A method for detecting antiviral drug residues in livestock and poultry hair

A technology of residue and hair, applied in the field of qualitative and quantitative detection of amantadine, rimantadine and morpholinidine residues in livestock and poultry hair, can solve the problems of low detection limit and so on

Active Publication Date: 2016-01-20
INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0032] (3) Low detection limit in complex and dirty matrices

Method used

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  • A method for detecting antiviral drug residues in livestock and poultry hair
  • A method for detecting antiviral drug residues in livestock and poultry hair
  • A method for detecting antiviral drug residues in livestock and poultry hair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Wash the pig hair with tap water to remove visible impurities, ultrasonicate the hair in 1% SDS+5% Tween 80+pure water (1:1:1) for 15 minutes, rinse it with distilled water, and dry it with filter paper; dry the hair at 50°C, put Store in a desiccator.

[0104] Cut into 1-2mm pieces with scissors, weigh 0.5g of hair into a 50mL centrifuge tube, add 50.0μL of D15-amantadine standard working solution, 5mL of 0.2mol / L HCI solution, mix well, digest at 65°C for 2 hours, take out, Add 8 mL of 2.0% trichloroacetic acid aqueous solution + acetonitrile (1:2), vortex for 30 s, add 2 g of sodium chloride after ultrasonic extraction for 40 min, vortex for 2 min, centrifuge at 5000 r / min for 10 min, and take the supernatant in another In the tube, dry it with nitrogen at 60°C, add 3mL of 2% formic acid aqueous solution to dissolve, and load the solution into the MCX column that has been activated with 3mL of methanol and 3mL of water, and then rinse with 3mL of 2% formic acid aqueo...

Embodiment 2

[0109] Wash the chicken feathers with tap water to remove visible impurities, sonicate the hair in 1% SDS+5% Tween 80+pure water (1:1:1) for 15 minutes, rinse it with distilled water, and dry it with filter paper; dry the hair at 50°C and let it dry spare in the device.

[0110] Cut into 1-2mm pieces with scissors, weigh 0.5g of hair into a 50mL centrifuge tube, add 50.0μL of D15-amantadine standard working solution, 5mL of 0.2mol / L HCI solution, mix well, digest at 65°C for 2 hours, take out, Add 8 mL of 2.0% trichloroacetic acid aqueous solution + acetonitrile (1:2), vortex for 30 s, add 2 g of sodium chloride after ultrasonic extraction for 40 min, vortex for 2 min, centrifuge at 5000 r / min for 10 min, and take the supernatant in another In the tube, dry it with nitrogen at 60°C, add 3mL of 2% formic acid aqueous solution to dissolve, and load the solution into the MCX column that has been activated with 3mL of methanol and 3mL of water, and then rinse with 3mL of 2% formic...

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Abstract

The invention relates to a method, which serially connects ultrahigh performance liquid chromatography (UPLC)-electrospray ionization (ESI) with mass spectrum (MS) to detect the residual quantity of amantadine, rimantadine, and moroxydine in hairs of livestock and poultry.

Description

technical field [0001] The invention belongs to the field of detection of drug residues, in particular to a method for qualitatively and quantitatively detecting residues of amantadine, rimantadine and morpholinidine in livestock and poultry hair. Background technique [0002] Amantadine is an amino derivative of artificially synthesized saturated tricyclodecane, which is used for the prevention and early treatment of Asian influenza A virus, and also for the treatment of neurological disorders caused by Parkinson's disease and the prevention of common viral diseases in animals such as bird flu etc. Amantadine is an analogue of amantadine, which is also used to prevent and treat type A influenza virus infection. Its clinical curative effect is better than that of amantadine, and its adverse reactions are less than that of amantadine. Vialin, common name: morpholine guanidine, morpholine guanidine has inhibitory effects on a variety of viruses (including influenza A H1N1, in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06
Inventor 罗林广胡丽芳张大文廖且根霍俊宏
Owner INST OF AGRI PROD QUALITY SAFETY & STANDARD JIANGXI ACAD OF AGRI SCI
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