Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method of forming skeletal muscle cell by induction differentiation by utilization of pig dedifferentiated adipocyte

A technology for skeletal muscle cells and adipocytes is applied in the field of using pig dedifferentiated adipocytes to induce differentiation into skeletal muscle cells, which can solve the problems of lack of biological characteristics and functions of pig DFAT cells, no disclosure of DFAT cells, and no experimental data provided. To achieve the effect of simple and convenient induction procedure, convenient sampling and rich sources

Inactive Publication Date: 2014-10-29
HUAZHONG AGRICULTURAL UNIVERSITY
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The patent application literature related to the present invention is the method reported by the application number 201110002340.4 (name of invention: a culture method for rapid dedifferentiation of porcine mature adipocytes into preadipocytes). Mature adipocytes were isolated from tissues, and porcine DFAT cells were obtained through the "ceiling" culture method. This document discloses the method and path of using DFAT cells as preadipocytes, but only discloses the separation method for obtaining porcine DFAT cells, and does not The obtained porcine DFAT cells were studied and verified for their biological characteristics and functions, and no relevant experimental data was provided to support the evidence that the isolated DFAT cells were preadipocytes, nor were they disclosed in the examples of the patent publication. Information on the biological identification of isolated DFAT cells, especially without any experimental support and results for the differentiation of porcine DFAT cells into myogenic direction

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method of forming skeletal muscle cell by induction differentiation by utilization of pig dedifferentiated adipocyte
  • Method of forming skeletal muscle cell by induction differentiation by utilization of pig dedifferentiated adipocyte
  • Method of forming skeletal muscle cell by induction differentiation by utilization of pig dedifferentiated adipocyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] 1. Isolation and purification of porcine mature adipocytes and culture by "ceiling" method

[0054] (1) Separation and purification of pig mature adipocytes: Take 5-day-old "Landrace" boars (or "Landrace boars" for short, provided by the Experimental Breeding Pig Farm of Huazhong Agricultural University, which is a conventional variety), and treat them with 75% alcohol. After the whole body of the pig was sterilized twice, it was sacrificed by exsanguination from the carotid artery, and the blood was drained as much as possible. Subsequently, the whole body of the piglet was wiped with alcohol cotton containing 75% alcohol for disinfection. Move the piglet into the ultra-clean workbench, use sterilized scissors and tweezers under aseptic conditions, take the subcutaneous fat of the back of the piglet and place it in a place containing 1% double antibody (anti-penicillin-streptomycin, purchased from Hangzhou Jinuo Biomedical Technology Co., Ltd. ) phosphate-buffered sal...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of animal cell culturing, and particularly relates to a method of forming a skeletal muscle cell by induction differentiation by utilization of a pig dedifferentiated adipocyte. A mature adipocyte of pig subcutaneous adipose tissues is separated by a collagenase digestion method, and is dedifferentiated to form the dedifferentiated adipocyte (DFAT cell). The DFAT cell has functions similar to that of mesenchymal stem cells (MSCs) and can be efficiently multiplicated in vitro and can introduce multi-directional differentiation. In addition, by utilization of induction of the galectin-1 protein in vitro, the pig DFAT cell is dedifferentiated to form a multinucleate cell capable of expressing a skeletal muscle cell specific marker molecule.

Description

technical field [0001] The invention belongs to the technical field of in vitro culture of animal cells, and in particular relates to a method for inducing differentiation of porcine dedifferentiated adipocytes (DFAT) to form skeletal muscle cells. Background technique [0002] Pig muscle tissue is an important production trait in pig production. The skeletal muscle cells that form muscle tissue are multinucleated cells formed from mesoderm stem cells after finalization and differentiation. Unfortunately, no porcine-derived myoblast or stem cell lines have been established for the study of stem cell commitment and differentiation into skeletal muscle cells. Mesenchymal stem cells (mesenchymal stem cells, referred to as MSCs, the same below) and satellite cells derived from adult tissues are commonly used models for myogenic differentiation of primary stem cells (Dezawa et al2006). [0003] However, due to the small number of satellite cells isolated from muscle tissue in v...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/077
Inventor 彭健彭学武
Owner HUAZHONG AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com