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DL tyrosine chiral resolution and on-line detection method based on functional nano channel arrays

A nano-channel and chiral separation technology, applied in the field of materials, can solve problems such as embryo teratogenicity, achieve good application prospects, and simple separation and detection processes.

Inactive Publication Date: 2014-10-29
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Later, after research, it was found that the R body of the reaction stopper has a sedative effect, but the S-enantiomer has a strong teratogenic effect on the embryo.

Method used

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  • DL tyrosine chiral resolution and on-line detection method based on functional nano channel arrays
  • DL tyrosine chiral resolution and on-line detection method based on functional nano channel arrays
  • DL tyrosine chiral resolution and on-line detection method based on functional nano channel arrays

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: Preparation of gold nanochannel membrane

[0025] Gold was deposited on a 50 nm porous polycarbonate (PC) film by electroless deposition. Immerse the PC membrane in anhydrous methanol for 5 minutes to wash away the impurities adsorbed on the base membrane; then immerse the cleaned PC membrane in 0.025mol / L SnCl 2 The solution was placed in a shaker at room temperature for 45min at a speed of 90 rpm to make the Sn 2+ Uniformly adsorbed on the surface of the basement membrane and membrane pores, removed and rinsed with methanol for 3 times; 2 The membrane was immersed in 0.029mol / L Ag(NH 3 ) 2 + In the solution for 10 minutes, take it out and wash it with methanol for 3 times, and wash it with water for 3 times, and the immersion concentration is 7.9×10 -4 mol / L sodium gold sulfite deposition solution (pH=10.00). After electroless gold plating at 4°C for 3 hours, take it out and wash it with water for 3 times, and then use 25% HNO 3 Soak for 12 hours ...

Embodiment 2

[0026] Example 2: Modification of L-cysteine

[0027] The prepared Au nanochannel membrane was immersed in 1mmol / L L-cysteine ​​solution for 24h, and L-cysteine ​​was self-assembled on the Au nanochannel membrane through the gold-sulfur bond. After modification, the The membrane was taken out and washed three times with ultrapure water, dried at room temperature, and set aside.

[0028] figure 2 It is the XPS diagram of the gold nanochannel membrane modified with L-cysteine. The characteristic element sulfur can be seen from the figure, indicating that L-cysteine ​​has been successfully modified on the gold nanochannel membrane.

Embodiment 3

[0029] Example 3: Chiral resolution of DL tyrosine and its effect determination

[0030] according to figure 1 As shown, the U-shaped pool is used as the device for the chiral separation of DL tyrosine. The U-shaped pool is divided into two parts: the sampling pool and the separation pool. The gold nanochannel membrane is placed between the sampling pool and the separation pool. The transmission area is 0.196cm 2 .

[0031] Add 1.0×10 -4 mol / L D-tyrosine, 1.0×10 -4 mol / L L-tyrosine 5mL each, add 10mL water to the separation pool, detect the amount of D-tyrosine and L-tyrosine in the separation pool with a polarimeter at intervals, and draw the concentration of the substance in the osmosis pool with time. The relationship between the resulting straight line slope is defined as the resolution of the two analytes.

[0032] image 3 It is the case of DL tyrosine enantiomer passing through the gold membrane, where A is the concentration and time relationship graph of DL tyros...

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Abstract

The invention discloses a DL tyrosine chiral resolution and on-line detection method based on functional nano channel arrays in association with surface enhanced Raman spectrum. By using a polycarbonate film as a base film, a chemical deposition process is utilized to prepare the Au nano channel film with the diameter of 25nm or so, L-cysteine is self-assembled to the Au nano channel pore wall through the Au-S covalent bond to form a chiral selective identification film with L-cysteine on the surface. The channel subjected to chiral selective identification has a different effect on the DL tyrosine, so that the DL tyrosine permeation rate of the modification film is different, thereby separating the D-tyrosine from the L-tyrosine. By utilizing the difference of the SERS characteristic peak of the DL tyrosine and the very low detection limit, the object in the separation tank can be detected instantly. The invention provides a novel convenient method for separating and detecting the DL tyrosine which is hard to resolve.

Description

technical field [0001] The invention relates to the field of materials and separation technology, and is a molecular-level membrane separation and detection technology based on nanochannel membrane materials, specifically a method for separating and detecting tyrosine enantiomers based on nanochannels combined with surface-enhanced Raman spectroscopy. Background technique [0002] Nano science and technology is the product of the cross-combination of modern science and technology, and is the frontier of modern scientific research. Its research objects involve many fields, and many new research topics and research directions have emerged. Nanochannel technology, as one of the important contents and a new growth point of bionanotechnology research, provides a new means for the effective separation and detection of biological components. The gold nanochannel membrane has the characteristics of large specific surface area and controllable size of the nanochannel, which makes the...

Claims

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Application Information

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IPC IPC(8): C07C229/36C07C227/34G01N21/65
Inventor 黄杉生谢利柳悦杨乐乐马腾飞
Owner SHANGHAI NORMAL UNIVERSITY
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