Culture method for rapid propagation of cotinus coggygria 'royal purple'

A cultivation method and proliferation cultivation technology, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems not involved in the intermittent immersion micro-propagation technology of Cotinus sinensis, achieve good plant regeneration effect, reduce accumulation, The effect of improving quality

Inactive Publication Date: 2014-10-29
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technical content disclosed in the above-mentioned patent does not involve the intermittent immersion micro-propagation technology of Red Chin

Method used

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  • Culture method for rapid propagation of cotinus coggygria 'royal purple'
  • Culture method for rapid propagation of cotinus coggygria 'royal purple'
  • Culture method for rapid propagation of cotinus coggygria 'royal purple'

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1) Establishment of sterile system and preparation of multiplication materials

[0043] Collect the young stems with joints of Rhizoma sagitta in the same year, brush the dirt attached to the surface of the explants with detergent water, and rinse them under tap water for 30 minutes. Cut into 2.0-3.0cm long stems with 1-2 nodes; soak in 75% alcohol for 30s on a clean bench, and then use 0.1% HgCl 2 Sterilize for 6 minutes, rinse with sterile water 4 times. Inoculated in the addition of 2.0g·L -1 MS basic medium of polyvinylpyrrolidone PVP (Murashige and Skoog, 1962) + 30g L -1 Sucrose+5.5g·L -1 On agar (pH 6.0); after about 10 days of culture and observation, transfer sterile and well-growing stem segments into MS+6-BA0.1mg·L -1 +NAA0.4mg·L -1 +30g·L -1 Sucrose+5.5g·L -1 Axillary buds were induced in agar medium (pH 6.0), and when the axillary buds germinated and grew to 2.0 cm, they were cut and inserted into MS+6-BA 0.8mg L -1 +NAA0.4mg·L -1 +30g·L -1 Sucros...

Embodiment 2

[0055] 1) Establishment of sterile system and preparation of multiplication materials

[0056] Collect young young stems with joints of Rhizoma Americana, scrub the dirt attached to the surface of the explants with detergent water, and rinse them under tap water for 40 minutes. Cut into 2.0-3.0cm long stems with 1-2 nodes; soak in 75% alcohol for 30s on a clean bench, and then use 0.1% HgCl 2 Sterilize for 6 minutes, rinse with sterile water 4 times. Inoculated in the addition of 2.0g·L -1 MS basic medium of polyvinylpyrrolidone PVP (Murashige and Skoog, 1962) + 30g L -1 Sucrose+5.5g·L -1 on agar (pH 6.0); after 10 days of culture and observation, transfer sterile and well-growing stem segments into MS+6-BA0.1mg L -1 +NAA0.4mg·L -1 +30g·L -1 Sucrose+5.5g·L -1 Axillary buds were induced in agar medium (pH 6.0), and when the axillary buds germinated and grew to 2.0 cm, they were cut and inserted into MS+6-BA 0.8mg L -1 +NAA0.4mg·L -1 +30g·L -1 Sucrose+5.5g·L -1 Advent...

Embodiment 3

[0066] 1) Establishment of sterile system and preparation of multiplication materials

[0067]Collect the young stems with joints of Rhizoma sagitta in the same year, brush the dirt attached to the surface of the explants with detergent water, and rinse them under tap water for 30 minutes. Cut into 2.0-3.0cm long stems with 1-2 nodes; soak in 75% alcohol for 30s on a clean bench, and then use 0.1% HgCl 2 Sterilize for 6 minutes, rinse with sterile water 4 times. Inoculated in the addition of 2.0g·L -1 MS basic medium of polyvinylpyrrolidone PVP (Murashige and Skoog, 1962) + 30g L -1 Sucrose+5.5g·L -1 On agar (pH 6.0); after about 10 days of culture and observation, transfer sterile and well-growing stem segments into MS+6-BA0.1mg·L -1 +NAA0.4mg·L -1 +30g·L -1 Sucrose+5.5g·L -1 Axillary buds were induced in agar medium (pH 6.0), and when the axillary buds germinated and grew to 2.0 cm, they were cut and inserted into MS+6-BA 0.8mg L -1 +NAA0.4mg·L -1 +30g·L -1 Sucrose...

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Abstract

The invention belongs to the field of plant regeneration, and provides a culture method for rapid propagation of cotinus coggygria 'royal purple'. The culture method comprises the steps: 1) establishment of a sterile system and preparation of a propagation material, 2) propagation culture: introducing the propagation material into a culture vessel of a plant micro-propagation device, and culturing, wherein the formula of a liquid culture medium is MS+6-BA+NAA; and 3) rooting culture: replacing the liquid culture medium with a liquid rooting culture medium, wherein the formula of the rooting culture medium is 1 / 2MS+IBA(0.5-1.0) mg.L<-1>, and the culture medium volume is 100-150 ml. With intermittent immersion culture, the culture object is not immersed in the liquid culture medium in a whole course, so that adverse effects on plant growth and morphologies due to long term immersion in the liquid are avoided, and the seedling quality is improved. The intermittent immersion culture can provide adequate nutrient and oxygen supplies, reduces accumulation of phenol substances and other unfavorable factors and unfavorable gas produced by cotinus coggygria 'royal purple' seedlings, and makes the cultured object rapidly growing.

Description

technical field [0001] The invention belongs to the field of plant regeneration, and in particular relates to a method for woody plant regeneration through tissue culture technology. Background technique [0002] Cotinus coggygria'Royal Purple' is a woody ornamental plant, belonging to the Anacardiaceae genus Cotinus, a deciduous shrub or small tree. Always red, and changes with seasons: in early spring, all the leaves of the tree are fresh and tender wine red; at the turn of spring and summer, the leaves are red and bright; in midsummer, the lower leaves of the tree gradually turn green, while the top tips The new leaves are always purplish red; after autumn, the overall leaf color gradually turns to deep wine red, and after the autumn frost, the leaf color becomes more red. Every summer, American red cotinus blooms on the top of the branches, and the inflorescences are bright red, like smoke, so it is also called "smoke tree". Compared with common sumac (Cotinus Coggygri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郑彩霞石琨于镇榕
Owner BEIJING FORESTRY UNIVERSITY
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