokinin ZT is not added to the callus induction and differentiation medium, and the callus induction culture is not cultured in the dark as a control, including the following steps:

[0024] 1. Leaf treatment: Cut off the young leaves 2 / 3 away from the tip, rinse with running water for 30 minutes, and cut them into 1cm-wide slices through the main vein. On the ultra-clean workbench, first disinfect with 75% alcohol for 10 seconds, rinse with sterile water twice; then use 10% NaOCl magnetic stirring for 5 minutes, rinse with sterile water for 5 times; then disinfect with 0.1% mercuric chloride solution for 3 minutes, Rinse 5 times with sterile water; finally blot the leaf surface moisture with sterile filter paper. Cut off the leaf edge, cut into 3mm×3mm blocks in the form of crosscutting the main vein, and make 2 to 3 incisions on the main vein.

[0025] 2. Callus induction: Inoculate the treated leaves on the medium MS+6-BA 0.5mg / L+ZT1.0mg / L+PVP 5.0...

Embodiment 2

[0034] In this example, different concentrations of 6-BA and NAA were added to the proliferation and redifferentiation medium as a control, including the following steps:

[0035] 1. Leaf treatment: Cut off the young leaves 2 / 3 away from the tip, rinse with running water for 30 minutes, and cut them into 1cm-wide slices through the main vein. On the ultra-clean workbench, first disinfect with 75% alcohol for 10 seconds, rinse with sterile water twice; then use 10% NaOCl magnetic stirring for 5 minutes, rinse with sterile water for 5 times; then disinfect with 0.1% mercuric chloride solution for 3 minutes, Rinse 5 times with sterile water; finally blot the leaf surface moisture with sterile filter paper. Cut off the leaf edge, cut into 3mm×3mm blocks in the form of crosscutting the main vein, and make 2 to 3 incisions on the main vein.

[0036] 2. Callus induction: Inoculate the treated leaves on the medium MS+6-BA 0.5mg / L+ZT1.0mg / L+PVP 5.0mg / L+VC 1.0mg / L with the back side do...

Embodiment 3

[0044] In this example, the concentration of rooting liquid and the soaking time are different during rooting culture outside the bottle, and the ratio of nutrient soil when transplanting and planting is different as a comparison, including the following steps:

[0045] 1. Leaf treatment: Cut off the young leaves 2 / 3 away from the tip, rinse with running water for 30 minutes, and cut them into 1cm-wide slices through the main vein. On the ultra-clean workbench, first disinfect with 75% alcohol for 10 seconds, rinse with sterile water twice; then use 10% NaOCl magnetic stirring for 5 minutes, rinse with sterile water for 5 times; then disinfect with 0.1% mercuric chloride solution for 3 minutes, Rinse 5 times with sterile water; finally blot the leaf surface moisture with sterile filter paper. Cut off the leaf edge, cut into 3mm×3mm blocks in the form of crosscutting the main vein, and make 2 to 3 incisions on the main vein.

[0046] 2. Callus induction: Inoculate the treated ...

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Abstract

The invention discloses a lupine 'gallery' leaf rapid propagation method achieved through an ex-vitro rooting technology and relates to a lupine 'gallery' seedling rapid propagation and good genetic character conservation technology, in particular to a method for producing regeneration plants through callus induction and ex-vitro rooting culture. The method includes leaf processing, callus induction, differentiation cultivation, multiplication culture, re-differentiation, ex-vitro rooting and transplantation and field planting. According to the method, calluses are induced through detected leaves, then adventitious buds are generated through callus differentiation, and then regeneration plants are acquired through ex-vitro rooting culture. By means of the method, the leaf pollution rate is smaller than 5%, the callus induction rate is 72.8%, the bud differentiation rate is 79.5%, the multiplication and re-differentiation rate is 83.6%, the ex-vitro rooting survival rate is 85.3%, and the transplantation survival rate is 92.5%. The production requirement of lupine 'gallery' seedlings is effectively met, the asexual propagation problem of good single plants is effectively solved, and the method can be used for large-scale production and efficient conservation of good genetic characters.

Description

technical field [0001] The invention relates to a method for rapidly inducing regeneration of lupine "gallery" leaves and belongs to the technical field of flower tissue culture. Background technique [0002] Lupine (Lupins polyphyllus Lindl) belongs to the Fabaceae Papilionaceae Lupin is a one-year to perennial herb, commonly known as Lupine flower, because it has a special meaning - China's mother flower, and has been doubly loved by people. "Gallery" is an early-flowering lupine variety cultivated on the market. It has pink, red, white, yellow, blue and mixed color series. The plant shape is compact, the leaves are lush, and the plant height is 40-50cm. It is a raw potted variety, and its appearance has given lupine the opportunity to enter ordinary households from garden applications. [0003] The lupine "gallery" is mainly propagated by seeds, but because lupine is a self-pollinating plant, the seed reproduction coefficient is low, and the seeds are mainly imported. Th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王小玲刘腾云高柱余发新幸学俊杨爱红刘淑娟肖亮
Owner INST OF BIOLOGICAL RESOURCES JIANGXI ACAD OF SCI
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