Immortalized cell line of human jugular paraganglioma and its application

A jugular bulb and paraganglion technology, which is applied in the biological and medical fields, can solve the problems of low cell yield, long experiment period, and inability to pass passage, and achieve the effect of simple cultivation requirements, short experiment period, and consistent background

Active Publication Date: 2022-03-29
SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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Problems solved by technology

These methods have the following disadvantages: (1) non-paraneuroma-derived cells lack specificity and cannot actually simulate the true phenotype of jugular bulbar paraganglioma; (2) primary tumor cells grow slowly, cannot be passaged, and are affected by patients. (3) The extraction and cultivation of primary cells are more complicated, the cell yield is low, the experiment cycle is longer, the genotype background of the cells is inconsistent, and a large number of non-tumor cells are mixed. cell

Method used

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  • Immortalized cell line of human jugular paraganglioma and its application
  • Immortalized cell line of human jugular paraganglioma and its application
  • Immortalized cell line of human jugular paraganglioma and its application

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Experimental program
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Effect test

Embodiment 1

[0110] The equipment and reagents required for the test are shown in Table 1 and Table 2 below.

[0111] Table 1. Instruments

[0112]

[0113] Table 2. Reagent Consumables

[0114]

[0115]

[0116] The technical solution includes the following steps:

[0117] 1. Screening and diagnosis of human glomus jugular tumor cells

[0118] 1.1 Patients with definite glomus jugular tumor were screened and diagnosed before operation, and glomus jugular paraganglioma was diagnosed pathologically during and after surgery.

[0119] Patient screening was confirmed by intracranial MRI, see figure 1 , Enhanced brain MRI showed obvious enhancement of the left jugular foramen, with mixed low signal, glomus jugular tumor performance.

[0120] Tumor tissue gene sequencing confirmed that there is a pathologically significant point mutation in the SDHB gene (EO7 exon c.649C>T, missense mutation), see figure 2 , is the sequencing result of the patient's tumor tissue.

[0121] Other c...

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Abstract

The invention provides a human jugular paraganglioma immortalized cell strain, the preservation number of which is: CGMCC NO.19664, and the cell strain is classified and named as a human jugular paraganglioma immortalized cell strain. The cell strain of the present invention is the first jugular bulb paraganglioma cell strain derived from human. There is a mutation of the SDHB gene, which can simulate the phenotype of jugular paraganglioma in the background of human SDHB gene mutation. The cell line is immortalized and can be cultured and subcultured in large quantities. The culture requirements are relatively simple, and a large number of experimental cells can be obtained in a short period of time. The overall experimental cycle is short. The genotype background of the obtained cells is consistent, free of impurities and cells, and is more suitable for basic experiments and promotion than primary cells.

Description

technical field [0001] The invention belongs to the technical field of biology and medical treatment, and in particular relates to an immortalized cell line of human jugular paraganglioma and application thereof. Background technique [0002] At present, the research on glomus jugular tumor is mainly limited to clinical research, and the basic research on tumor occurrence and development is greatly limited due to the lack of suitable cell lines. Previous studies have focused on silencing succinate dehydrogenase B (SDHB) using non-paraneuroma-derived cells or using primary tumor cells. These methods have the following disadvantages: (1) non-paraneuroma-derived cells lack specificity, and cannot actually simulate the true phenotype of jugular paraganglioma; (2) primary tumor cells grow slowly, cannot be passaged, and are affected by patients. (3) The extraction and cultivation of primary cells is more complicated, the cell yield is low, the experiment cycle is longer, the gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/867C12N5/10
CPCC12N15/86C12N5/0693C12N2510/04C12N2740/15043
Inventor 吴皓汪照炎王志刚陈洪赛
Owner SHANGHAI NINTH PEOPLES HOSPITAL SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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