A targeted gene nanoparticle with anti-endometriosis and its preparation

An endometrial and gene-targeting technology, which is used in medical preparations with non-active ingredients, gene therapy, sexual diseases, etc., to achieve the effects of increasing transfection efficiency, improving curative effect, and reducing toxic and side effects

Active Publication Date: 2017-06-30
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these angiogenesis inhibitors can inhibit the implantation and growth of normal proliferative endometrium in model animals to varying degrees, the therapeutic effects, especially the long-term therapeutic effects, on the modeled animal atopic lesions remain to be studied.

Method used

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  • A targeted gene nanoparticle with anti-endometriosis and its preparation
  • A targeted gene nanoparticle with anti-endometriosis and its preparation
  • A targeted gene nanoparticle with anti-endometriosis and its preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Preparation of siRNA-loaded anti-endometriosis gene nanoparticles

[0025] (1) Preparation of siRNA-loaded anti-endometriosis gene nanoparticles

[0026] CSO was modified with PEI by periodate method. Weigh 1.2g of CSO and 0.06g of sodium periodate, and dissolve them in 180mL and 60mL of acetic acid buffer solution of pH 4.5 respectively. Before the reaction, the two solutions are nitrogen-gassed to remove dissolved oxygen. Then the sodium periodate solution was added dropwise into the CSO solution, and the mixture was stirred and reacted at 400 rpm for 48 hours under an ice bath at 4°C. After the reaction is over, add a certain amount of ethylene glycol to terminate the reaction. Put the final reaction solution in a dialysis bag and dialyze for 8 hours to remove impurities, and the dialysate is ready for use. Another appropriate amount of PEI was weighed and dissolved in water, slowly added to the dialysate, and stirred for 48 hours. After the reaction, ...

Embodiment 2

[0029] Example 2: Preparation of siRNA-loaded anti-endometriosis gene nanoparticles

[0030] (1) Preparation of siRNA-loaded anti-endometriosis gene nanoparticles

[0031]CSO was modified with PEI by periodate method. Weigh 1.2g of CSO and 0.06g of sodium periodate, and dissolve them in 180mL and 60mL of acetic acid buffer solution of pH 4.5 respectively. Before the reaction, the two solutions are nitrogen-gassed to remove dissolved oxygen. Then the sodium periodate solution was added dropwise into the CSO solution, and the mixture was stirred and reacted at 400 rpm for 48 hours under an ice bath at 4°C. After the reaction is over, add a certain amount of ethylene glycol to terminate the reaction. Put the final reaction solution in a dialysis bag and dialyze for 8 hours to remove impurities, and the dialysate is ready for use. Another appropriate amount of PEI was weighed and dissolved in water, slowly added to the dialysate, and stirred for 48 hours. After the reaction, a...

Embodiment 3

[0034] Example 3: Preparation of siRNA-loaded anti-endometriosis gene nanoparticles

[0035] 1) Preparation of siRNA-loaded anti-endometriosis gene nanoparticles

[0036] CSO was modified with PEI by periodate method. Weigh 1.2g of CSO and 0.06g of sodium periodate, and dissolve them in 180mL and 60mL of acetic acid buffer solution of pH 4.5 respectively. Before the reaction, the two solutions are nitrogen-gassed to remove dissolved oxygen. Then the sodium periodate solution was added dropwise into the CSO solution, and the mixture was stirred and reacted at 400 rpm for 48 hours under an ice bath at 4°C. After the reaction is over, add a certain amount of ethylene glycol to terminate the reaction. Put the final reaction solution in a dialysis bag and dialyze for 8 hours to remove impurities, and the dialysate is ready for use. Another appropriate amount of PEI was weighed and dissolved in water, slowly added to the dialysate, and stirred for 48 hours. After the reaction, a...

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Abstract

The invention provides target gene nanoparticle capable of resisting endometriosis, which is loaded with micromolecule interference RNA gene medicines, the nanoparticle materials are composed of chitosan oligosaccharide, polyethyleneimine and hyaluronic acid, wherein proportion of chitosan oligosaccharide to polyethyleneimine is 1:1, hyaluronic acid accounts for 5-30% of total nanoparticle proportion, and the proportion of phosphorus-containing mole ratio in gene medicine to the nitrogen containing mole ratio in the nanoparticles is 0.1-16. According to the invention, through the high-molecular nano-material, by using acceptor-ligand characteristic, the gene medicine in the cell can be encapsulated, intake of gene medicine by endometriosis cell can be increased, transfection efficiency of target tissue is increased, intake of medicine by endometriosis cell is increased, distribution of medicine in normal tissue or cell is reduced, toxic and side effect of medicine is reduced, curative effect of the gene medicine for resisting endometriosis is increased, and the gene medicine can be used for resisting endometriosis.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to the preparation of gene nanoparticles and the application of gene nanoparticles in anti-endometriosis. Background technique [0002] Endometriosis (referred to as endometriosis) is a common disease in women of childbearing age, and its incidence rate is on the rise. The incidence rate of women of reproductive age can be as high as 15%. Its clinical features are mainly pain, mass and infertility. Although there are many ways to treat endometriosis, no matter what kind of treatment, the recurrence rate within 5 years is more than 40%, and 1% of endometriosis patients may become malignant into a special type of ovarian cancer. The benign nature of the disease and the potential of malignant biological behaviors such as invasion, metastasis, and recurrence make it an intractable disease. [0003] At present, the researches on the treatment of endometriosis involving anti-angiogenic drugs are a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K9/14A61K47/36A61K47/32A61P15/00
Inventor 赵梦丹黄荷凤陈凤英郑彩虹应伟雯严晶晶宋瑶真
Owner ZHEJIANG UNIV
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