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A method for inferring the time interval between human beings after death

A technology of death time and time, which is applied in the field of medical examination, can solve the problem of no gallbladder to reflect the interval time after death, and achieve the effect of easy extraction and storage, overcoming subjectivity, and ensuring accuracy

Active Publication Date: 2017-05-10
JIANGHAN UNIVERSITY
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Problems solved by technology

[0004] In order to solve the problem that the gallbladder does not reflect the postmortem interval in the prior art, the embodiment of the present invention provides a method for inferring the postmortem interval of the human body

Method used

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  • A method for inferring the time interval between human beings after death
  • A method for inferring the time interval between human beings after death
  • A method for inferring the time interval between human beings after death

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Embodiment

[0036] 30 cases of isolated human gallbladder tissues with exact death time in Hubei in 2013 were selected. The age of the deceased was mostly 30±10 years old. Among them, there were 28 cases of males (93.33% of the total) and 2 cases of females (6.67% of the total). ). The cadavers before sampling were stored in an environment of 16-25°C. The temperature of the experimental environment for sampling was 14.5-25°C. The first collection of samples was completed 5 hours after the death of the human body.

[0037] The complete gallbladder was taken out under sterile surgical conditions, placed in normal saline containing 400mg / L gentamicin, stored in a 18°C ​​incubator for 0.5h, and then cut 0.5 1 cm × 0.5 cm × 1 cm sample, and cut it once every 1 hour, immerse the cut sample in clean water and shake to wash, clean the plasma components and blood cells, take out the sample and dry it with filter paper, hold it with tweezers and dry it The sample of the sample was smeared and appl...

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Abstract

The invention discloses a method for estimating the interval time after death of a human body, which belongs to the field of medical examination. The method comprises: extracting human gallbladder mucosal epithelial cells at different death times as samples, and smearing the samples; staining the samples after the smears; performing image analysis on the stained samples, and analyzing parameters Including: heteromorphic index, grayscale parameters and geometric parameters of the nucleus of gallbladder mucosal epithelial cells, grayscale parameters include average optical density, average grayscale, and integrated optical density; perform statistics on the parameters to obtain the linear regression equation of the parameters, according to the linear regression One or more of the equations determine the postmortem interval. The method for estimating the time interval after death of a human body provided by the present invention is to obtain the abnormality index, grayscale parameter and geometrical parameter by analyzing the nuclei of the epithelial cells of the gallbladder mucosa within 5 to 36 hours after the death of the human body. The relationship between the parameters and PMI, so as to infer the interval time after death of the human body.

Description

technical field [0001] The invention relates to the field of medical examination, in particular to a method for estimating the interval time after death of a human body. Background technique [0002] The postmortem interval (PMI) estimation of the human body has always been one of the key points, difficulties and hot spots in the research of forensic pathology, especially the early PMI inference, which is used in judicial practice such as screening criminal suspects in criminal investigation and arbitrating death medical disputes. more realistic. Objective and accurate early PMI inference can provide important objective clues for criminal investigations by public security organs, which is helpful for public security organs to identify whether criminal suspects have time to commit crimes, determine or reduce the scope of investigation, and improve the efficiency and accuracy of investigations. In addition, the inference of the interval after death is sometimes crucial for th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
CPCG01N1/30G01N15/0227G01N21/84G01N2015/0065
Inventor 李长雷杨友华易卉玲舒细记
Owner JIANGHAN UNIVERSITY
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