Bacillus megaterium and application thereof in aquaculture

A Bacillus megaterium and expanded culture technology, applied in the field of functional microorganism screening, can solve problems such as excessive nitrogen content in water, destruction of aquaculture ecological environment, frequent fish and shrimp diseases, etc., to improve utilization, reduce water pollution, and degrade effects. significant effect

Active Publication Date: 2014-11-26
QINGDAO VLAND BIOTECH GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] my country's aquaculture industry has developed rapidly in recent years, and the scale of intensive and high-density farming has been expanding day by day. Concentrated bait feeding has caused a large amount of residual bait, feces, and dead animals and plants to accumulate

Method used

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  • Bacillus megaterium and application thereof in aquaculture

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Isolation, screening and identification of embodiment 1 bacterial strain

[0013] 1. Sample

[0014] Water samples taken from the wastewater treatment tower of salmon recirculating aquaculture in Yantai Oriental Ocean Group;

[0015] 2. Heterotrophic nitrification medium:

[0016] name

Weight (g)

NaNO 2

0.5

sodium succinate

5.62

K 2 HPO 4

5.0

MgSO 4 ·7H 2 o

2.5

NaCl

2.5

FeSO 4 ·7H 2 o

0.05

MnSO 4 4H 2 o

0.05

h 2 o

1000

[0017] 3. Screening method

[0018] The applicant used the gradient dilution method to dilute the water sample to an appropriate concentration; take 0.1mL of the diluted water sample and spread it evenly on the surface of the above-mentioned heterotrophic nitrification medium, put it in a constant temperature incubator, and cultivate it at 30°C for 2 to 3 days. The inoculation loop picks up a single colony on the culture med...

Embodiment 2

[0033] Example 2 Bacillus megaterium YX-7 treatment of aquaculture water experiment

[0034] 1. Preparation of bacteria powder

[0035] Firstly, Bacillus megaterium YX-7 is activated, expanded and cultivated, then fermented in liquid state and spray-dried to make bacteria powder. The content of Bacillus megaterium is about 10 billion / g.

[0036] 2. Experimental location: small shed shrimp breeding pond in Rudong, Jiangsu

[0037] Three aquaculture ponds with similar water quality indicators were selected as three parallels, and the water bodies in the aquaculture ponds were separated by plastic sheets, half of the water bodies were used as the experimental group, and the other half of the water bodies were used as the control group. Separately detect NO in each group of water bodies 2 - - the initial concentration of N; then add 10ppm of the above-mentioned Bacillus megaterium YX-7 bacteria powder to the water body of the experimental group, and the control group does not a...

Embodiment 3

[0043] Embodiment 3: the impact of Bacillus megaterium YX-7 on feed-to-meat ratio of aquatic animals

[0044] Experimental location: small shed shrimp culture pond in Rudong, Jiangsu;

[0045] Select a pond with normal water quality and healthy prawns. In the pond, build 6 enclosures with a length, width, and height of 4m×4m×2m. The same amount of water in the pond is injected into the enclosure. With an equal amount of aeration stones, the aeration volume can be adjusted evenly and consistently. 3 were randomly selected as the experimental group, and the other 3 as the control group. Each group was fed with 1000 healthy Penaeus vannamei. Before feeding, 500 shrimps were randomly selected from each group for weighing, and the average value was taken for each of the three experimental groups and the control group.

[0046] The feed of Penaeus vannamei is commercially available prawn compound feed, wherein the feed of the experimental group is added with Bacillus megaterium YX...

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Abstract

The present invention discloses a new strain of Bacillus megaterium YX-7, which is screened from a water sample of a salmon circulating aquaculture sewage treating tower, and has heterotrophic and nitrification performances, and the preservation number is CCTCC (China Center For Type Culture Collection) NO:M2014275. The strain has significant degradation effect on NO2-N in water, 48h after the treatment, the NO2-N removal rate reaches 91.15%, and the Bacillus megaterium YX-7 can be used for aquaculture water and domestic sewage treatment, and can reduce water pollution; in addition, the Bacillus megaterium YX-7 can effectively improve the feed utilization rate of aquatic animals, the feed meat ratio of penaeus vannamei boone of an experimental group is decreased by 8% compared that of a control group, so that the Bacillus megaterium YX-7 can be used as feed additives for application in aquatic feeds, and is conducive to the improvement of farmer economic benefits and wide in application prospect.

Description

technical field [0001] The invention relates to the technical field of functional microorganism screening, in particular to a strain of Bacillus megaterium and its application in aquaculture. Background technique [0002] my country's aquaculture industry has developed rapidly in recent years, and the scale of intensive and high-density farming has been expanding day by day. Concentrated bait feeding has caused a large amount of residual bait, feces, and dead animals and plants to accumulate in the aquaculture water, resulting in a serious excess of nitrogen content in the water body and aggravated eutrophication. , causing the destruction of the breeding ecological environment, frequent occurrence of fish and shrimp diseases, and ultimately causing huge economic losses. The nitrification process converts NO 2 - -N converted to NO 3 - -N is an important part of the nitrogen cycle in nature. With the development of high-density aquaculture, the amount of feed continues to...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A23K1/16C12R1/11A23K10/18A23K50/80
Inventor 武玉强苟万里刘广
Owner QINGDAO VLAND BIOTECH GRP
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