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Method for preparing retinal pigment epithelia by utilizing human marrow mesenchymal stem cells

A bone marrow mesenchymal and retinal pigment technology, applied in the biological field, can solve the problems of cell contamination, cell function detection, lack of RPE cytochrome particles, etc., and achieve the effect of short differentiation time and high differentiation efficiency

Inactive Publication Date: 2013-10-02
THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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Problems solved by technology

[0004] In recent years, in order to find autologous cells, some people try to induce the differentiation of human adipose-derived mesenchymal stem cells into RPE cells in vitro. The culture medium induces the differentiation of human adipose-derived mesenchymal stem cells. The cells induced by this method have the phenotype of RPE cells, but do not have the typical pigment granules of RPE cells, and the function of the cells has not yet been tested. In addition, centrifugation The obtained conditioned medium may still be mixed with primary cells, resulting in cell contamination

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  • Method for preparing retinal pigment epithelia by utilizing human marrow mesenchymal stem cells
  • Method for preparing retinal pigment epithelia by utilizing human marrow mesenchymal stem cells
  • Method for preparing retinal pigment epithelia by utilizing human marrow mesenchymal stem cells

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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with the embodiments and drawings, which are only for illustration and not limiting the scope of application of the present invention. The present invention is not limited to the following embodiments or examples, and any modifications and variations that do not violate the spirit of the present invention shall be included within the scope of the present invention.

[0026] In this example, the retinal pigment epithelial cells used in the non-contact co-culture are primary retinal pigment epithelial cells. Epithelial cells work better.

[0027] In this embodiment, the non-contact co-cultivation includes the following steps:

[0028] a. Inoculate human bone marrow mesenchymal stem cells in a cell culture well plate, add culture medium, place and cultivate until the cells adhere to the wall, and discard the culture medium in the well plate;

[0029] In this step, the human bone marrow mesenchymal ste...

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Abstract

The invention discloses a method for preparing retinal pigment epithelia by utilizing human marrow mesenchymal stem cells. According to the method, the human marrow mesenchymal stem cells are subjected to induced differentiation to form the retinal pigment epithelia by the non-contact coculture of the human marrow mesenchymal stem cells and the retinal pigment epithelia. Compared with the prior art, the method has the advantages that the human marrow mesenchymal stem cells are differentiated into the retinal pigment epithelia by the non-contact coculture of the human marrow mesenchymal stem cells and the retinal pigment epithelia, the differentiated retinal pigment epithelia have the important phenotype and physiological functions of the primary retinal pigment epithelia, differentiation time is short, differentiation efficiency is high, and the mixing and pollution of induction cells are avoided in the culture process.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for preparing retinal pigment epithelial cells using human bone marrow mesenchymal stem cells. Background technique [0002] Retinal degenerative diseases are a class of serious blinding diseases, mainly including Retinal pigment degeneration (RP for short) and age-related macular degeneration (AMD for short). The pathogenesis of such diseases is unknown, and the effective prevention and treatment methods are limited, which seriously endangers the quality of life of patients and causes hundreds of billions of huge losses worldwide every year. The pathological process of these diseases is caused by the degeneration and death of retinal pigment epithelial cells (Retinal pigment epithelium, referred to as RPE). Therefore, the use of RPE cells for replacement transplantation therapy has become an important means for the treatment of such diseases, but the source of RPE cells is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/071
Inventor 段平阴正勤徐海伟曾玉晓刘俊
Owner THE FIRST AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIVERSITY OF PLA
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