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Lacquer tree polyphenol extract capable of inhibiting activity of tyrosinase and preparation method thereof

A tyrosinase and extract technology, applied in the field of sumac polyphenol extract and preparation thereof, can solve the problems of unutilized branches and stems, weak utilization of lacquer seeds, blank research and development, etc. Superficial, anti-decomposition, preventive and therapeutic effects of sedative diseases

Inactive Publication Date: 2014-11-19
INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the lacquer tree industry mainly focuses on the production and development of natural lacquer coatings, and the utilization of lacquer seeds is also relatively weak. In addition, in the process of growth and utilization of lacquer trees, a large number of branches and stems have not been utilized. In order to expand the scale of the lacquer tree industry Therefore, it is of guiding significance for the development and utilization of sumac resources to carry out the extraction, preparation and biological activity research of sumac polyphenols.
The known domestic and foreign literature on sumac polyphenols mainly focuses on anti-tumor, antibacterial and anti-oxidation methods, and the research and development of sumac polyphenols inhibiting tyrosinase activity is still blank

Method used

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  • Lacquer tree polyphenol extract capable of inhibiting activity of tyrosinase and preparation method thereof
  • Lacquer tree polyphenol extract capable of inhibiting activity of tyrosinase and preparation method thereof
  • Lacquer tree polyphenol extract capable of inhibiting activity of tyrosinase and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Analytical method of sumac polyphenols

[0037] Accurately weigh 20 mg of gallic acid standard substance, add distilled water to dissolve and set the volume to 200 mL to obtain a standard solution of 0.1 mg / mL standard substance. Pipette 0, 0.2, 0.4, 0.6, 0.8, and 1 mL of 0.1 mg / mL standard solution into a 10 mL volumetric flask, add 1 mL of FC reagent (0.2 mol / L) and 2 mL of 15% Na2CO3 solution, and set Make up to the mark and react at 25°C for 2h. Absorbance was measured at 760nm. Draw a standard curve of absorbance versus concentration and fit a linear regression equation. Regression equation y=0.06895x+0.0465, correlation coefficient R=0.99985. Gallic acid has a good linear relationship in the concentration range of 2-10ug / mL.

Embodiment 2

[0038] Embodiment 2 HPLC analysis

[0039]The chromatographic column is filled with octadecyl chemically bonded stationary phase, that is, RP-18 endcapped (250mm×4.6mm, 5μm); the UV detection wavelength is 280 and 360nm, room temperature; the mobile phase is methanol with a volume ratio of 65:35 -0.5% acetic acid aqueous solution, the flow rate is 1mL / min, and the resolution between each standard is required to be greater than 1.5. Weigh the standard substances of chrysanthemum, fisetin, butacein and thiamethrin respectively, accurate to 0.00001g, add methanol respectively to make a 0.5mg / mL solution, and then dilute to a 50μg / mL standard solution. Draw 20 μL, 16 μL, 12 μL, 8 μL and 4 μL of each standard solution, inject the sample, calculate the peak area, establish the relationship between the peak area of ​​chrysanthemum, fisetin, butacein and thiamethrin and the sample amount (μg) respectively, and determine the regression equation and draw the corresponding standard curv...

Embodiment 3

[0041] Take 500g lacquer wood as raw material, after drying and crushing to remove slag, add 95% ethanol solution, extract at room temperature for 10 hours, extract 3 times, filter, combine filtrates, and remove solvent by rotary evaporation under reduced pressure at 60°C, water phase After lyophilization, 25 g of the crude extract was obtained, the polyphenol content was 35%, and the inhibition rate was 30%. Then add the crude extract of sumac polyphenols into distilled water at a mass ratio of 1:5 (g:mL), stir to dissolve, add petroleum ether for extraction 3 times, separate, evaporate under reduced pressure at 40°C to remove the solvent, and obtain petroleum ether extract; The aqueous layer was further extracted 5 times with ethyl acetate, separated, evaporated under reduced pressure at 40°C to remove the solvent, and dried to obtain the ethyl acetate extract phase. Add the ethyl acetate phase to 10% ethanol aqueous solution at a mass ratio of 1:5~1:10 (g:mL), dissolve it b...

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Abstract

The invention relates to a lacquer tree polyphenol extract capable of inhibiting the activity of tyrosinase and a preparation method thereof, belonging to the field of development and application of natural pharmaceutical chemistry and cosmetics. The wood of a lacquer tree is used as a raw material, and isolation, extraction, macroporous resin purification and drying are carried out so as to obtain the lacquer tree polyphenol extract. The lacquer tree polyphenol extract prepared in the invention has high content and an obvious inhibiting effect; the content of lacquer tree polyphenol is greater than 60%, and the inhibition rate on the activity of tyrosinase is greater than 60%. The raw material used in the invention is natural and nontoxic, so lacquer tree polyphenol has good commercial prospects when applied to development of a natural tyrosinase inhibitor for preparation of whitening cosmetics and for prevention and treatment of hyperpigmentation diseases caused by excess melanin in the human body.

Description

technical field [0001] The invention relates to a sumac polyphenol extract with activity of inhibiting tyrosinase and a preparation method thereof, belonging to the technical fields of natural medicine and cosmetics. Background technique [0002] Sumac (Rhus verniciflua stokes) is a deciduous tree of the Anacardiaceae genus Toxicodendron. Sumac is an important special-purpose economic forest in my country. Raw lacquer is a natural resin coating and is known as the "king of coatings". It is also an excellent medicinal wood. The leaves, flowers, roots, bark, fruits, dried lacquer and wood core of sumac tree can be used as medicine, which has the effects of relieving cough, eliminating addiction, stimulating menstrual flow, killing insects, and reducing swelling. The polyphenolic compound in the part is the main active substance following urushiol and urushiol, and a large number of studies have shown that it has excellent antioxidant, anti-tumor, antibacterial and immunomodulato...

Claims

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Application Information

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IPC IPC(8): A61K36/22A61K8/97A61P17/00A61Q19/02
Inventor 王成章陈虹霞叶建中
Owner INST OF CHEM IND OF FOREST PROD CHINESE ACAD OF FORESTRY
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