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Pig akirin2 gene promoter and application thereof

A promoter and gene technology, applied in the field of biochemistry, can solve the problems of endangering transgenic individuals, waste, accumulation, etc.

Inactive Publication Date: 2014-10-15
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

While traditional constitutive promoters promote the expression of exogenous genes, they also cause accumulation and waste of target genes in other tissues and organs, and even endanger transgenic individuals
There is no report about the promoter of akirin2 gene in pigs

Method used

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  • Pig akirin2 gene promoter and application thereof
  • Pig akirin2 gene promoter and application thereof
  • Pig akirin2 gene promoter and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0014] The technical solution of the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0015] The genetic resources involved in the present invention are binary healthy pigs crossed between Large White pigs and Landrace pigs, which are collected in Henan Province, China.

[0016] 1 Materials and methods

[0017] 1.1 Sample collection

[0018] The muscle tissue of healthy pigs was taken, wrapped in tin foil, frozen quickly in liquid nitrogen, and stored in a -80°C low-temperature refrigerator for later use.

[0019] main instrument

[0020] Desktop high-speed centrifuge (Eppendorf, Germany); temperature gradient PCR instrument (Sensoquest, Germany); gel imaging system (AlphaImager HP, USA); trace nucleic acid protein detector NanoDrop-1000 (Thermo, USA); carbon dioxide incubator (MCO -5AC, Sanyo); Inverted Microscope (Leica, Germany); Smart Fluorescence Cell Analyzer (JuLi, Korea); Fluroskan ...

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Abstract

The invention discloses a pig akirin2 gene promoter and application thereof, and the pig akirin2 gene promoter has a nucleotide sequence as shown in SEQ:ID:1. The pig akirin2 gene promoter is used in exogenous gene eukaryotic expression and transgenic pigs. The invention determines that 200bp smallest promoter fragment is most active. The present invention provides expression methods of oligonucleotide primer sequences, cloning vectors, expression vectors, transformed hosts and exogenous proteins. The basis for further research on regulation of the pig akirin2 gene in porcine muscle satellite cells and other related cells is provided, and the pig akirin2 gene also can be used in exogenous protein expression in mammalian cells and transgenic researches.

Description

technical field [0001] The invention belongs to the technical field of biochemistry, and relates to a porcine akirin2 gene promoter and application thereof. Background technique [0002] In the process of growth and development, organisms can express appropriate protein molecules in a timely manner according to environmental changes and their own needs. This often requires a certain program to regulate gene expression. The expression regulation of eukaryotic genes can be divided into several levels and levels, including DNA level, RNA level and protein level. The RNA level includes transcription initiation, post-transcriptional processing and degradation (RNAi is gene expression regulation at this level). It is generally believed that the regulation of transcription initiation is the key point of gene expression regulation, and the most important and important regulation occurs at the initiation stage of transcription. Eukaryotes affect the activity of RNA polymerase thro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/85A01K67/027
Inventor 郭豫杰杨国宇高会贞王月影钟凯鲁维飞韩立强张超
Owner HENAN AGRICULTURAL UNIVERSITY
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