Preparation method of fructus momordicae extract applicable to industrial production

Abstract

The invention discloses a preparation method of fructus momordicae extract applicable to industrial production. The preparation method comprises the following steps: (1) washing and crushing fresh fruits of fructus momordicae; (2) adding a hydrophilic solvent, heating, conducting reflux extraction and filtering to obtain extracted liquor; (3) heating the extracted liquor, conducting vacuum concentration, cooling, standing and filtering to obtain concentrated liquor; (4) conducting tubular centrifugation to the concentrated liquor and then ultrafiltering to obtain filtrate; (5) conducting column adsorption by connecting more than three macroporous resin chromatographic columns to the filtrate, standing and washing, eluting respectively through the hydrophilic solvent and respectively collecting eluent; (6) respectively conducting nanofiltration to the eluent through nanofiltering films, and then concentrating until the brix degree is about 25-30, to obtain concentrated liquor; and (7) spray-drying the concentrated liquor, to obtain the fructus momordicae extract. By utilizing the method, a plurality of specifications of mogroside V extract products can be produced at a time, resin can be efficiently utilized, energy consumption can be reduced, the quality of the product can be guaranteed, the cost can be lowered, and the fructus momordicae extract is applicable to industrial production.

Description

technical field [0001] The invention relates to a preparation method of Luo Han Guo extract, in particular to a preparation method of Luo Han Guo extract suitable for industrial production. Background technique [0002] Luo Han Guo, Latin name Corvenor Momordica fruit, also known as Lahan Guo and False Bitter Gourd, is a plant of the genus Cucurbitaceae. It is distributed in Guangxi, Guangdong, Hunan and other provinces in southern China. It is mainly produced in the mountainous areas of Lingui County and Yongfu County in Guilin City. Luo Han Guo contains glycosides, polysaccharides, flavonoids, proteins and amino acids, as well as vitamin C and mineral elements. As a medicinal and edible plant, Luo Han Guo can be used as medicine and edible. Its dried fruit is often used as traditional Chinese medicine, which has good effects of clearing away heat, relieving heat, moistening lung and relieving cough. In addition, Luo Han Guo can be used as a food additive in various dessert...

AI Technical Summary

Problems solved by technology

[0003] Since Luo Han Guo is a unique plant species in my country and has obvious geographical advantages, the research on the preparation of Luo Han Guo extracts is mainly concentrated in China, and there are many related patent literature reports, but few of them can be really applied to production, mainly reflected in the following aspects : (1) The process is cumbersome and the yield is low; (2) A large amount of organic solvent is used, which requires high equipment and large investment; (3) The process consumes a lot of energy and costs; (4) The introduction of other substances in the process affects the product quality

For example, CN101029071B discloses the method for preparing high-purity mogroside V from Luo Han Guo. The method is to carry out alcohol extraction, concentration, extraction with ethyl acetate and n-butanol to the dried fruit of Luo Han Guo, and then use silica gel column, C18 reverse phase silica gel column chromatography 1. After drying, high-purity sweet glycoside V products are obtained. The main defects of this method are cumbersome process, large solvent loss and low yield, which is not conducive to large-scale industrial production; CN100572552C discloses mogroside V ≥ 40% Luo Han Guo extract and its preparation The method is to extract the fresh fruit of Luo Han Guo with water, concentrate, enzymolyze, absorb it with a macroporous resin, decolorize it with an ion exchange resin, and finally concentrate and dry it to obtain the product. Because this method does not filter before loading the column, the subsequent processing cost of the resin is Larger, not conducive to continuous production; CN101407535B discloses a preparation method of high-purity mogroside V, the method is to use a certain purity of Luo Han Guo extract as raw material, dissolved in methanol, acetone extraction, normal phase silica gel adsorption, separation , crystallization and recrystallization to obtain the final product. This method has a simple process, but a large amount of organic solvents are used in the process, and the requirements for the production site are relatively high. In addition, the obtained products also have certain organic solvent residues, which affect the quality; CN101863946B discloses a A method for extracting high-content mogroside V from dried grosvenor fruit. The method is to crush the dried grosvenor fruit, carry out alcohol extraction, concentration, centrifugation, macroporous resin adsorption separation, ion exchange resin decolorization, and finally concentration and drying to obtain a finished product. Because this method is not filtered, there are more macromolecular impurities in the upper column liquid, which affects the adsorption performance of the resin, the utilization rate is low, the follow-up treatment is relatively difficult, and the product cost is high.