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Method for cultivating transgenic gramineous plant with increased tiller number and biological material related with method

A technology of gramineous plants and tiller number, applied in the biological field, can solve the problems of smaller leaves, loss of apical dominance, and inability to form yields, etc.

Inactive Publication Date: 2014-10-08
CHINA AGRI UNIV
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Problems solved by technology

The results of the study showed that the transgenic plants with constitutive overexpression of ipt showed increased cytokinin content, delayed leaf senescence, and many abnormal changes in the growth and development of the plants, such as smaller leaves, rounder leaves, Loss of apical dominance, failure to form roots or elongation of formed roots, etc., some will form dwarf plants with many tillers, the total biomass will decrease, and the yield cannot be formed

Method used

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  • Method for cultivating transgenic gramineous plant with increased tiller number and biological material related with method
  • Method for cultivating transgenic gramineous plant with increased tiller number and biological material related with method
  • Method for cultivating transgenic gramineous plant with increased tiller number and biological material related with method

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Experimental program
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Effect test

Embodiment 1

[0047] Embodiment 1, cultivating the transgenic rice that effective tiller number increases and plant height reduces

[0048] 1.1 Construction of pCambia-1301-PMI-nos vector

[0049] PMI gene CDs sequences with XhoI enzyme recognition sites at both ends shown in SEQ ID No.3 are artificially synthesized.

[0050] Replace the hpt gene sequence between the two XhoI enzyme recognition sites on the pCambia-1301-UbiN vector with the 11-1186 positions of SEQ ID No.3 (the first and 3' after the 5' end XhoI enzyme recognition site The first position before the end XhoI enzyme recognition site) of the PMI gene sequence, keep other sequences unchanged, the recombinant expression vector obtained is named as pCambia-1301-PMI, then use EcoRI and KpnI endonuclease to transfer the nos terminator from pCambia Cut off the -1301-UbiN vector and connect it to the EcoRI and KpnI sites of the pCambia-1301-PMI vector, keep other sequences on the pCambia-1301-PMI vector unchanged, and the resulting ...

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Abstract

The invention discloses a method for cultivating a transgenic gramineous plant with increased tiller number and a biological material related with the method. The method comprises the following steps: guiding a DNA (Deoxyribonucleic Acid) molecule PGH3-IPT into a receptor-gramineous plant to obtain the transgenic gramineous plant with increased tiller number, increased spike number and / or reduced plant height in comparison with the receptor-gramineous plant. Compared with the receptor-gramineous plant, the transgenic gramineous plant obtained by guiding the DNA molecule PGH3-IPT into the receptor-gramineous plant has the characteristics that malformation caused by excess change of plant morphology can not be caused when the effective tiller number and plant height of the transgenic gramineous plant are properly reduced; the spike number and grain weight of the transgenic gramineous plant are not remarkably changed, so that the yield of a single gramineous plant is increased; other morphology and growth and development characteristics of the plant are not remarkably changed.

Description

technical field [0001] The invention relates to a method for cultivating transgenic gramineous plants with increased tiller number and related biological materials in the field of biotechnology. Background technique [0002] The improvement of rice yield has always been the goal pursued by breeders, and the components of rice yield include the number of panicles, the number of grains per panicle and the weight of grains, and the number of panicles, especially the effective number of panicles, depends on the number of tillers and reasonable biomass. There are many tiller genes in rice genes, but often due to too many tillers, the total biomass is less, and each tiller is weak and short, and it is difficult to form an effective panicle. With the development of molecular biology, using transgenic technology to introduce exogenous good genes into plants is an important way to improve the efficiency of crop breeding. At the same time, the expression of exogenous genes must be re...

Claims

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Application Information

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IPC IPC(8): C12N15/54C12N9/10C12N15/82C12N5/10A01H5/00
Inventor 付永彩李倩倩朱作峰谭禄宾刘凤霞张坤侯晶晶才宏伟孙传清
Owner CHINA AGRI UNIV
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