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Molecular marker closely linked with rice bacterial blight resistance gene and primers and application thereof

A rice bacterial blight and molecular marker technology is applied in the field of detecting rice bacterial blight resistance, which can solve the problems of long breeding cycle and low selection efficiency, achieve intuitive detection, improve detection efficiency, and improve rice yield level Effect

Inactive Publication Date: 2014-10-08
HEFEI FENGLE SEED
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Conventional breeding methods based on phenotypic selection have disadvantages such as low selection efficiency and long breeding cycle. It is urgent to inject modern molecular technology, supplemented by high-efficiency genotype-directed selection, in order to quickly and efficiently breed excellent new rice varieties.

Method used

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  • Molecular marker closely linked with rice bacterial blight resistance gene and primers and application thereof
  • Molecular marker closely linked with rice bacterial blight resistance gene and primers and application thereof
  • Molecular marker closely linked with rice bacterial blight resistance gene and primers and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Example 1. Acquisition of the special molecular marker BYK1 for the identification of bacterial blight resistance traits

[0024] 1. Sequence search and primer design

[0025] Through the hybridization of the susceptible material IR24 and the disease-resistant material IRBB5, the research population was expanded to analyze the recombination events in the region where the xa5 gene was located, and the "IR24×IRBB5" population of 2345 strains was scanned, and the gene was located in an open reading frame. Sequence analysis showed that the encoded product might be the small subunit of TFIIAγ. The primer was designed using the software Primer Premier5.0 and named BYK1, which consisted of forward primer BYK-F1, forward primer BYK-F2 and reverse primer BYK-R. The corresponding sequence information is respectively SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3.

[0026] SEQ ID NO.1(5,-3,) gccattcaagttcttgag

[0027] SEQ ID NO.2(5,-3,)agctcgccattcaagttcttgtc

[0028] SEQ ID NO.3(5,...

Embodiment 2

[0032]Example 2, Correlation verification between the amplification products of BYK primer pairs and resistance traits

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Abstract

The invention discloses a molecular marker closely linked with rice bacterial blight resistance gene and primers and an application thereof. The molecular marker is characterized in that the nucleotide sequence of the molecular marker is shown in SEQ ID NO: 4. The nucleotide sequence of a primer 1 of a primer pair is shown in SEQ ID NO: 1 and the nucleotide sequence of a primer 2 is shown in SEQ ID NO: 2. When the primers are used for rice bacterial blight detection, after PCR (polymerase chain reaction) amplification, if the PCR amplification product is a 98bp base fragment, the rice to be detected is a bacterial blight resistant variety; if the PCR amplification product is a 103bp base fragment, the rice to be detected is a bacterial blight susceptible variety. The method disclosed by the invention can be used for implementing early generational selection on the bacterial blight resistant rice to shorten the breeding cycle so that a bacterial blight resistant rice material can be screened out quickly; therefore, the method can be widely applied to assistant breeding of the rice.

Description

technical field [0001] The invention belongs to the technical field of plant disease resistance breeding, and in particular relates to a method for detecting rice bacterial blight resistance, a function-specific molecular marker and applications thereof. Background technique [0002] Conventional breeding methods based on phenotypic selection have disadvantages such as low selection efficiency and long breeding cycle. It is urgent to inject modern molecular technology, supplemented by high-efficiency genotype-directed selection, in order to quickly and efficiently breed excellent new rice varieties. With the rapid development of molecular biology and genomics, the application of molecular marker technology is more extensive. PCR-based molecular markers such as microsatellites or SSR (simple sequence repeat) are widely used because of their high polymorphism rate, relative stability, simple and fast detection method, and easy operation. Since molecular marker-assisted select...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 朱先飞徐继萍蒋继武陈庆全王兆贤周桂林徐剑汪大海陈满元唐照锐
Owner HEFEI FENGLE SEED
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