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A kind of extraction method of magnolia total sesquiterpene lactone extract

The technology of a sesquiterpene lactone and an extraction method is applied in the field of extraction of the total sesquiterpene lactone extract of Manchurian lotus, can solve the problems of a large amount of time, low effective components, etc., and achieves low cost, easy industrial production, and high content. Effect

Inactive Publication Date: 2016-01-13
HAINAN VOCATIONAL COLLEGE OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the existing technologies for the extraction of active ingredients of medicinal plants use soaking methods, which often require a lot of time and use a lot of solvents, and the effective ingredients dissolved are relatively low

Method used

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  • A kind of extraction method of magnolia total sesquiterpene lactone extract
  • A kind of extraction method of magnolia total sesquiterpene lactone extract
  • A kind of extraction method of magnolia total sesquiterpene lactone extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1, extracting the total sesquiterpene lactones of Magnolia magnolia leaves as raw material

[0027] Wash the collected fresh Mangosteen leaf samples and dry them in a dry and ventilated place for 14 days to obtain dried leaves, crush them; weigh 10.0g of crushed dried leaves, add 100mL85% ethanol to a 250mL small beaker, extract them by ultrasonic wave at room temperature for 30min, and pour them out Supernatant; extract the sample twice again with the same method, filter the sample after the third ultrasonic extraction, and combine the three ultrasonic extractions. Wash the filter residue three times with 30mL85% ethanol solution, combine the washing liquid and the extract, and concentrate the filtrate to 50mL by vacuum distillation; The extract of the ester layer is distilled under reduced pressure to obtain the extract, which is the crude extract. The above operation was repeated 10 times, and the extracts were combined to obtain 4.953 g of crude extract....

Embodiment 2

[0032] Example 2. Extracting total sesquiterpene lactones rich in parthenolide from the bark of Magnolia hainanensis as raw material

[0033] Wash the collected fresh mangosteen bark samples and dry them in a dry and ventilated place for 10 days to obtain dry products, crush them; weigh 10.0 g of the crushed dry products, add 80 mL of 90% methanol to a 250 mL small beaker, and extract them by ultrasonic waves at room temperature for 40 minutes, then pour them out Supernatant; the same method was used to extract the sample three times, and after the fourth ultrasonic extraction, the sample was filtered, and the four ultrasonic extractions were combined. Wash the filter residue three times with 30 mL of 90% methanol solution, combine the washing liquid and the extract, and concentrate the filtrate to 60 mL by vacuum distillation; extract the concentrate three times with 180 mL of methyl acetate, and combine three times of methyl acetate The extract of the ester layer was distill...

Embodiment 3

[0036] Example 3: Extracting the total sesquiterpene lactones of Magnolia magnolia hainanensis with stems as raw materials and purifying the active ingredients

[0037] Carry out ultrasonic extraction with the method in Example 1 with 1000g dried stems of Magnolia hainanensis to obtain 59.7849g of extract (crude extract), dissolve the extract with ethanol and mix the sample with 30-60 mesh silica gel, pass 200-300 Mesh, 80×100cm dry silica gel column, eluted with 10:1 petroleum ether and ethyl acetate solution until the color becomes lighter, then eluted with 3:1 petroleum ether and ethyl acetate solution to combine eluent, HPLC tracking Detection, collect fractions containing parthenolide and distill to dryness under reduced pressure, dissolve with as little methanol as possible, pass through 80×100cm C 18 Reverse-phase silica gel column, eluted with 4:6 methanol water solution until the color becomes light, and then eluted with 7:3 methanol water solution elution system, HPL...

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Abstract

The invention belongs to the technical field of plant extraction, and relates to a method for extracting a manglietia fordiana total sesquiterpene lactone extract. The method comprises the following steps: crushing a dry sample of a manglietia fordiana plant material, and adding hydrous lower alcohol to perform ultrasonic extraction; filtering, performing pressure reduction and solvent recovery on filtrate, and extracting to obtain a crude extract by using an ester solvent; and then performing column chromatography separation and purification on the crude extract, and collecting required components to obtain the manglietia fordiana total sesquiterpene lactone extract. The method disclosed by the invention is simple in process, can be used for avoiding parthenolide decomposition and damages caused by long-term immersion by adopting an ultrasonic extraction technology, can be used for saving the extraction time, and has the advantages of high yield, low cost and easy industrial production; and the obtained extract is high in parthenolide content and clear in effective components, has anti-tumor effects, and can be used for producing various medicinal preparations with the anti-tumor effects.

Description

technical field [0001] The invention belongs to the technical field of plant extraction, and relates to a method for extracting total sesquiterpene lactones, in particular to a method for extracting total sesquiterpene lactones from Magnolia magnolia. Background technique [0002] Parthenolide (English name: parthenolide, molecular formula C 15 h 2 OO 3 , relative molecular weight 248.32, melting point 115-116) ℃ is a kind of sesquiterpene lactone compound (sesquiterpenelactone) purified from tansy at the earliest, and it is also one of the main components with the highest value in tansy extract. Studies have reported that parthenolide has anti-tumor effects. Pharmacological studies have shown that parthenolide is an effective inhibitor of the transcription factor NF-κB. It inhibits the normal activation of NF-κB by blocking the degradation of IκB-α and IκB-β, and specifically inhibits the β-β-β in the IκB kinase complex. IκB subunit. Parthenolide can also inhibit the b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D493/04
CPCC07D493/04
Inventor 闫浩张敬畅劳厚波杜金风曹维良徐雪峰张玉
Owner HAINAN VOCATIONAL COLLEGE OF SCI & TECH
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