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Preparation method for biocompatible magnetic microspheres collaboratively modified by two macromolecules

A technology of biocompatibility and magnetic microspheres, which is applied in chemical instruments and methods, alkali metal compounds, alkali metal oxides/hydroxides, etc., can solve problems such as blanks in the technical field, and achieve simple preparation methods and high particle size Uniform distribution and improved adsorption efficiency

Active Publication Date: 2014-10-15
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report on the preparation of composite gene carriers by using two kinds of biodegradable polymers and PEI polymers to jointly stabilize magnetic particles, and the technical field of preparing composite magnetic gene carriers by one-step method for drug / gene carrier research is still blank

Method used

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  • Preparation method for biocompatible magnetic microspheres collaboratively modified by two macromolecules
  • Preparation method for biocompatible magnetic microspheres collaboratively modified by two macromolecules
  • Preparation method for biocompatible magnetic microspheres collaboratively modified by two macromolecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Accurately weigh 0.32g γ-PGA into a three-necked flask, add ethylene glycol, and heat and stir at 70°C for 20min in a heating mantle;

[0026] 2. Accurately weigh 0.15g of PEI with a molecular weight of 10,000 in a centrifuge tube, add ethylene glycol, and sonicate until completely dissolved;

[0027] 3. Accurately weigh 0.579g FeCl 3 ·6H 2 O, 1.5g of ammonium acetate is dissolved in ethylene glycol, ultrasonically dispersed evenly, after adding the mixture in step 2, continue ultrasonically dispersed evenly.

[0028] 4. Add the mixed solution in step 3 to the system in step 1, blow in nitrogen, and stir for 1 hour at 160°C;

[0029] 5. After step 4 is completed, quickly add the mixed solution into the reaction kettle, the heating temperature is 220°C, and the reaction time is 16.5 hours to obtain a composite magnetic microsphere solution;

[0030] 6. Disperse the inorganic magnetic microsphere solution obtained in step 5 in alcohol, apply ultrasound for 10 minute...

Embodiment 2

[0032] 1. Accurately weigh 0.214g of chitosan into a three-necked flask, add ethylene glycol, and heat and stir at 60°C for 30min in a heating mantle;

[0033] 2. Accurately weigh 0.15g of PEI with a molecular weight of 1800 in a centrifuge tube, add ethylene glycol agent, and sonicate until completely dissolved;

[0034] 3. Accurately weigh 0.579g Fe(NO 3 ) 3 1.5g of ammonium carbonate was dissolved in ethylene glycol, ultrasonically dispersed evenly, after adding the mixture in step 2, continued ultrasonically dispersed evenly.

[0035] 4. Add the mixed solution in step 3 to the system in step 1, blow in nitrogen, and stir for 1.5 hours at 120°C;

[0036] 5. After step 4 is completed, quickly add the mixed solution into the reaction kettle, heat at 200°C, and react for 24 hours to obtain a composite magnetic microsphere solution;

[0037] 6. Disperse the inorganic magnetic microsphere solution obtained in step 5 in alcohol, apply ultrasound for 15 minutes, use a magnetic ...

Embodiment 3

[0039] 1. Accurately weigh 0.214 γ-PGA into a three-necked flask, add ethylene glycol, and heat and stir at 60°C in a heating mantle for 20 minutes;

[0040]2. Accurately weigh 0.15g of PEI with a molecular weight of 1800 in a centrifuge tube, add ethylene glycol, and sonicate until completely dissolved;

[0041] 3. Accurately weigh 0.579g FeCl 3 ·6H 2 O. Dissolve 1.5g of ammonium acetate in ethylene glycol and disperse evenly by ultrasonic. After adding the mixture in step 2, continue to disperse evenly by ultrasonic.

[0042] 4. Add the mixed solution in step 3 to the system in step 1, blow in nitrogen, and stir for 1 hour at 160°C;

[0043] 5. After step 4 is completed, quickly add the mixed solution into the reaction kettle, the heating temperature is 190°C, and the reaction time is 12 hours to obtain a composite magnetic microsphere solution;

[0044] 6. Disperse the inorganic magnetic microsphere solution obtained in step 5 in alcohol, apply ultrasonication for 5 minu...

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Abstract

The invention relates to a preparation method for biocompatible magnetic microspheres collaboratively modified by two macromolecules and suitable for carrying of medicines or genes. The preparation method is simple, the experiment repeatability is high, and that magnetic microspheres are modified by two macromolecules in situ can be realized in the preparation process. According to the requirements of carrying of the medicines and the genes, the magnetic microspheres have the following properties simultaneously: the content of magnetic materials is high, surface biocompatibility and macromolecule modification are achieved, the magnetic microspheres are formed by assembling nano-crystal grains, and the intergranular gaps are obvious, so that carrying of medicines or gene substances is facilitated, and the magnetic microspheres have good colloid stability in water and PBS; the particle diameter of each magnetic microsphere is 100-250 nanometers, the sizes of original crystal grains and the assembled microspheres can be regulated, the macromolecule coating quantity can be regulated, and the assembly compactness of the crystal grains can be regulated, so that the novel method for preparing magnetic micro-nano materials provided with biocompatibility and capable of effectively carrying the medicines or the genes is provided.

Description

technical field [0001] The invention relates to the technical field of preparation of composite magnetic nanocarriers, in particular to a method for preparing biocompatible magnetic microspheres co-modified by two kinds of macromolecules that can be used to carry microRNA (miRNA). Background technique [0002] In recent years, due to the rapid development of biotechnology, superparamagnetic nanoparticles have shown great promise in the fields of separation of biomolecules such as DNA, protein, polypeptide, and bacteria, magnetic biomarkers, magnetic resonance imaging, targeted drugs, and magnetic superheating for cancer treatment. The application prospect of magnetic nanoparticles in the biological field has become one of the most attractive and dynamic research fields in the world. [0003] In gene therapy, due to the many defects of viral vectors, non-viral vectors such as cationic liposomes and cationic copolymers have received more and more attention. Cationic polymers h...

Claims

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Application Information

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IPC IPC(8): B01J20/26B01J20/28B01J20/30
Inventor 王祎龙李恬韩筱时东陆
Owner TONGJI UNIV
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