A method of indentifying brucella natural infection or immunifaction for livestock

A Brucella, natural infection technology, applied in the field of proteomics, can solve the problems of human and healthy livestock threats, national and personal economic losses, inability to distinguish natural infection and immunization of livestock, etc.

Inactive Publication Date: 2014-11-26
ICDC CHINA CDC
View PDF1 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because traditional detection methods cannot distinguish between natural infection and immunization of livestock, if brucellosis sick animals are mistaken for immune livestock, the source of infect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method of indentifying brucella natural infection or immunifaction for livestock
  • A method of indentifying brucella natural infection or immunifaction for livestock
  • A method of indentifying brucella natural infection or immunifaction for livestock

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0029]The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the examples are all in accordance with conventional experimental conditions, such as Sambrook et al. Molecular cloning experiment manual (Sambrook J & Russell DW, Molecular cloning: a laboratory manual, 2001), or in accordance with the conditions suggested by the manufacturer's instructions.

[0030] Example Methods for identifying Brucella naturally infected or immunized livestock

[0031] According to the current state of application of animal vaccines in my country, the main way to prevent bovine brucellosis is to inoculate sheep No. 5 Brucella vaccine (M5) and swine No. 2 Brucella vaccine (S2), while cattle infection is mainly Brucella bovis. Therefore, through the differences in the presence of Omp31 and Bp26 in different species, it is possible to distinguish Brucella bovis from non-bovine at the serolog...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

A method of indentifying brucella natural infection or immunifaction for livestock is provided. According to the method, Omp31 protein or recombinant Omp31 protein containing a His label is adopted as interspecific difference identification protein, and Bp26 protein or recombinant Bp26 protein containing a His label is adopted as positive control protein. For livestock positive to a brucella infection serum SAT, the Bp26 protein or the recombinant Bp26 protein containing the His label reacts with serums infected by different strains of brucella, while the Omp31 protein or the recombinant Omp31 protein containing the His label only reacts with serums infected by brucella except the bovine brucella, and therefore the livestock which is positive to the SAT is naturally infected or is subjected to immunifaction. Through Western Blot detection, the two kinds of protein can identify the bovine brucella immune serums and non-bovine brucella immune serums, and can identify natural infection and immunifaction of bovine. The Omp31 protein and the Bp26 protein can be used for indentifying nonspecific agglutination reactions of other microorganisms with a brucella SAT antigen.

Description

technical field [0001] The present invention relates to proteomics and, in particular, to a method for identifying Brucella in naturally infected or immunized livestock. Background technique [0002] Brucella (Brucella for short) is a Gram-negative facultative intracellular parasite and the pathogen of Brucellosis (Brucellosis for short). Brucellosis is a zoonotic disease, which belongs to Class B infectious diseases in my country's Infectious Disease Prevention and Control Law. Internationally, brucellosis is classified as a Class B infectious disease, and it is prevalent all over the world. Especially after 2000, the epidemic situation of brucellosis has risen sharply, causing serious health damage and economic loss to the people. [0003] Currently, the gold standard for the diagnosis of Brucella is the isolation and culture of bacteria. However, the culture of Brucella is difficult, the cycle is long, and the isolation rate is low. The most commonly used serological de...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/68G01N33/531
CPCG01N33/56911G01N33/68G01N2333/23
Inventor 崔步云姜海杨星雅田国忠赵鸿雁朴东日
Owner ICDC CHINA CDC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap